Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3201 and GFF3202 are separated by 104 nucleotides GFF3202 and GFF3203 are separated by 23 nucleotides GFF3203 and GFF3204 are separated by 112 nucleotides
GFF3201 - LysR-family transcriptional regulator YhaJ, at 205,147 to 206,043
GFF3201
GFF3202 - Pirin-like protein YhaK, at 206,148 to 206,849
GFF3202
GFF3203 - Uncharacterized protein YhaL, at 206,873 to 207,037
GFF3203
GFF3204 - Inner membrane protein, at 207,150 to 208,460
GFF3204
Position (kb) 206
207 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 205.162 kb on + strand at 205.163 kb on - strand at 205.163 kb on - strand at 205.171 kb on + strand at 205.230 kb on + strand at 205.354 kb on - strand, within GFF3201 at 205.370 kb on - strand, within GFF3201 at 205.523 kb on + strand, within GFF3201 at 205.523 kb on + strand, within GFF3201 at 205.523 kb on + strand, within GFF3201 at 205.524 kb on - strand, within GFF3201 at 205.539 kb on + strand, within GFF3201 at 205.542 kb on - strand, within GFF3201 at 205.840 kb on - strand, within GFF3201 at 205.840 kb on - strand, within GFF3201 at 205.863 kb on + strand, within GFF3201 at 205.864 kb on - strand, within GFF3201 at 205.889 kb on - strand, within GFF3201 at 205.889 kb on - strand, within GFF3201 at 206.129 kb on + strand at 206.144 kb on - strand at 206.213 kb on + strand at 206.214 kb on - strand at 206.233 kb on - strand, within GFF3202 at 206.395 kb on + strand, within GFF3202 at 206.396 kb on - strand, within GFF3202 at 206.449 kb on - strand, within GFF3202 at 206.459 kb on + strand, within GFF3202 at 206.463 kb on - strand, within GFF3202 at 206.606 kb on - strand, within GFF3202 at 206.629 kb on + strand, within GFF3202 at 206.630 kb on - strand, within GFF3202 at 206.630 kb on - strand, within GFF3202 at 206.636 kb on + strand, within GFF3202 at 206.636 kb on + strand, within GFF3202 at 206.637 kb on - strand, within GFF3202 at 206.639 kb on + strand, within GFF3202 at 206.639 kb on + strand, within GFF3202 at 206.711 kb on + strand, within GFF3202 at 206.795 kb on + strand at 206.839 kb on + strand at 206.840 kb on - strand at 206.848 kb on - strand at 206.910 kb on + strand, within GFF3203 at 207.152 kb on - strand at 207.236 kb on + strand at 207.401 kb on + strand, within GFF3204 at 207.402 kb on - strand, within GFF3204 at 207.423 kb on - strand, within GFF3204 at 207.442 kb on - strand, within GFF3204 at 207.529 kb on + strand, within GFF3204 at 207.530 kb on - strand, within GFF3204 at 207.530 kb on - strand, within GFF3204 at 207.534 kb on - strand, within GFF3204 at 207.556 kb on - strand, within GFF3204 at 207.627 kb on - strand, within GFF3204
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 205,162 + +0.8 205,163 - -0.6 205,163 - +0.9 205,171 + +0.0 205,230 + +0.0 205,354 - GFF3201 0.23 -3.1 205,370 - GFF3201 0.25 -0.7 205,523 + GFF3201 0.42 -1.3 205,523 + GFF3201 0.42 +0.3 205,523 + GFF3201 0.42 +0.5 205,524 - GFF3201 0.42 -0.5 205,539 + GFF3201 0.44 +0.0 205,542 - GFF3201 0.44 +0.8 205,840 - GFF3201 0.77 +0.6 205,840 - GFF3201 0.77 -0.9 205,863 + GFF3201 0.80 -1.0 205,864 - GFF3201 0.80 +0.4 205,889 - GFF3201 0.83 -0.9 205,889 - GFF3201 0.83 -0.1 206,129 + +0.0 206,144 - +0.3 206,213 + +1.3 206,214 - -0.0 206,233 - GFF3202 0.12 -0.5 206,395 + GFF3202 0.35 -1.4 206,396 - GFF3202 0.35 +0.2 206,449 - GFF3202 0.43 +1.9 206,459 + GFF3202 0.44 -0.0 206,463 - GFF3202 0.45 -0.8 206,606 - GFF3202 0.65 -0.2 206,629 + GFF3202 0.69 -0.1 206,630 - GFF3202 0.69 +0.6 206,630 - GFF3202 0.69 +0.3 206,636 + GFF3202 0.70 +0.1 206,636 + GFF3202 0.70 -1.4 206,637 - GFF3202 0.70 +0.4 206,639 + GFF3202 0.70 -0.2 206,639 + GFF3202 0.70 -0.8 206,711 + GFF3202 0.80 +0.9 206,795 + +0.4 206,839 + -0.7 206,840 - +1.3 206,848 - -0.9 206,910 + GFF3203 0.22 -1.0 207,152 - -0.5 207,236 + +0.1 207,401 + GFF3204 0.19 -0.0 207,402 - GFF3204 0.19 -0.0 207,423 - GFF3204 0.21 +0.2 207,442 - GFF3204 0.22 +0.2 207,529 + GFF3204 0.29 +0.2 207,530 - GFF3204 0.29 -0.4 207,530 - GFF3204 0.29 -0.8 207,534 - GFF3204 0.29 +1.3 207,556 - GFF3204 0.31 +0.8 207,627 - GFF3204 0.36 +0.3
Or see this region's nucleotide sequence