Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3079 and GFF3080 are separated by 103 nucleotides GFF3080 and GFF3081 overlap by 23 nucleotides
GFF3079 - Putative response regulator, at 89,162 to 89,908
GFF3079
GFF3080 - FIG074102: hypothetical protein, at 90,012 to 90,569
GFF3080
GFF3081 - Unknown, probable amino acid transporter, at 90,547 to 92,046
GFF3081
Position (kb) 90
91 Strain fitness (log2 ratio) -1
0
1
2
3 at 89.111 kb on + strand at 89.124 kb on + strand at 89.180 kb on - strand at 89.278 kb on - strand, within GFF3079 at 89.480 kb on - strand, within GFF3079 at 89.515 kb on + strand, within GFF3079 at 89.524 kb on + strand, within GFF3079 at 89.573 kb on - strand, within GFF3079 at 89.573 kb on - strand, within GFF3079 at 89.605 kb on - strand, within GFF3079 at 89.643 kb on + strand, within GFF3079 at 89.902 kb on + strand at 89.903 kb on - strand at 89.958 kb on + strand at 89.960 kb on + strand at 90.065 kb on + strand at 90.078 kb on - strand, within GFF3080 at 90.137 kb on + strand, within GFF3080 at 90.145 kb on - strand, within GFF3080 at 90.162 kb on + strand, within GFF3080 at 90.243 kb on + strand, within GFF3080 at 90.266 kb on - strand, within GFF3080 at 90.674 kb on - strand at 90.728 kb on - strand, within GFF3081 at 90.729 kb on + strand, within GFF3081 at 90.729 kb on + strand, within GFF3081 at 90.730 kb on - strand, within GFF3081 at 90.730 kb on - strand, within GFF3081 at 90.733 kb on + strand, within GFF3081 at 90.733 kb on + strand, within GFF3081 at 90.734 kb on - strand, within GFF3081 at 90.768 kb on + strand, within GFF3081 at 90.771 kb on + strand, within GFF3081 at 90.784 kb on - strand, within GFF3081 at 90.861 kb on + strand, within GFF3081 at 90.865 kb on + strand, within GFF3081 at 90.866 kb on - strand, within GFF3081 at 90.866 kb on - strand, within GFF3081 at 90.897 kb on - strand, within GFF3081 at 90.901 kb on + strand, within GFF3081 at 90.902 kb on - strand, within GFF3081 at 90.977 kb on - strand, within GFF3081 at 91.082 kb on + strand, within GFF3081 at 91.083 kb on - strand, within GFF3081 at 91.084 kb on + strand, within GFF3081 at 91.086 kb on + strand, within GFF3081 at 91.117 kb on - strand, within GFF3081 at 91.203 kb on + strand, within GFF3081 at 91.216 kb on - strand, within GFF3081 at 91.296 kb on + strand, within GFF3081 at 91.296 kb on + strand, within GFF3081 at 91.296 kb on + strand, within GFF3081 at 91.297 kb on - strand, within GFF3081 at 91.297 kb on - strand, within GFF3081 at 91.297 kb on - strand, within GFF3081 at 91.424 kb on + strand, within GFF3081
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 89,111 + +0.2 89,124 + -0.7 89,180 - +0.4 89,278 - GFF3079 0.16 -0.0 89,480 - GFF3079 0.43 +0.3 89,515 + GFF3079 0.47 -0.2 89,524 + GFF3079 0.48 +0.9 89,573 - GFF3079 0.55 -0.2 89,573 - GFF3079 0.55 +0.2 89,605 - GFF3079 0.59 +1.0 89,643 + GFF3079 0.64 -0.1 89,902 + -0.0 89,903 - +0.2 89,958 + -0.6 89,960 + +0.0 90,065 + -0.3 90,078 - GFF3080 0.12 +2.9 90,137 + GFF3080 0.22 -0.1 90,145 - GFF3080 0.24 +0.4 90,162 + GFF3080 0.27 +0.4 90,243 + GFF3080 0.41 +0.1 90,266 - GFF3080 0.46 +0.5 90,674 - +0.3 90,728 - GFF3081 0.12 -0.1 90,729 + GFF3081 0.12 +0.2 90,729 + GFF3081 0.12 -0.1 90,730 - GFF3081 0.12 -1.0 90,730 - GFF3081 0.12 -0.6 90,733 + GFF3081 0.12 +0.0 90,733 + GFF3081 0.12 +0.6 90,734 - GFF3081 0.12 -0.5 90,768 + GFF3081 0.15 -0.9 90,771 + GFF3081 0.15 +0.2 90,784 - GFF3081 0.16 -0.1 90,861 + GFF3081 0.21 +0.4 90,865 + GFF3081 0.21 -0.1 90,866 - GFF3081 0.21 +2.0 90,866 - GFF3081 0.21 +0.4 90,897 - GFF3081 0.23 +2.2 90,901 + GFF3081 0.24 +1.5 90,902 - GFF3081 0.24 +0.3 90,977 - GFF3081 0.29 -0.3 91,082 + GFF3081 0.36 +0.3 91,083 - GFF3081 0.36 -1.1 91,084 + GFF3081 0.36 -0.6 91,086 + GFF3081 0.36 -0.5 91,117 - GFF3081 0.38 +1.1 91,203 + GFF3081 0.44 +0.7 91,216 - GFF3081 0.45 +0.7 91,296 + GFF3081 0.50 +0.6 91,296 + GFF3081 0.50 +0.2 91,296 + GFF3081 0.50 +0.1 91,297 - GFF3081 0.50 -0.6 91,297 - GFF3081 0.50 +0.2 91,297 - GFF3081 0.50 +0.2 91,424 + GFF3081 0.58 -0.7
Or see this region's nucleotide sequence