Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3022 and GFF3023 are separated by 373 nucleotides GFF3023 and GFF3024 are separated by 25 nucleotides GFF3024 and GFF3025 are separated by 0 nucleotides
GFF3022 - D-erythrose-4-phosphate dehydrogenase (EC 1.2.1.72), at 37,011 to 38,057
GFF3022
GFF3023 - Predicted regulator of STY3230 transporter operon, at 38,431 to 38,856
GFF3023
GFF3024 - Substrate-specific component STY3230 of queuosine-regulated ECF transporter, at 38,882 to 39,460
GFF3024
GFF3025 - Transmembrane component STY3231 of energizing module of queuosine-regulated ECF transporter, at 39,461 to 40,168
GFF3025
Position (kb) 38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 37.465 kb on - strand, within GFF3022 at 37.566 kb on - strand, within GFF3022 at 37.578 kb on + strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.752 kb on + strand, within GFF3022 at 37.752 kb on + strand, within GFF3022 at 37.753 kb on - strand, within GFF3022 at 37.753 kb on - strand, within GFF3022 at 37.761 kb on - strand, within GFF3022 at 38.050 kb on - strand at 38.057 kb on + strand at 38.057 kb on + strand at 38.057 kb on + strand at 38.058 kb on - strand at 38.103 kb on - strand at 38.103 kb on - strand at 38.216 kb on + strand at 38.250 kb on + strand at 38.251 kb on - strand at 38.300 kb on + strand at 38.318 kb on - strand at 38.364 kb on - strand at 38.389 kb on + strand at 38.389 kb on + strand at 38.389 kb on + strand at 38.390 kb on - strand at 38.390 kb on - strand at 38.439 kb on - strand at 38.439 kb on - strand at 38.439 kb on - strand at 38.457 kb on + strand at 38.514 kb on - strand, within GFF3023 at 38.541 kb on - strand, within GFF3023 at 38.730 kb on + strand, within GFF3023 at 38.731 kb on - strand, within GFF3023 at 38.745 kb on + strand, within GFF3023 at 38.842 kb on - strand at 38.882 kb on - strand at 38.932 kb on + strand at 38.944 kb on + strand, within GFF3024 at 38.945 kb on - strand, within GFF3024 at 38.945 kb on - strand, within GFF3024 at 38.945 kb on - strand, within GFF3024 at 38.946 kb on - strand, within GFF3024 at 39.009 kb on + strand, within GFF3024 at 39.013 kb on + strand, within GFF3024 at 39.061 kb on - strand, within GFF3024 at 39.208 kb on + strand, within GFF3024 at 39.209 kb on - strand, within GFF3024 at 39.348 kb on + strand, within GFF3024 at 39.349 kb on - strand, within GFF3024 at 39.368 kb on - strand, within GFF3024 at 39.421 kb on + strand at 39.459 kb on - strand at 39.486 kb on + strand at 39.546 kb on - strand, within GFF3025 at 39.605 kb on + strand, within GFF3025 at 39.606 kb on - strand, within GFF3025 at 39.606 kb on - strand, within GFF3025 at 39.651 kb on + strand, within GFF3025 at 39.652 kb on - strand, within GFF3025 at 39.732 kb on + strand, within GFF3025 at 39.733 kb on - strand, within GFF3025 at 39.765 kb on + strand, within GFF3025 at 39.765 kb on + strand, within GFF3025 at 39.766 kb on - strand, within GFF3025 at 39.766 kb on - strand, within GFF3025 at 39.776 kb on + strand, within GFF3025 at 39.777 kb on - strand, within GFF3025 at 39.777 kb on - strand, within GFF3025 at 39.840 kb on - strand, within GFF3025
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 37,465 - GFF3022 0.43 -1.0 37,566 - GFF3022 0.53 -2.0 37,578 + GFF3022 0.54 -0.4 37,579 - GFF3022 0.54 -1.1 37,579 - GFF3022 0.54 -2.1 37,579 - GFF3022 0.54 -1.0 37,579 - GFF3022 0.54 -2.9 37,752 + GFF3022 0.71 +0.0 37,752 + GFF3022 0.71 -1.0 37,753 - GFF3022 0.71 -1.0 37,753 - GFF3022 0.71 -0.7 37,761 - GFF3022 0.72 -0.8 38,050 - -1.6 38,057 + -0.7 38,057 + +0.7 38,057 + -2.3 38,058 - -1.3 38,103 - +0.4 38,103 - +0.5 38,216 + -0.3 38,250 + +0.2 38,251 - -0.4 38,300 + -0.1 38,318 - -0.5 38,364 - -1.0 38,389 + -0.4 38,389 + +0.1 38,389 + -0.2 38,390 - -0.1 38,390 - +0.3 38,439 - +1.3 38,439 - -0.5 38,439 - -0.2 38,457 + +1.1 38,514 - GFF3023 0.19 +0.2 38,541 - GFF3023 0.26 -0.2 38,730 + GFF3023 0.70 -0.4 38,731 - GFF3023 0.70 -0.7 38,745 + GFF3023 0.74 -0.2 38,842 - -0.3 38,882 - +3.1 38,932 + +1.1 38,944 + GFF3024 0.11 -1.3 38,945 - GFF3024 0.11 -0.1 38,945 - GFF3024 0.11 +0.1 38,945 - GFF3024 0.11 +1.1 38,946 - GFF3024 0.11 +0.3 39,009 + GFF3024 0.22 +1.2 39,013 + GFF3024 0.23 +0.1 39,061 - GFF3024 0.31 +2.8 39,208 + GFF3024 0.56 +0.2 39,209 - GFF3024 0.56 -1.2 39,348 + GFF3024 0.80 +1.5 39,349 - GFF3024 0.81 -0.1 39,368 - GFF3024 0.84 -0.9 39,421 + +0.8 39,459 - +1.0 39,486 + +1.6 39,546 - GFF3025 0.12 -0.4 39,605 + GFF3025 0.20 -0.3 39,606 - GFF3025 0.20 -1.3 39,606 - GFF3025 0.20 +0.0 39,651 + GFF3025 0.27 +0.1 39,652 - GFF3025 0.27 +0.2 39,732 + GFF3025 0.38 -0.3 39,733 - GFF3025 0.38 +0.6 39,765 + GFF3025 0.43 -0.1 39,765 + GFF3025 0.43 +0.8 39,766 - GFF3025 0.43 -0.6 39,766 - GFF3025 0.43 -0.9 39,776 + GFF3025 0.44 -0.8 39,777 - GFF3025 0.45 -0.2 39,777 - GFF3025 0.45 -0.6 39,840 - GFF3025 0.54 -1.2
Or see this region's nucleotide sequence