Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3005 and GFF3006 are separated by 40 nucleotides GFF3006 and GFF3007 are separated by 273 nucleotides GFF3007 and GFF3008 are separated by 161 nucleotides
GFF3005 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 20,722 to 21,816
GFF3005
GFF3006 - hypothetical protein, at 21,857 to 21,997
GFF3006
GFF3007 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 22,271 to 23,437
GFF3007
GFF3008 - 2-octaprenyl-6-methoxyphenol hydroxylase (EC 1.14.13.-), at 23,599 to 24,777
GFF3008
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-1
0
1
2
3 at 21.306 kb on + strand, within GFF3005 at 21.306 kb on + strand, within GFF3005 at 21.306 kb on + strand, within GFF3005 at 21.307 kb on - strand, within GFF3005 at 21.307 kb on - strand, within GFF3005 at 21.307 kb on - strand, within GFF3005 at 21.336 kb on + strand, within GFF3005 at 21.477 kb on + strand, within GFF3005 at 21.509 kb on + strand, within GFF3005 at 21.512 kb on + strand, within GFF3005 at 21.587 kb on - strand, within GFF3005 at 21.614 kb on + strand, within GFF3005 at 21.619 kb on + strand, within GFF3005 at 21.729 kb on - strand at 21.844 kb on - strand at 22.483 kb on - strand, within GFF3007 at 22.650 kb on + strand, within GFF3007 at 22.697 kb on + strand, within GFF3007 at 22.697 kb on + strand, within GFF3007 at 22.724 kb on - strand, within GFF3007 at 22.766 kb on - strand, within GFF3007 at 22.766 kb on - strand, within GFF3007 at 22.844 kb on - strand, within GFF3007 at 22.844 kb on - strand, within GFF3007 at 23.070 kb on - strand, within GFF3007 at 23.095 kb on + strand, within GFF3007 at 23.130 kb on + strand, within GFF3007 at 23.131 kb on - strand, within GFF3007 at 23.197 kb on + strand, within GFF3007 at 23.200 kb on + strand, within GFF3007 at 23.277 kb on - strand, within GFF3007 at 23.456 kb on + strand at 23.457 kb on - strand at 23.579 kb on + strand at 23.657 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 21,306 + GFF3005 0.53 +0.1 21,306 + GFF3005 0.53 -0.2 21,306 + GFF3005 0.53 +3.2 21,307 - GFF3005 0.53 -0.4 21,307 - GFF3005 0.53 +1.0 21,307 - GFF3005 0.53 +0.4 21,336 + GFF3005 0.56 +0.9 21,477 + GFF3005 0.69 +0.1 21,509 + GFF3005 0.72 -0.3 21,512 + GFF3005 0.72 +0.0 21,587 - GFF3005 0.79 +0.6 21,614 + GFF3005 0.81 +0.5 21,619 + GFF3005 0.82 +0.2 21,729 - -0.2 21,844 - -0.1 22,483 - GFF3007 0.18 +2.2 22,650 + GFF3007 0.32 +2.0 22,697 + GFF3007 0.37 +1.7 22,697 + GFF3007 0.37 +2.4 22,724 - GFF3007 0.39 +3.5 22,766 - GFF3007 0.42 +2.1 22,766 - GFF3007 0.42 +2.6 22,844 - GFF3007 0.49 +1.3 22,844 - GFF3007 0.49 +2.2 23,070 - GFF3007 0.68 +2.2 23,095 + GFF3007 0.71 +2.2 23,130 + GFF3007 0.74 +2.9 23,131 - GFF3007 0.74 +2.6 23,197 + GFF3007 0.79 +2.5 23,200 + GFF3007 0.80 +2.1 23,277 - GFF3007 0.86 +1.7 23,456 + +2.0 23,457 - +2.3 23,579 + +2.0 23,657 - +1.3
Or see this region's nucleotide sequence