Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2989 and GFF2990 are separated by 5 nucleotides GFF2990 and GFF2991 are separated by 23 nucleotides
GFF2989 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 7,083 to 8,816
GFF2989
GFF2990 - Thiol:disulfide interchange protein DsbC, at 8,822 to 9,535
GFF2990
GFF2991 - Tyrosine recombinase XerD, at 9,559 to 10,455
GFF2991
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 7.919 kb on + strand, within GFF2989 at 8.024 kb on + strand, within GFF2989 at 8.081 kb on + strand, within GFF2989 at 8.258 kb on + strand, within GFF2989 at 8.467 kb on - strand, within GFF2989 at 8.504 kb on - strand, within GFF2989 at 8.730 kb on + strand at 8.742 kb on + strand at 8.742 kb on + strand at 8.742 kb on + strand at 8.824 kb on - strand at 8.824 kb on - strand at 8.824 kb on - strand at 8.901 kb on + strand, within GFF2990 at 8.902 kb on - strand, within GFF2990 at 8.916 kb on - strand, within GFF2990 at 8.961 kb on + strand, within GFF2990 at 9.007 kb on + strand, within GFF2990 at 9.058 kb on + strand, within GFF2990 at 9.068 kb on - strand, within GFF2990 at 9.114 kb on + strand, within GFF2990 at 9.139 kb on + strand, within GFF2990 at 9.161 kb on + strand, within GFF2990 at 9.161 kb on + strand, within GFF2990 at 9.162 kb on - strand, within GFF2990 at 9.162 kb on - strand, within GFF2990 at 9.231 kb on + strand, within GFF2990 at 9.337 kb on + strand, within GFF2990 at 9.337 kb on + strand, within GFF2990 at 9.338 kb on - strand, within GFF2990 at 9.361 kb on - strand, within GFF2990 at 9.718 kb on + strand, within GFF2991 at 9.719 kb on - strand, within GFF2991 at 9.719 kb on - strand, within GFF2991 at 9.767 kb on + strand, within GFF2991 at 9.856 kb on + strand, within GFF2991 at 10.028 kb on + strand, within GFF2991 at 10.044 kb on + strand, within GFF2991 at 10.045 kb on - strand, within GFF2991 at 10.491 kb on + strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 7,919 + GFF2989 0.48 -0.6 8,024 + GFF2989 0.54 -0.1 8,081 + GFF2989 0.58 -0.2 8,258 + GFF2989 0.68 +0.1 8,467 - GFF2989 0.80 -0.8 8,504 - GFF2989 0.82 -1.0 8,730 + -0.6 8,742 + -0.8 8,742 + -1.0 8,742 + -0.3 8,824 - -1.0 8,824 - -1.0 8,824 - -0.6 8,901 + GFF2990 0.11 -1.4 8,902 - GFF2990 0.11 -2.2 8,916 - GFF2990 0.13 -2.1 8,961 + GFF2990 0.19 -1.2 9,007 + GFF2990 0.26 -2.7 9,058 + GFF2990 0.33 -3.2 9,068 - GFF2990 0.34 -3.4 9,114 + GFF2990 0.41 -3.0 9,139 + GFF2990 0.44 -0.2 9,161 + GFF2990 0.47 -3.7 9,161 + GFF2990 0.47 -2.6 9,162 - GFF2990 0.48 -0.1 9,162 - GFF2990 0.48 -1.4 9,231 + GFF2990 0.57 -2.7 9,337 + GFF2990 0.72 -3.3 9,337 + GFF2990 0.72 -2.7 9,338 - GFF2990 0.72 -1.8 9,361 - GFF2990 0.75 -1.6 9,718 + GFF2991 0.18 -0.9 9,719 - GFF2991 0.18 +1.3 9,719 - GFF2991 0.18 -2.4 9,767 + GFF2991 0.23 -1.8 9,856 + GFF2991 0.33 -0.7 10,028 + GFF2991 0.52 -1.0 10,044 + GFF2991 0.54 -2.1 10,045 - GFF2991 0.54 -1.5 10,491 + -0.7 10,492 - -0.6 10,492 - -0.2 10,492 - +3.2 10,492 - -0.4
Or see this region's nucleotide sequence