Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2617

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2616 and GFF2617 are separated by 186 nucleotidesGFF2617 and GFF2618 are separated by 31 nucleotides GFF2616 - Methyl-accepting chemotaxis protein III (ribose and galactose chemoreceptor protein), at 141,497 to 143,104 GFF2616 GFF2617 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 143,291 to 144,409 GFF2617 GFF2618 - FrmR: Negative transcriptional regulator of formaldehyde detoxification operon, at 144,441 to 144,716 GFF2618 Position (kb) 143 144 145Strain fitness (log2 ratio) -2 -1 0 1at 142.487 kb on - strand, within GFF2616at 142.523 kb on - strand, within GFF2616at 142.547 kb on + strand, within GFF2616at 142.598 kb on - strand, within GFF2616at 142.600 kb on - strand, within GFF2616at 142.699 kb on + strand, within GFF2616at 142.771 kb on + strand, within GFF2616at 142.771 kb on + strand, within GFF2616at 142.772 kb on - strand, within GFF2616at 142.898 kb on - strand, within GFF2616at 142.901 kb on + strand, within GFF2616at 143.148 kb on - strandat 143.575 kb on + strand, within GFF2617at 143.654 kb on - strand, within GFF2617at 143.665 kb on + strand, within GFF2617at 143.923 kb on + strand, within GFF2617at 143.979 kb on + strand, within GFF2617at 144.124 kb on + strand, within GFF2617at 144.147 kb on + strand, within GFF2617at 144.246 kb on - strand, within GFF2617at 144.246 kb on - strand, within GFF2617at 144.275 kb on - strand, within GFF2617at 144.302 kb on - strandat 144.411 kb on + strandat 144.443 kb on - strandat 144.446 kb on + strandat 144.686 kb on + strand, within GFF2618at 144.733 kb on - strandat 144.946 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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142,487 - GFF2616 0.62 +0.2
142,523 - GFF2616 0.64 -0.2
142,547 + GFF2616 0.65 +0.2
142,598 - GFF2616 0.68 +0.6
142,600 - GFF2616 0.69 +1.1
142,699 + GFF2616 0.75 -0.7
142,771 + GFF2616 0.79 +0.5
142,771 + GFF2616 0.79 -0.7
142,772 - GFF2616 0.79 -0.1
142,898 - GFF2616 0.87 +0.1
142,901 + GFF2616 0.87 +0.9
143,148 - -0.4
143,575 + GFF2617 0.25 -0.4
143,654 - GFF2617 0.32 -2.3
143,665 + GFF2617 0.33 -0.7
143,923 + GFF2617 0.56 +0.2
143,979 + GFF2617 0.61 -0.4
144,124 + GFF2617 0.74 +0.5
144,147 + GFF2617 0.76 -0.7
144,246 - GFF2617 0.85 +0.3
144,246 - GFF2617 0.85 -0.3
144,275 - GFF2617 0.88 -0.0
144,302 - -0.1
144,411 + -0.2
144,443 - -1.4
144,446 + +0.6
144,686 + GFF2618 0.89 -0.3
144,733 - +1.2
144,946 + +0.1

Or see this region's nucleotide sequence