Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2606 and GFF2607 are separated by 11 nucleotides GFF2607 and GFF2608 are separated by 9 nucleotides GFF2608 and GFF2609 overlap by 1 nucleotides GFF2609 and GFF2610 are separated by 46 nucleotides
GFF2606 - PTS system, IIA component, at 131,088 to 131,525
GFF2606
GFF2607 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 131,537 to 132,169
GFF2607
GFF2608 - Putative arylsulfatase regulatory protein, at 132,179 to 132,976
GFF2608
GFF2609 - 'Aminoglycoside 6'-N-acetyltransferase' transl_table=11, at 132,976 to 133,413
GFF2609
GFF2610 - Lactate 2-monooxygenase (EC 1.13.12.4), at 133,460 to 134,662
GFF2610
Position (kb)
132
133 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 131.213 kb on + strand, within GFF2606 at 131.214 kb on - strand, within GFF2606 at 131.229 kb on - strand, within GFF2606 at 131.241 kb on + strand, within GFF2606 at 131.242 kb on - strand, within GFF2606 at 131.242 kb on - strand, within GFF2606 at 131.242 kb on - strand, within GFF2606 at 131.321 kb on + strand, within GFF2606 at 131.326 kb on + strand, within GFF2606 at 131.659 kb on + strand, within GFF2607 at 131.666 kb on - strand, within GFF2607 at 131.671 kb on - strand, within GFF2607 at 131.702 kb on + strand, within GFF2607 at 131.840 kb on - strand, within GFF2607 at 132.117 kb on + strand at 132.165 kb on + strand at 132.178 kb on + strand at 132.178 kb on + strand at 132.314 kb on + strand, within GFF2608 at 132.316 kb on + strand, within GFF2608 at 132.317 kb on - strand, within GFF2608 at 132.358 kb on + strand, within GFF2608 at 132.359 kb on - strand, within GFF2608 at 132.609 kb on - strand, within GFF2608 at 132.609 kb on - strand, within GFF2608 at 132.703 kb on + strand, within GFF2608 at 132.704 kb on - strand, within GFF2608 at 132.748 kb on + strand, within GFF2608 at 132.749 kb on - strand, within GFF2608 at 132.781 kb on + strand, within GFF2608 at 132.782 kb on - strand, within GFF2608 at 132.804 kb on + strand, within GFF2608 at 132.837 kb on + strand, within GFF2608 at 132.838 kb on - strand, within GFF2608 at 132.871 kb on + strand, within GFF2608 at 132.871 kb on + strand, within GFF2608 at 132.884 kb on - strand, within GFF2608 at 133.441 kb on - strand at 133.458 kb on + strand at 133.604 kb on - strand, within GFF2610 at 133.793 kb on - strand, within GFF2610 at 133.886 kb on - strand, within GFF2610 at 133.892 kb on + strand, within GFF2610 at 133.893 kb on - strand, within GFF2610 at 133.893 kb on - strand, within GFF2610
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 131,213 + GFF2606 0.29 +0.4 131,214 - GFF2606 0.29 +0.1 131,229 - GFF2606 0.32 -0.4 131,241 + GFF2606 0.35 -0.8 131,242 - GFF2606 0.35 -0.3 131,242 - GFF2606 0.35 -0.7 131,242 - GFF2606 0.35 -0.9 131,321 + GFF2606 0.53 -1.7 131,326 + GFF2606 0.54 +0.8 131,659 + GFF2607 0.19 +0.1 131,666 - GFF2607 0.20 +0.3 131,671 - GFF2607 0.21 -1.2 131,702 + GFF2607 0.26 +0.1 131,840 - GFF2607 0.48 -0.3 132,117 + -0.8 132,165 + +0.1 132,178 + -0.2 132,178 + +1.3 132,314 + GFF2608 0.17 +0.6 132,316 + GFF2608 0.17 +1.0 132,317 - GFF2608 0.17 +0.3 132,358 + GFF2608 0.22 +0.1 132,359 - GFF2608 0.23 -0.0 132,609 - GFF2608 0.54 +3.0 132,609 - GFF2608 0.54 -0.5 132,703 + GFF2608 0.66 +0.1 132,704 - GFF2608 0.66 +0.6 132,748 + GFF2608 0.71 +0.4 132,749 - GFF2608 0.71 -0.0 132,781 + GFF2608 0.75 -0.4 132,782 - GFF2608 0.76 +1.1 132,804 + GFF2608 0.78 +0.7 132,837 + GFF2608 0.82 +0.1 132,838 - GFF2608 0.83 -1.2 132,871 + GFF2608 0.87 +0.9 132,871 + GFF2608 0.87 +0.4 132,884 - GFF2608 0.88 +0.0 133,441 - +0.1 133,458 + +2.2 133,604 - GFF2610 0.12 +0.7 133,793 - GFF2610 0.28 +0.0 133,886 - GFF2610 0.35 +1.3 133,892 + GFF2610 0.36 +0.6 133,893 - GFF2610 0.36 -2.1 133,893 - GFF2610 0.36 -0.2
Or see this region's nucleotide sequence