Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2588 and GFF2589 are separated by 114 nucleotides GFF2589 and GFF2590 are separated by 135 nucleotides
GFF2588 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 112,452 to 113,897
GFF2588
GFF2589 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 114,012 to 115,436
GFF2589
GFF2590 - D-alanyl-D-alanine dipeptidase (EC 3.4.13.22), at 115,572 to 116,342
GFF2590
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 113.018 kb on - strand, within GFF2588 at 113.022 kb on - strand, within GFF2588 at 113.022 kb on - strand, within GFF2588 at 113.037 kb on - strand, within GFF2588 at 113.056 kb on + strand, within GFF2588 at 113.183 kb on + strand, within GFF2588 at 113.201 kb on + strand, within GFF2588 at 113.307 kb on - strand, within GFF2588 at 113.486 kb on + strand, within GFF2588 at 113.486 kb on + strand, within GFF2588 at 113.870 kb on - strand at 113.877 kb on - strand at 114.314 kb on + strand, within GFF2589 at 114.376 kb on + strand, within GFF2589 at 114.376 kb on + strand, within GFF2589 at 114.581 kb on - strand, within GFF2589 at 114.753 kb on - strand, within GFF2589 at 114.780 kb on - strand, within GFF2589 at 114.822 kb on + strand, within GFF2589 at 114.998 kb on + strand, within GFF2589 at 115.167 kb on - strand, within GFF2589 at 115.167 kb on - strand, within GFF2589 at 115.176 kb on + strand, within GFF2589 at 115.244 kb on - strand, within GFF2589 at 115.269 kb on - strand, within GFF2589 at 115.317 kb on - strand at 115.629 kb on - strand at 115.629 kb on - strand at 115.692 kb on + strand, within GFF2590 at 115.696 kb on - strand, within GFF2590 at 115.703 kb on + strand, within GFF2590 at 115.823 kb on + strand, within GFF2590 at 115.852 kb on + strand, within GFF2590 at 115.854 kb on + strand, within GFF2590 at 116.050 kb on + strand, within GFF2590 at 116.060 kb on - strand, within GFF2590 at 116.072 kb on + strand, within GFF2590 at 116.154 kb on - strand, within GFF2590 at 116.156 kb on - strand, within GFF2590 at 116.232 kb on - strand, within GFF2590 at 116.232 kb on - strand, within GFF2590 at 116.232 kb on - strand, within GFF2590 at 116.235 kb on - strand, within GFF2590 at 116.337 kb on + strand at 116.380 kb on - strand at 116.392 kb on - strand at 116.424 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 113,018 - GFF2588 0.39 -0.5 113,022 - GFF2588 0.39 -1.2 113,022 - GFF2588 0.39 -0.5 113,037 - GFF2588 0.40 -2.4 113,056 + GFF2588 0.42 -0.4 113,183 + GFF2588 0.51 +0.8 113,201 + GFF2588 0.52 +1.1 113,307 - GFF2588 0.59 +0.3 113,486 + GFF2588 0.72 -1.8 113,486 + GFF2588 0.72 +0.7 113,870 - -0.4 113,877 - +0.6 114,314 + GFF2589 0.21 -0.0 114,376 + GFF2589 0.26 +2.5 114,376 + GFF2589 0.26 -0.5 114,581 - GFF2589 0.40 -0.1 114,753 - GFF2589 0.52 -1.0 114,780 - GFF2589 0.54 -1.4 114,822 + GFF2589 0.57 -0.1 114,998 + GFF2589 0.69 +0.9 115,167 - GFF2589 0.81 +0.9 115,167 - GFF2589 0.81 -0.5 115,176 + GFF2589 0.82 -0.3 115,244 - GFF2589 0.86 -0.6 115,269 - GFF2589 0.88 -0.6 115,317 - +0.2 115,629 - +0.6 115,629 - -0.0 115,692 + GFF2590 0.16 -0.1 115,696 - GFF2590 0.16 -0.6 115,703 + GFF2590 0.17 -0.1 115,823 + GFF2590 0.33 +0.4 115,852 + GFF2590 0.36 +0.3 115,854 + GFF2590 0.37 +0.3 116,050 + GFF2590 0.62 +0.2 116,060 - GFF2590 0.63 -0.9 116,072 + GFF2590 0.65 -0.3 116,154 - GFF2590 0.75 +0.0 116,156 - GFF2590 0.76 -0.6 116,232 - GFF2590 0.86 +0.3 116,232 - GFF2590 0.86 -0.3 116,232 - GFF2590 0.86 +0.5 116,235 - GFF2590 0.86 -0.6 116,337 + +0.0 116,380 - +0.2 116,392 - -0.4 116,424 + -0.4
Or see this region's nucleotide sequence