Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2538 and GFF2539 are separated by 151 nucleotides GFF2539 and GFF2540 overlap by 11 nucleotides GFF2540 and GFF2541 are separated by 121 nucleotides GFF2541 and GFF2542 are separated by 407 nucleotides
GFF2538 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 52,456 to 52,704
GFF2538
GFF2539 - putative phage-related secreted protein, at 52,856 to 53,071
GFF2539
GFF2540 - FIG01046001: hypothetical protein, at 53,061 to 53,393
GFF2540
GFF2541 - putative cytoplasmic protein, at 53,515 to 54,444
GFF2541
GFF2542 - putative transposase, at 54,852 to 55,214
GFF2542
Position (kb) 53
54
55 Strain fitness (log2 ratio) -1
0
1
2
3 at 52.830 kb on - strand at 52.909 kb on - strand, within GFF2539 at 53.194 kb on + strand, within GFF2540 at 53.262 kb on + strand, within GFF2540 at 53.263 kb on - strand, within GFF2540 at 53.348 kb on + strand, within GFF2540 at 53.349 kb on - strand, within GFF2540 at 53.524 kb on + strand at 53.764 kb on - strand, within GFF2541 at 54.093 kb on - strand, within GFF2541 at 54.114 kb on - strand, within GFF2541 at 54.281 kb on + strand, within GFF2541 at 54.285 kb on + strand, within GFF2541 at 54.730 kb on + strand at 54.743 kb on + strand at 55.250 kb on + strand at 55.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 52,830 - -0.5 52,909 - GFF2539 0.25 +0.0 53,194 + GFF2540 0.40 -0.4 53,262 + GFF2540 0.60 +0.8 53,263 - GFF2540 0.61 +3.3 53,348 + GFF2540 0.86 +0.3 53,349 - GFF2540 0.86 +2.0 53,524 + +0.6 53,764 - GFF2541 0.27 -0.6 54,093 - GFF2541 0.62 +1.4 54,114 - GFF2541 0.64 -0.5 54,281 + GFF2541 0.82 -1.1 54,285 + GFF2541 0.83 +3.0 54,730 + +2.0 54,743 + +3.4 55,250 + +0.4 55,366 - +0.1
Or see this region's nucleotide sequence