Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2469 and GFF2470 are separated by 37 nucleotides GFF2470 and GFF2471 are separated by 111 nucleotides GFF2471 and GFF2472 overlap by 1 nucleotides GFF2472 and GFF2473 are separated by 11 nucleotides GFF2473 and GFF2474 are separated by 11 nucleotides
GFF2469 - Transcriptional regulator, LysR family, at 89,211 to 90,137
GFF2469
GFF2470 - 4Fe-4S ferredoxin, iron-sulfur binding, at 90,175 to 90,435
GFF2470
GFF2471 - Holo-[acyl-carrier protein] synthase (EC 2.7.8.7), at 90,547 to 90,927
GFF2471
GFF2472 - 'Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)' transl_table=11, at 90,927 to 91,658
GFF2472
GFF2473 - DNA recombination and repair protein RecO, at 91,670 to 92,398
GFF2473
GFF2474 - GTP-binding protein Era, at 92,410 to 93,315
GFF2474
Position (kb) 90
91
92 Strain fitness (log2 ratio) -1
0
1
2 at 90.082 kb on + strand at 90.083 kb on - strand at 90.136 kb on - strand at 90.214 kb on - strand, within GFF2470 at 90.510 kb on - strand at 90.528 kb on - strand at 90.548 kb on + strand at 91.761 kb on - strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 92.137 kb on + strand, within GFF2473 at 92.221 kb on - strand, within GFF2473 at 92.423 kb on - strand at 92.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 90,082 + -0.2 90,083 - -0.4 90,136 - -1.2 90,214 - GFF2470 0.15 -0.7 90,510 - -0.5 90,528 - +0.9 90,548 + +0.5 91,761 - GFF2473 0.12 -1.1 91,802 + GFF2473 0.18 +1.9 91,802 + GFF2473 0.18 +1.2 91,802 + GFF2473 0.18 +2.3 91,802 + GFF2473 0.18 +1.0 92,137 + GFF2473 0.64 +1.5 92,221 - GFF2473 0.76 -0.9 92,423 - +0.1 92,423 - +1.2
Or see this region's nucleotide sequence