Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2455 and GFF2456 overlap by 4 nucleotides GFF2456 and GFF2457 are separated by 79 nucleotides GFF2457 and GFF2458 are separated by 307 nucleotides GFF2458 and GFF2459 are separated by 258 nucleotides
GFF2455 - Putative sensory histidine kinase YfhA, at 72,559 to 73,896
GFF2455
GFF2456 - putative alpha helix protein, at 73,893 to 74,579
GFF2456
GFF2457 - Putative sensor-like histidine kinase YfhK, at 74,659 to 76,044
GFF2457
GFF2458 - hypothetical protein, at 76,352 to 76,480
GFF2458
GFF2459 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 76,739 to 80,626
GFF2459
Position (kb)
74
75
76
77 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 73.702 kb on - strand, within GFF2455 at 73.760 kb on - strand, within GFF2455 at 73.760 kb on - strand, within GFF2455 at 73.762 kb on + strand, within GFF2455 at 73.985 kb on - strand, within GFF2456 at 73.985 kb on - strand, within GFF2456 at 74.054 kb on + strand, within GFF2456 at 74.135 kb on + strand, within GFF2456 at 74.136 kb on - strand, within GFF2456 at 74.221 kb on - strand, within GFF2456 at 74.221 kb on - strand, within GFF2456 at 74.327 kb on + strand, within GFF2456 at 74.327 kb on + strand, within GFF2456 at 74.488 kb on + strand, within GFF2456 at 74.572 kb on - strand at 74.660 kb on + strand at 74.661 kb on - strand at 74.757 kb on + strand at 74.883 kb on + strand, within GFF2457 at 75.013 kb on + strand, within GFF2457 at 75.013 kb on + strand, within GFF2457 at 75.014 kb on - strand, within GFF2457 at 75.046 kb on - strand, within GFF2457 at 75.093 kb on + strand, within GFF2457 at 75.158 kb on + strand, within GFF2457 at 75.288 kb on + strand, within GFF2457 at 75.329 kb on - strand, within GFF2457 at 75.381 kb on + strand, within GFF2457 at 75.382 kb on - strand, within GFF2457 at 75.544 kb on + strand, within GFF2457 at 75.599 kb on - strand, within GFF2457 at 75.627 kb on + strand, within GFF2457 at 75.711 kb on + strand, within GFF2457 at 75.736 kb on + strand, within GFF2457 at 75.759 kb on - strand, within GFF2457 at 75.765 kb on + strand, within GFF2457 at 75.765 kb on + strand, within GFF2457 at 75.765 kb on + strand, within GFF2457 at 75.817 kb on - strand, within GFF2457 at 75.830 kb on + strand, within GFF2457 at 75.831 kb on - strand, within GFF2457 at 75.875 kb on + strand, within GFF2457 at 76.147 kb on - strand at 76.229 kb on + strand at 76.230 kb on - strand at 76.230 kb on - strand at 76.289 kb on - strand at 76.313 kb on - strand at 76.581 kb on - strand at 76.654 kb on + strand at 76.655 kb on - strand at 76.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 73,702 - GFF2455 0.85 +1.9 73,760 - GFF2455 0.90 +1.7 73,760 - GFF2455 0.90 +1.5 73,762 + GFF2455 0.90 +2.2 73,985 - GFF2456 0.13 -0.5 73,985 - GFF2456 0.13 -1.1 74,054 + GFF2456 0.23 +2.7 74,135 + GFF2456 0.35 +2.2 74,136 - GFF2456 0.35 +1.3 74,221 - GFF2456 0.48 +2.9 74,221 - GFF2456 0.48 +2.5 74,327 + GFF2456 0.63 +1.8 74,327 + GFF2456 0.63 +1.0 74,488 + GFF2456 0.87 +2.8 74,572 - +1.6 74,660 + +1.5 74,661 - -1.3 74,757 + +1.9 74,883 + GFF2457 0.16 +3.9 75,013 + GFF2457 0.26 +1.7 75,013 + GFF2457 0.26 +2.0 75,014 - GFF2457 0.26 +0.8 75,046 - GFF2457 0.28 +1.4 75,093 + GFF2457 0.31 +1.8 75,158 + GFF2457 0.36 +2.1 75,288 + GFF2457 0.45 +1.4 75,329 - GFF2457 0.48 +1.9 75,381 + GFF2457 0.52 +0.9 75,382 - GFF2457 0.52 +0.7 75,544 + GFF2457 0.64 +0.8 75,599 - GFF2457 0.68 +1.0 75,627 + GFF2457 0.70 +1.9 75,711 + GFF2457 0.76 +1.2 75,736 + GFF2457 0.78 +1.2 75,759 - GFF2457 0.79 +1.6 75,765 + GFF2457 0.80 +1.9 75,765 + GFF2457 0.80 +2.3 75,765 + GFF2457 0.80 +1.6 75,817 - GFF2457 0.84 +1.6 75,830 + GFF2457 0.84 +1.7 75,831 - GFF2457 0.85 +1.2 75,875 + GFF2457 0.88 +1.0 76,147 - -0.5 76,229 + -1.6 76,230 - +0.9 76,230 - -0.4 76,289 - +0.1 76,313 - -0.5 76,581 - -0.2 76,654 + -2.3 76,655 - +0.0 76,930 - +1.3
Or see this region's nucleotide sequence