Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2443 and GFF2444 overlap by 10 nucleotides GFF2444 and GFF2445 are separated by 95 nucleotides GFF2445 and GFF2446 overlap by 6 nucleotides
GFF2443 - putative membrane protein, at 57,818 to 58,804
GFF2443
GFF2444 - FIG00923183: possible membrane protein YebY, at 58,795 to 59,463
GFF2444
GFF2445 - Stationary phase inducible protein CsiE, at 59,559 to 60,836
GFF2445
GFF2446 - Probable 3-phenylpropionic acid transporter, at 60,831 to 61,970
GFF2446
Position (kb) 59
60
61 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 58.739 kb on + strand at 58.833 kb on + strand at 58.918 kb on - strand, within GFF2444 at 58.919 kb on + strand, within GFF2444 at 58.920 kb on - strand, within GFF2444 at 58.982 kb on + strand, within GFF2444 at 58.998 kb on - strand, within GFF2444 at 59.000 kb on + strand, within GFF2444 at 59.027 kb on + strand, within GFF2444 at 59.027 kb on + strand, within GFF2444 at 59.027 kb on + strand, within GFF2444 at 59.028 kb on - strand, within GFF2444 at 59.028 kb on - strand, within GFF2444 at 59.166 kb on + strand, within GFF2444 at 59.186 kb on - strand, within GFF2444 at 59.221 kb on + strand, within GFF2444 at 59.302 kb on + strand, within GFF2444 at 59.482 kb on + strand at 59.676 kb on - strand at 59.694 kb on - strand, within GFF2445 at 59.822 kb on - strand, within GFF2445 at 59.848 kb on + strand, within GFF2445 at 59.936 kb on + strand, within GFF2445 at 60.031 kb on - strand, within GFF2445 at 60.041 kb on - strand, within GFF2445 at 60.189 kb on - strand, within GFF2445 at 60.335 kb on + strand, within GFF2445 at 60.348 kb on + strand, within GFF2445 at 60.348 kb on + strand, within GFF2445 at 60.349 kb on - strand, within GFF2445 at 60.349 kb on - strand, within GFF2445 at 60.383 kb on - strand, within GFF2445 at 60.383 kb on - strand, within GFF2445 at 60.405 kb on - strand, within GFF2445 at 60.620 kb on + strand, within GFF2445 at 60.621 kb on - strand, within GFF2445 at 61.029 kb on - strand, within GFF2446 at 61.134 kb on + strand, within GFF2446 at 61.245 kb on + strand, within GFF2446 at 61.245 kb on + strand, within GFF2446 at 61.249 kb on + strand, within GFF2446 at 61.505 kb on - strand, within GFF2446 at 61.511 kb on - strand, within GFF2446
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 58,739 + -0.6 58,833 + -1.3 58,918 - GFF2444 0.18 +0.6 58,919 + GFF2444 0.19 +0.4 58,920 - GFF2444 0.19 +0.1 58,982 + GFF2444 0.28 -0.2 58,998 - GFF2444 0.30 -1.6 59,000 + GFF2444 0.31 +0.0 59,027 + GFF2444 0.35 +1.9 59,027 + GFF2444 0.35 -0.5 59,027 + GFF2444 0.35 +0.7 59,028 - GFF2444 0.35 +0.4 59,028 - GFF2444 0.35 -2.0 59,166 + GFF2444 0.55 -2.0 59,186 - GFF2444 0.58 -0.1 59,221 + GFF2444 0.64 -0.3 59,302 + GFF2444 0.76 +0.4 59,482 + +0.4 59,676 - -0.5 59,694 - GFF2445 0.11 +2.9 59,822 - GFF2445 0.21 +0.1 59,848 + GFF2445 0.23 -0.6 59,936 + GFF2445 0.29 -0.5 60,031 - GFF2445 0.37 +1.5 60,041 - GFF2445 0.38 +0.9 60,189 - GFF2445 0.49 -0.2 60,335 + GFF2445 0.61 +0.2 60,348 + GFF2445 0.62 -0.8 60,348 + GFF2445 0.62 -0.6 60,349 - GFF2445 0.62 -0.4 60,349 - GFF2445 0.62 +0.7 60,383 - GFF2445 0.64 +0.0 60,383 - GFF2445 0.64 -0.6 60,405 - GFF2445 0.66 -0.5 60,620 + GFF2445 0.83 +0.2 60,621 - GFF2445 0.83 +1.3 61,029 - GFF2446 0.17 +0.2 61,134 + GFF2446 0.27 -0.5 61,245 + GFF2446 0.36 -0.6 61,245 + GFF2446 0.36 +0.1 61,249 + GFF2446 0.37 -0.4 61,505 - GFF2446 0.59 -0.0 61,511 - GFF2446 0.60 -1.9
Or see this region's nucleotide sequence