Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2277 and GFF2278 are separated by 11 nucleotides GFF2278 and GFF2279 are separated by 50 nucleotides GFF2279 and GFF2280 overlap by 4 nucleotides GFF2280 and GFF2281 are separated by 150 nucleotides
GFF2277 - Uncharacterized protein conserved in bacteria, at 66,153 to 66,842
GFF2277
GFF2278 - Putative inner membrane protein, at 66,854 to 67,249
GFF2278
GFF2279 - Copper sensory histidine kinase CpxA, at 67,300 to 68,673
GFF2279
GFF2280 - Copper-sensing two-component system response regulator CpxR, at 68,670 to 69,368
GFF2280
GFF2281 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 69,519 to 70,019
GFF2281
Position (kb) 67
68
69 Strain fitness (log2 ratio) -1
0
1
2 at 66.322 kb on + strand, within GFF2277 at 66.323 kb on - strand, within GFF2277 at 66.449 kb on + strand, within GFF2277 at 66.462 kb on + strand, within GFF2277 at 66.522 kb on - strand, within GFF2277 at 66.692 kb on + strand, within GFF2277 at 66.692 kb on + strand, within GFF2277 at 66.693 kb on - strand, within GFF2277 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.939 kb on - strand, within GFF2278 at 66.939 kb on - strand, within GFF2278 at 66.940 kb on + strand, within GFF2278 at 67.001 kb on - strand, within GFF2278 at 67.080 kb on - strand, within GFF2278 at 67.534 kb on - strand, within GFF2279 at 67.536 kb on + strand, within GFF2279 at 67.576 kb on - strand, within GFF2279 at 67.605 kb on + strand, within GFF2279 at 67.644 kb on - strand, within GFF2279 at 67.736 kb on - strand, within GFF2279 at 67.924 kb on + strand, within GFF2279 at 68.134 kb on + strand, within GFF2279 at 68.135 kb on - strand, within GFF2279 at 68.243 kb on + strand, within GFF2279 at 68.515 kb on + strand, within GFF2279 at 68.516 kb on - strand, within GFF2279 at 68.516 kb on - strand, within GFF2279 at 68.612 kb on - strand at 68.612 kb on - strand at 68.692 kb on + strand at 68.769 kb on - strand, within GFF2280 at 68.873 kb on - strand, within GFF2280 at 69.063 kb on - strand, within GFF2280 at 69.144 kb on - strand, within GFF2280 at 69.186 kb on - strand, within GFF2280 at 69.373 kb on + strand at 69.395 kb on + strand at 69.414 kb on - strand at 69.426 kb on + strand at 69.427 kb on - strand at 69.437 kb on - strand at 69.593 kb on + strand, within GFF2281 at 69.593 kb on + strand, within GFF2281 at 69.635 kb on + strand, within GFF2281 at 69.651 kb on - strand, within GFF2281 at 69.651 kb on - strand, within GFF2281
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 66,322 + GFF2277 0.24 +0.1 66,323 - GFF2277 0.25 -0.4 66,449 + GFF2277 0.43 -0.2 66,462 + GFF2277 0.45 +0.6 66,522 - GFF2277 0.53 +0.4 66,692 + GFF2277 0.78 +0.2 66,692 + GFF2277 0.78 -0.0 66,693 - GFF2277 0.78 -0.2 66,938 + GFF2278 0.21 +2.4 66,938 + GFF2278 0.21 +0.1 66,938 + GFF2278 0.21 -0.8 66,938 + GFF2278 0.21 +0.5 66,939 - GFF2278 0.21 +1.0 66,939 - GFF2278 0.21 -1.1 66,940 + GFF2278 0.22 -0.1 67,001 - GFF2278 0.37 +0.2 67,080 - GFF2278 0.57 +1.3 67,534 - GFF2279 0.17 -1.5 67,536 + GFF2279 0.17 +0.6 67,576 - GFF2279 0.20 -0.3 67,605 + GFF2279 0.22 +0.0 67,644 - GFF2279 0.25 +0.0 67,736 - GFF2279 0.32 +0.4 67,924 + GFF2279 0.45 +0.3 68,134 + GFF2279 0.61 +0.9 68,135 - GFF2279 0.61 -0.5 68,243 + GFF2279 0.69 +0.1 68,515 + GFF2279 0.88 +1.7 68,516 - GFF2279 0.89 +0.1 68,516 - GFF2279 0.89 +1.1 68,612 - +0.0 68,612 - -0.1 68,692 + +1.9 68,769 - GFF2280 0.14 +1.6 68,873 - GFF2280 0.29 +1.9 69,063 - GFF2280 0.56 +1.1 69,144 - GFF2280 0.68 +2.1 69,186 - GFF2280 0.74 +1.6 69,373 + +2.5 69,395 + +1.6 69,414 - +0.3 69,426 + +0.9 69,427 - +0.2 69,437 - -0.4 69,593 + GFF2281 0.15 -0.7 69,593 + GFF2281 0.15 -0.9 69,635 + GFF2281 0.23 -0.5 69,651 - GFF2281 0.26 +0.5 69,651 - GFF2281 0.26 -1.0
Or see this region's nucleotide sequence