Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2237 and GFF2238 are separated by 164 nucleotides GFF2238 and GFF2239 are separated by 33 nucleotides
GFF2237 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 32,688 to 33,584
GFF2237
GFF2238 - Sugar kinase YihV, at 33,749 to 34,645
GFF2238
GFF2239 - DeoR-type transcriptional regulator YihW, at 34,679 to 35,482
GFF2239
Position (kb) 33
34
35 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 32.788 kb on + strand, within GFF2237 at 32.976 kb on + strand, within GFF2237 at 33.051 kb on - strand, within GFF2237 at 33.058 kb on + strand, within GFF2237 at 33.058 kb on + strand, within GFF2237 at 33.059 kb on - strand, within GFF2237 at 33.059 kb on - strand, within GFF2237 at 33.153 kb on + strand, within GFF2237 at 33.305 kb on + strand, within GFF2237 at 33.305 kb on + strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.573 kb on + strand at 33.576 kb on - strand at 33.772 kb on + strand at 33.794 kb on + strand at 33.794 kb on + strand at 33.797 kb on + strand at 33.798 kb on - strand at 33.798 kb on - strand at 33.827 kb on - strand at 33.830 kb on + strand at 33.830 kb on + strand at 33.830 kb on + strand at 33.831 kb on - strand at 33.831 kb on - strand at 33.984 kb on + strand, within GFF2238 at 34.092 kb on + strand, within GFF2238 at 34.189 kb on + strand, within GFF2238 at 34.189 kb on + strand, within GFF2238 at 34.234 kb on + strand, within GFF2238 at 34.252 kb on + strand, within GFF2238 at 34.444 kb on + strand, within GFF2238 at 34.466 kb on - strand, within GFF2238 at 34.643 kb on + strand at 34.643 kb on + strand at 34.646 kb on + strand at 34.647 kb on - strand at 34.647 kb on - strand at 34.655 kb on + strand at 34.655 kb on + strand at 34.656 kb on - strand at 34.726 kb on - strand at 34.748 kb on + strand at 34.750 kb on + strand at 34.756 kb on + strand at 34.817 kb on - strand, within GFF2239 at 34.841 kb on - strand, within GFF2239 at 34.957 kb on + strand, within GFF2239 at 34.977 kb on + strand, within GFF2239 at 35.032 kb on + strand, within GFF2239 at 35.033 kb on - strand, within GFF2239 at 35.048 kb on - strand, within GFF2239 at 35.082 kb on - strand, within GFF2239 at 35.082 kb on - strand, within GFF2239 at 35.083 kb on + strand, within GFF2239 at 35.084 kb on - strand, within GFF2239 at 35.084 kb on - strand, within GFF2239 at 35.137 kb on + strand, within GFF2239 at 35.209 kb on - strand, within GFF2239 at 35.297 kb on - strand, within GFF2239 at 35.530 kb on + strand at 35.611 kb on - strand at 35.643 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 32,788 + GFF2237 0.11 +0.3 32,976 + GFF2237 0.32 -0.2 33,051 - GFF2237 0.40 -0.1 33,058 + GFF2237 0.41 -0.9 33,058 + GFF2237 0.41 -0.3 33,059 - GFF2237 0.41 +1.6 33,059 - GFF2237 0.41 +0.5 33,153 + GFF2237 0.52 +2.1 33,305 + GFF2237 0.69 +0.0 33,305 + GFF2237 0.69 +0.2 33,306 - GFF2237 0.69 +2.8 33,306 - GFF2237 0.69 +1.1 33,306 - GFF2237 0.69 -0.4 33,306 - GFF2237 0.69 +0.5 33,306 - GFF2237 0.69 +0.2 33,573 + -1.3 33,576 - -0.4 33,772 + -1.3 33,794 + +0.9 33,794 + +0.0 33,797 + -0.1 33,798 - -0.7 33,798 - +0.2 33,827 - -0.1 33,830 + -0.1 33,830 + -0.6 33,830 + -2.4 33,831 - +0.5 33,831 - -2.6 33,984 + GFF2238 0.26 +0.1 34,092 + GFF2238 0.38 -1.3 34,189 + GFF2238 0.49 +1.1 34,189 + GFF2238 0.49 +0.4 34,234 + GFF2238 0.54 +0.5 34,252 + GFF2238 0.56 +0.4 34,444 + GFF2238 0.77 -0.7 34,466 - GFF2238 0.80 -0.8 34,643 + -0.5 34,643 + -0.4 34,646 + -0.4 34,647 - -1.4 34,647 - +1.7 34,655 + +0.7 34,655 + +0.2 34,656 - -0.6 34,726 - +0.1 34,748 + -0.8 34,750 + +0.9 34,756 + +0.4 34,817 - GFF2239 0.17 -0.3 34,841 - GFF2239 0.20 +1.5 34,957 + GFF2239 0.35 +0.1 34,977 + GFF2239 0.37 +1.5 35,032 + GFF2239 0.44 +1.6 35,033 - GFF2239 0.44 +0.8 35,048 - GFF2239 0.46 +0.2 35,082 - GFF2239 0.50 -0.7 35,082 - GFF2239 0.50 +0.1 35,083 + GFF2239 0.50 +0.1 35,084 - GFF2239 0.50 -0.3 35,084 - GFF2239 0.50 -0.1 35,137 + GFF2239 0.57 +1.1 35,209 - GFF2239 0.66 +0.8 35,297 - GFF2239 0.77 +0.1 35,530 + +0.4 35,611 - -0.5 35,643 + -0.3
Or see this region's nucleotide sequence