Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2167 and GFF2168 are separated by 188 nucleotides GFF2168 and GFF2169 overlap by 1 nucleotides GFF2169 and GFF2170 are separated by 35 nucleotides GFF2170 and GFF2171 are separated by 234 nucleotides
GFF2167 - Polyphosphate kinase (EC 2.7.4.1), at 14,381 to 16,447
GFF2167
GFF2168 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 16,636 to 17,274
GFF2168
GFF2169 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 17,274 to 18,311
GFF2169
GFF2170 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 18,347 to 18,490
GFF2170
GFF2171 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 18,725 to 19,351
GFF2171
Position (kb)
17
18
19 Strain fitness (log2 ratio)
-1
0
1
2
3 at 16.428 kb on + strand at 16.429 kb on - strand at 16.452 kb on - strand at 16.456 kb on - strand at 16.467 kb on + strand at 16.489 kb on - strand at 16.505 kb on - strand at 16.511 kb on + strand at 16.634 kb on + strand at 16.708 kb on + strand, within GFF2168 at 16.731 kb on + strand, within GFF2168 at 16.732 kb on - strand, within GFF2168 at 16.782 kb on + strand, within GFF2168 at 16.783 kb on - strand, within GFF2168 at 17.004 kb on + strand, within GFF2168 at 17.004 kb on + strand, within GFF2168 at 17.042 kb on + strand, within GFF2168 at 17.234 kb on - strand at 17.234 kb on - strand at 17.269 kb on - strand at 17.424 kb on - strand, within GFF2169 at 17.429 kb on - strand, within GFF2169 at 17.429 kb on - strand, within GFF2169 at 17.451 kb on - strand, within GFF2169 at 17.505 kb on - strand, within GFF2169 at 17.587 kb on - strand, within GFF2169 at 17.587 kb on - strand, within GFF2169 at 17.627 kb on - strand, within GFF2169 at 17.630 kb on + strand, within GFF2169 at 17.630 kb on + strand, within GFF2169 at 17.631 kb on - strand, within GFF2169 at 17.711 kb on + strand, within GFF2169 at 17.866 kb on + strand, within GFF2169 at 17.867 kb on - strand, within GFF2169 at 17.867 kb on - strand, within GFF2169 at 17.989 kb on + strand, within GFF2169 at 18.111 kb on + strand, within GFF2169 at 18.226 kb on - strand at 18.226 kb on - strand at 18.291 kb on - strand at 18.639 kb on + strand at 18.640 kb on - strand at 18.799 kb on + strand, within GFF2171 at 18.828 kb on + strand, within GFF2171 at 19.029 kb on - strand, within GFF2171 at 19.061 kb on + strand, within GFF2171 at 19.177 kb on + strand, within GFF2171 at 19.186 kb on + strand, within GFF2171 at 19.260 kb on - strand, within GFF2171 at 19.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 16,428 + +0.0 16,429 - +0.5 16,452 - -0.5 16,456 - +0.1 16,467 + +1.3 16,489 - +0.3 16,505 - +0.3 16,511 + -0.1 16,634 + -0.2 16,708 + GFF2168 0.11 +1.5 16,731 + GFF2168 0.15 -0.1 16,732 - GFF2168 0.15 -1.4 16,782 + GFF2168 0.23 -0.5 16,783 - GFF2168 0.23 -0.6 17,004 + GFF2168 0.58 +0.1 17,004 + GFF2168 0.58 +0.9 17,042 + GFF2168 0.64 +0.2 17,234 - +3.1 17,234 - -0.8 17,269 - +0.3 17,424 - GFF2169 0.14 +0.2 17,429 - GFF2169 0.15 +0.8 17,429 - GFF2169 0.15 +1.1 17,451 - GFF2169 0.17 +0.5 17,505 - GFF2169 0.22 +0.7 17,587 - GFF2169 0.30 +0.4 17,587 - GFF2169 0.30 +0.8 17,627 - GFF2169 0.34 +0.1 17,630 + GFF2169 0.34 +0.3 17,630 + GFF2169 0.34 +1.2 17,631 - GFF2169 0.34 +0.6 17,711 + GFF2169 0.42 -0.7 17,866 + GFF2169 0.57 -0.8 17,867 - GFF2169 0.57 +0.9 17,867 - GFF2169 0.57 +1.0 17,989 + GFF2169 0.69 +0.5 18,111 + GFF2169 0.81 +0.8 18,226 - +0.8 18,226 - +0.9 18,291 - +0.6 18,639 + +0.4 18,640 - +0.7 18,799 + GFF2171 0.12 +1.8 18,828 + GFF2171 0.16 +1.8 19,029 - GFF2171 0.48 +3.6 19,061 + GFF2171 0.54 +1.3 19,177 + GFF2171 0.72 +2.1 19,186 + GFF2171 0.74 +1.4 19,260 - GFF2171 0.85 -0.3 19,299 - +1.4
Or see this region's nucleotide sequence