Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2096 and GFF2097 are separated by 53 nucleotides GFF2097 and GFF2098 are separated by 72 nucleotides GFF2098 and GFF2099 are separated by 203 nucleotides
GFF2096 - Protein acetyltransferase, at 4,144 to 6,804
GFF2096
GFF2097 - hypothetical protein, at 6,858 to 7,538
GFF2097
GFF2098 - Thioredoxin 2 (EC 1.8.1.8), at 7,611 to 8,030
GFF2098
GFF2099 - hypothetical tRNA/rRNA methyltransferase yfiF [EC:2.1.1.-], at 8,234 to 9,271
GFF2099
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5.958 kb on + strand, within GFF2096 at 6.172 kb on + strand, within GFF2096 at 6.173 kb on - strand, within GFF2096 at 6.185 kb on + strand, within GFF2096 at 6.453 kb on + strand, within GFF2096 at 6.454 kb on - strand, within GFF2096 at 6.454 kb on - strand, within GFF2096 at 6.764 kb on + strand at 6.979 kb on - strand, within GFF2097 at 7.094 kb on + strand, within GFF2097 at 7.106 kb on + strand, within GFF2097 at 7.106 kb on + strand, within GFF2097 at 7.228 kb on + strand, within GFF2097 at 7.229 kb on - strand, within GFF2097 at 7.406 kb on + strand, within GFF2097 at 7.530 kb on - strand at 7.670 kb on + strand, within GFF2098 at 7.826 kb on + strand, within GFF2098 at 8.030 kb on + strand at 8.030 kb on + strand at 8.031 kb on - strand at 8.031 kb on - strand at 8.031 kb on - strand at 8.279 kb on + strand at 8.279 kb on + strand at 8.279 kb on + strand at 8.420 kb on - strand, within GFF2099 at 8.420 kb on - strand, within GFF2099 at 8.515 kb on + strand, within GFF2099 at 8.515 kb on + strand, within GFF2099
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 5,958 + GFF2096 0.68 -0.9 6,172 + GFF2096 0.76 -0.9 6,173 - GFF2096 0.76 +0.6 6,185 + GFF2096 0.77 -0.4 6,453 + GFF2096 0.87 +1.3 6,454 - GFF2096 0.87 +1.1 6,454 - GFF2096 0.87 -0.0 6,764 + +0.0 6,979 - GFF2097 0.18 +0.2 7,094 + GFF2097 0.35 -0.2 7,106 + GFF2097 0.36 +0.1 7,106 + GFF2097 0.36 -0.2 7,228 + GFF2097 0.54 -0.5 7,229 - GFF2097 0.54 -0.2 7,406 + GFF2097 0.80 +0.6 7,530 - -0.2 7,670 + GFF2098 0.14 +0.3 7,826 + GFF2098 0.51 -0.1 8,030 + +0.7 8,030 + +0.3 8,031 - -2.8 8,031 - +0.5 8,031 - +0.2 8,279 + -0.4 8,279 + +0.3 8,279 + +1.5 8,420 - GFF2099 0.18 +0.2 8,420 - GFF2099 0.18 -0.5 8,515 + GFF2099 0.27 -0.0 8,515 + GFF2099 0.27 -0.6
Or see this region's nucleotide sequence