Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2053 and GFF2054 are separated by 12 nucleotides GFF2054 and GFF2055 are separated by 130 nucleotides GFF2055 and GFF2056 are separated by 67 nucleotides
GFF2053 - hypothetical protein, at 18,607 to 18,987
GFF2053
GFF2054 - putative membrane carboxypeptidase, at 19,000 to 19,539
GFF2054
GFF2055 - Cysteine synthase (EC 2.5.1.47), at 19,670 to 20,581
GFF2055
GFF2056 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 20,649 to 21,746
GFF2056
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1 at 19.068 kb on - strand, within GFF2054 at 19.078 kb on + strand, within GFF2054 at 19.199 kb on - strand, within GFF2054 at 19.300 kb on + strand, within GFF2054 at 19.324 kb on + strand, within GFF2054 at 19.324 kb on + strand, within GFF2054 at 19.325 kb on - strand, within GFF2054 at 19.385 kb on + strand, within GFF2054 at 19.394 kb on + strand, within GFF2054 at 19.428 kb on - strand, within GFF2054 at 19.439 kb on - strand, within GFF2054 at 19.439 kb on - strand, within GFF2054 at 19.439 kb on - strand, within GFF2054 at 19.504 kb on + strand at 19.538 kb on - strand at 19.671 kb on + strand at 19.905 kb on - strand, within GFF2055 at 19.933 kb on + strand, within GFF2055 at 19.934 kb on - strand, within GFF2055 at 19.934 kb on - strand, within GFF2055 at 20.197 kb on + strand, within GFF2055 at 20.207 kb on + strand, within GFF2055 at 20.240 kb on - strand, within GFF2055 at 20.570 kb on + strand at 20.571 kb on - strand at 20.571 kb on - strand at 20.575 kb on + strand at 20.634 kb on - strand at 20.832 kb on - strand, within GFF2056 at 20.834 kb on - strand, within GFF2056 at 20.865 kb on + strand, within GFF2056 at 20.872 kb on - strand, within GFF2056 at 20.903 kb on + strand, within GFF2056 at 20.957 kb on + strand, within GFF2056 at 21.105 kb on - strand, within GFF2056 at 21.201 kb on + strand, within GFF2056 at 21.222 kb on + strand, within GFF2056 at 21.524 kb on + strand, within GFF2056 at 21.573 kb on - strand, within GFF2056
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 19,068 - GFF2054 0.13 +0.0 19,078 + GFF2054 0.14 +0.3 19,199 - GFF2054 0.37 -0.3 19,300 + GFF2054 0.56 +0.7 19,324 + GFF2054 0.60 +0.1 19,324 + GFF2054 0.60 +0.0 19,325 - GFF2054 0.60 -0.5 19,385 + GFF2054 0.71 -0.4 19,394 + GFF2054 0.73 +0.6 19,428 - GFF2054 0.79 -0.3 19,439 - GFF2054 0.81 -0.1 19,439 - GFF2054 0.81 -1.4 19,439 - GFF2054 0.81 -2.1 19,504 + -1.3 19,538 - -0.0 19,671 + -0.2 19,905 - GFF2055 0.26 +0.2 19,933 + GFF2055 0.29 -0.8 19,934 - GFF2055 0.29 -0.1 19,934 - GFF2055 0.29 +1.2 20,197 + GFF2055 0.58 -0.4 20,207 + GFF2055 0.59 -0.0 20,240 - GFF2055 0.62 -0.8 20,570 + -0.1 20,571 - +0.6 20,571 - +0.7 20,575 + -0.3 20,634 - +0.6 20,832 - GFF2056 0.17 +0.2 20,834 - GFF2056 0.17 +0.9 20,865 + GFF2056 0.20 +0.4 20,872 - GFF2056 0.20 -0.5 20,903 + GFF2056 0.23 +0.4 20,957 + GFF2056 0.28 +0.7 21,105 - GFF2056 0.42 +0.6 21,201 + GFF2056 0.50 -0.6 21,222 + GFF2056 0.52 +0.5 21,524 + GFF2056 0.80 +0.5 21,573 - GFF2056 0.84 -0.3
Or see this region's nucleotide sequence