Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1991 and GFF1992 are separated by 18 nucleotides GFF1992 and GFF1993 are separated by 66 nucleotides GFF1993 and GFF1994 are separated by 51 nucleotides
GFF1991 - Phosphatidylserine decarboxylase (EC 4.1.1.65), at 2,928 to 3,896
GFF1991
GFF1992 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 3,915 to 7,241
GFF1992
GFF1993 - FIG00639836: hypothetical protein, at 7,308 to 7,622
GFF1993
GFF1994 - Putative amino acid permease, at 7,674 to 9,176
GFF1994
Position (kb) 3
4
5
6
7
8 Strain fitness (log2 ratio) -1
0
1
2 at 3.811 kb on + strand at 3.878 kb on + strand at 3.894 kb on + strand at 3.894 kb on + strand at 4.130 kb on + strand at 4.130 kb on + strand at 4.150 kb on + strand at 4.264 kb on + strand, within GFF1992 at 4.272 kb on - strand, within GFF1992 at 4.371 kb on - strand, within GFF1992 at 4.575 kb on + strand, within GFF1992 at 4.700 kb on + strand, within GFF1992 at 4.796 kb on - strand, within GFF1992 at 4.956 kb on - strand, within GFF1992 at 4.979 kb on + strand, within GFF1992 at 5.002 kb on + strand, within GFF1992 at 5.003 kb on - strand, within GFF1992 at 5.281 kb on + strand, within GFF1992 at 5.286 kb on - strand, within GFF1992 at 5.341 kb on + strand, within GFF1992 at 5.342 kb on - strand, within GFF1992 at 5.542 kb on + strand, within GFF1992 at 5.543 kb on - strand, within GFF1992 at 5.617 kb on + strand, within GFF1992 at 5.713 kb on - strand, within GFF1992 at 5.748 kb on - strand, within GFF1992 at 6.054 kb on - strand, within GFF1992 at 6.058 kb on - strand, within GFF1992 at 6.058 kb on - strand, within GFF1992 at 6.234 kb on - strand, within GFF1992 at 6.301 kb on + strand, within GFF1992 at 6.402 kb on - strand, within GFF1992 at 6.402 kb on - strand, within GFF1992 at 6.437 kb on + strand, within GFF1992 at 6.461 kb on + strand, within GFF1992 at 6.493 kb on + strand, within GFF1992 at 6.494 kb on - strand, within GFF1992 at 6.613 kb on + strand, within GFF1992 at 6.614 kb on - strand, within GFF1992 at 6.807 kb on - strand, within GFF1992 at 6.816 kb on - strand, within GFF1992 at 6.892 kb on + strand, within GFF1992 at 7.043 kb on + strand at 7.044 kb on - strand at 7.044 kb on - strand at 7.070 kb on + strand at 7.070 kb on + strand at 7.071 kb on - strand at 7.134 kb on - strand at 7.134 kb on - strand at 7.134 kb on - strand at 7.134 kb on - strand at 7.178 kb on + strand at 7.281 kb on + strand at 7.282 kb on - strand at 7.299 kb on + strand at 7.304 kb on + strand at 7.324 kb on + strand at 7.359 kb on + strand, within GFF1993 at 7.396 kb on - strand, within GFF1993 at 7.396 kb on - strand, within GFF1993 at 7.405 kb on + strand, within GFF1993 at 7.625 kb on + strand at 7.640 kb on + strand at 7.654 kb on + strand at 7.717 kb on + strand at 7.726 kb on + strand at 7.823 kb on + strand at 8.219 kb on + strand, within GFF1994
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 3,811 + +0.2 3,878 + -1.0 3,894 + +0.4 3,894 + -0.7 4,130 + +0.5 4,130 + -0.1 4,150 + +0.6 4,264 + GFF1992 0.10 -0.2 4,272 - GFF1992 0.11 +0.7 4,371 - GFF1992 0.14 +1.0 4,575 + GFF1992 0.20 +0.8 4,700 + GFF1992 0.24 +0.5 4,796 - GFF1992 0.26 -0.6 4,956 - GFF1992 0.31 +0.0 4,979 + GFF1992 0.32 +0.1 5,002 + GFF1992 0.33 -0.4 5,003 - GFF1992 0.33 +0.8 5,281 + GFF1992 0.41 +0.5 5,286 - GFF1992 0.41 -0.1 5,341 + GFF1992 0.43 -0.2 5,342 - GFF1992 0.43 +1.5 5,542 + GFF1992 0.49 -0.2 5,543 - GFF1992 0.49 +1.0 5,617 + GFF1992 0.51 +1.2 5,713 - GFF1992 0.54 +0.3 5,748 - GFF1992 0.55 -0.7 6,054 - GFF1992 0.64 -0.3 6,058 - GFF1992 0.64 -0.2 6,058 - GFF1992 0.64 -0.2 6,234 - GFF1992 0.70 +1.9 6,301 + GFF1992 0.72 +0.8 6,402 - GFF1992 0.75 -0.5 6,402 - GFF1992 0.75 -0.3 6,437 + GFF1992 0.76 +1.1 6,461 + GFF1992 0.77 -0.5 6,493 + GFF1992 0.77 -0.3 6,494 - GFF1992 0.78 +0.4 6,613 + GFF1992 0.81 -0.1 6,614 - GFF1992 0.81 +0.5 6,807 - GFF1992 0.87 -0.1 6,816 - GFF1992 0.87 +0.6 6,892 + GFF1992 0.89 -0.2 7,043 + +0.5 7,044 - +0.2 7,044 - +0.2 7,070 + +0.1 7,070 + +0.7 7,071 - +0.1 7,134 - +1.1 7,134 - -0.1 7,134 - -1.0 7,134 - -0.2 7,178 + +0.3 7,281 + +0.6 7,282 - +0.5 7,299 + +0.1 7,304 + -0.3 7,324 + +0.2 7,359 + GFF1993 0.16 +0.1 7,396 - GFF1993 0.28 -0.2 7,396 - GFF1993 0.28 -0.3 7,405 + GFF1993 0.31 +0.1 7,625 + +0.5 7,640 + -0.7 7,654 + +0.7 7,717 + +0.0 7,726 + -0.0 7,823 + -0.2 8,219 + GFF1994 0.36 +1.7
Or see this region's nucleotide sequence