Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1975 and GFF1976 are separated by 113 nucleotides GFF1976 and GFF1977 are separated by 60 nucleotides GFF1977 and GFF1978 are separated by 405 nucleotides
GFF1975 - Putative ion-channel protein, at 168,610 to 169,608
GFF1975
GFF1976 - FIG00639029: hypothetical protein, at 169,722 to 170,048
GFF1976
GFF1977 - Manganese transport protein MntH, at 170,109 to 171,287
GFF1977
GFF1978 - Nucleoside permease NupC, at 171,693 to 172,895
GFF1978
Position (kb) 170
171
172 Strain fitness (log2 ratio) -1
0
1
2
3 at 169.114 kb on - strand, within GFF1975 at 169.117 kb on + strand, within GFF1975 at 169.143 kb on + strand, within GFF1975 at 169.192 kb on - strand, within GFF1975 at 169.195 kb on + strand, within GFF1975 at 169.306 kb on + strand, within GFF1975 at 169.307 kb on - strand, within GFF1975 at 169.374 kb on + strand, within GFF1975 at 169.721 kb on + strand at 169.848 kb on + strand, within GFF1976 at 169.848 kb on + strand, within GFF1976 at 169.955 kb on + strand, within GFF1976 at 170.047 kb on - strand at 170.148 kb on - strand at 170.176 kb on - strand at 170.229 kb on - strand, within GFF1977 at 170.360 kb on + strand, within GFF1977 at 170.437 kb on - strand, within GFF1977 at 170.472 kb on - strand, within GFF1977 at 170.503 kb on - strand, within GFF1977 at 170.524 kb on + strand, within GFF1977 at 170.630 kb on + strand, within GFF1977 at 170.630 kb on + strand, within GFF1977 at 170.656 kb on + strand, within GFF1977 at 171.227 kb on + strand at 171.441 kb on - strand at 171.509 kb on - strand at 171.604 kb on + strand at 171.609 kb on + strand at 171.786 kb on + strand at 171.799 kb on + strand at 171.815 kb on - strand, within GFF1978 at 172.191 kb on + strand, within GFF1978 at 172.191 kb on + strand, within GFF1978 at 172.192 kb on - strand, within GFF1978 at 172.247 kb on + strand, within GFF1978 at 172.248 kb on - strand, within GFF1978 at 172.269 kb on + strand, within GFF1978 at 172.270 kb on - strand, within GFF1978 at 172.270 kb on - strand, within GFF1978
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 169,114 - GFF1975 0.50 +0.2 169,117 + GFF1975 0.51 -1.3 169,143 + GFF1975 0.53 +0.1 169,192 - GFF1975 0.58 +0.7 169,195 + GFF1975 0.59 +0.4 169,306 + GFF1975 0.70 -0.3 169,307 - GFF1975 0.70 -0.4 169,374 + GFF1975 0.76 -0.4 169,721 + +0.6 169,848 + GFF1976 0.39 -0.3 169,848 + GFF1976 0.39 +0.1 169,955 + GFF1976 0.71 +0.5 170,047 - -1.5 170,148 - -0.6 170,176 - -0.2 170,229 - GFF1977 0.10 -0.2 170,360 + GFF1977 0.21 -0.5 170,437 - GFF1977 0.28 +3.2 170,472 - GFF1977 0.31 +0.6 170,503 - GFF1977 0.33 +0.8 170,524 + GFF1977 0.35 -0.1 170,630 + GFF1977 0.44 +0.0 170,630 + GFF1977 0.44 -0.8 170,656 + GFF1977 0.46 +0.4 171,227 + -0.6 171,441 - +0.0 171,509 - -0.0 171,604 + -0.1 171,609 + -0.4 171,786 + +1.0 171,799 + +1.4 171,815 - GFF1978 0.10 +1.5 172,191 + GFF1978 0.41 +0.5 172,191 + GFF1978 0.41 +0.5 172,192 - GFF1978 0.41 +0.2 172,247 + GFF1978 0.46 +1.7 172,248 - GFF1978 0.46 +0.6 172,269 + GFF1978 0.48 +1.0 172,270 - GFF1978 0.48 +0.6 172,270 - GFF1978 0.48 +1.3
Or see this region's nucleotide sequence