Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1841

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1839 and GFF1840 overlap by 1 nucleotidesGFF1840 and GFF1841 are separated by 1 nucleotidesGFF1841 and GFF1842 are separated by 161 nucleotides GFF1839 - Ribonucleotide reductase of class Ia (aerobic), beta subunit (EC 1.17.4.1), at 30,191 to 31,321 GFF1839 GFF1840 - Ferredoxin, at 31,321 to 31,575 GFF1840 GFF1841 - Putative permease, at 31,577 to 32,767 GFF1841 GFF1842 - LysR family transcriptional regulator STM2281, at 32,929 to 33,807 GFF1842 Position (kb) 31 32 33Strain fitness (log2 ratio) -2 -1 0 1 2at 31.638 kb on + strandat 31.638 kb on + strandat 31.656 kb on - strandat 31.688 kb on - strandat 31.724 kb on - strand, within GFF1841at 31.954 kb on - strand, within GFF1841at 31.970 kb on - strand, within GFF1841at 32.001 kb on - strand, within GFF1841at 32.071 kb on - strand, within GFF1841at 32.425 kb on + strand, within GFF1841at 32.426 kb on - strand, within GFF1841at 32.824 kb on + strandat 32.849 kb on - strandat 33.004 kb on + strandat 33.005 kb on - strandat 33.068 kb on + strand, within GFF1842at 33.319 kb on - strand, within GFF1842at 33.352 kb on - strand, within GFF1842at 33.387 kb on + strand, within GFF1842

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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31,638 + -0.7
31,638 + -0.3
31,656 - +0.1
31,688 - -1.8
31,724 - GFF1841 0.12 -0.5
31,954 - GFF1841 0.32 -0.3
31,970 - GFF1841 0.33 +0.1
32,001 - GFF1841 0.36 -0.6
32,071 - GFF1841 0.41 -1.0
32,425 + GFF1841 0.71 +0.8
32,426 - GFF1841 0.71 -0.6
32,824 + +2.6
32,849 - +0.5
33,004 + +0.3
33,005 - +1.2
33,068 + GFF1842 0.16 -1.5
33,319 - GFF1842 0.44 +0.4
33,352 - GFF1842 0.48 +1.1
33,387 + GFF1842 0.52 +1.9

Or see this region's nucleotide sequence