Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF177 and GFF178 are separated by 10 nucleotides GFF178 and GFF179 are separated by 140 nucleotides GFF179 and GFF180 are separated by 5 nucleotides
GFF177 - Ribulokinase (EC 2.7.1.16), at 167,791 to 169,500
GFF177
GFF178 - L-arabinose isomerase (EC 5.3.1.4), at 169,511 to 171,013
GFF178
GFF179 - L-ribulose-5-phosphate 4-epimerase (EC 5.1.3.4), at 171,154 to 171,849
GFF179
GFF180 - FIG01045455: hypothetical protein, at 171,855 to 172,274
GFF180
Position (kb) 169
170
171
172 Strain fitness (log2 ratio) -2
-1
0
1
2 at 168.912 kb on - strand, within GFF177 at 169.215 kb on - strand, within GFF177 at 169.343 kb on + strand at 169.498 kb on + strand at 169.529 kb on + strand at 169.529 kb on + strand at 169.530 kb on - strand at 169.565 kb on + strand at 169.565 kb on + strand at 169.716 kb on - strand, within GFF178 at 169.792 kb on + strand, within GFF178 at 169.810 kb on - strand, within GFF178 at 169.870 kb on + strand, within GFF178 at 169.870 kb on + strand, within GFF178 at 169.871 kb on - strand, within GFF178 at 169.915 kb on + strand, within GFF178 at 169.970 kb on - strand, within GFF178 at 170.063 kb on - strand, within GFF178 at 170.093 kb on - strand, within GFF178 at 170.220 kb on - strand, within GFF178 at 170.342 kb on - strand, within GFF178 at 170.914 kb on - strand at 171.158 kb on + strand at 171.225 kb on + strand, within GFF179 at 171.297 kb on + strand, within GFF179 at 171.404 kb on - strand, within GFF179 at 171.434 kb on + strand, within GFF179 at 171.737 kb on - strand, within GFF179 at 171.835 kb on + strand at 171.836 kb on - strand at 171.836 kb on - strand at 171.856 kb on + strand at 171.949 kb on + strand, within GFF180
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 168,912 - GFF177 0.66 +0.5 169,215 - GFF177 0.83 +0.1 169,343 + +0.3 169,498 + -0.2 169,529 + +1.5 169,529 + -0.4 169,530 - +0.1 169,565 + +1.1 169,565 + +0.1 169,716 - GFF178 0.14 +0.5 169,792 + GFF178 0.19 -1.2 169,810 - GFF178 0.20 -0.2 169,870 + GFF178 0.24 -0.2 169,870 + GFF178 0.24 -1.0 169,871 - GFF178 0.24 +0.5 169,915 + GFF178 0.27 +0.7 169,970 - GFF178 0.31 -1.1 170,063 - GFF178 0.37 +0.3 170,093 - GFF178 0.39 -0.0 170,220 - GFF178 0.47 -2.2 170,342 - GFF178 0.55 +0.4 170,914 - +0.4 171,158 + -0.4 171,225 + GFF179 0.10 +2.4 171,297 + GFF179 0.21 -0.3 171,404 - GFF179 0.36 -0.4 171,434 + GFF179 0.40 -0.9 171,737 - GFF179 0.84 +0.8 171,835 + +0.5 171,836 - +0.1 171,836 - +0.4 171,856 + -0.3 171,949 + GFF180 0.22 -0.2
Or see this region's nucleotide sequence