Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1598 and GFF1599 overlap by 4 nucleotides GFF1599 and GFF1600 are separated by 82 nucleotides GFF1600 and GFF1601 are separated by 126 nucleotides GFF1601 and GFF1602 are separated by 193 nucleotides
GFF1598 - Putative membrane protein, at 56,750 to 57,175
GFF1598
GFF1599 - Putative phosphatase YqaB, at 57,172 to 57,738
GFF1599
GFF1600 - HAD-superfamily hydrolase, subfamily IA, at 57,821 to 57,982
GFF1600
GFF1601 - tRNA-Arg-ACG, at 58,109 to 58,185
GFF1601
GFF1602 - tRNA-Arg-ACG, at 58,379 to 58,455
GFF1602
Position (kb) 57
58 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 56.909 kb on + strand, within GFF1598 at 56.910 kb on - strand, within GFF1598 at 56.910 kb on - strand, within GFF1598 at 57.004 kb on + strand, within GFF1598 at 57.005 kb on - strand, within GFF1598 at 57.005 kb on - strand, within GFF1598 at 57.005 kb on - strand, within GFF1598 at 57.023 kb on - strand, within GFF1598 at 57.047 kb on + strand, within GFF1598 at 57.082 kb on + strand, within GFF1598 at 57.083 kb on - strand, within GFF1598 at 57.178 kb on + strand at 57.556 kb on - strand, within GFF1599 at 57.638 kb on + strand, within GFF1599 at 57.706 kb on - strand at 57.726 kb on - strand at 57.735 kb on - strand at 57.803 kb on - strand at 57.816 kb on + strand at 57.817 kb on - strand at 57.817 kb on - strand at 57.854 kb on + strand, within GFF1600 at 57.940 kb on + strand, within GFF1600 at 58.063 kb on + strand at 58.065 kb on + strand at 58.070 kb on - strand at 58.237 kb on - strand at 58.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 56,909 + GFF1598 0.37 -0.0 56,910 - GFF1598 0.38 +0.7 56,910 - GFF1598 0.38 -0.5 57,004 + GFF1598 0.60 +0.2 57,005 - GFF1598 0.60 -0.1 57,005 - GFF1598 0.60 -0.1 57,005 - GFF1598 0.60 -0.4 57,023 - GFF1598 0.64 +0.4 57,047 + GFF1598 0.70 -0.3 57,082 + GFF1598 0.78 -0.9 57,083 - GFF1598 0.78 +0.1 57,178 + +0.5 57,556 - GFF1599 0.68 -0.1 57,638 + GFF1599 0.82 +0.8 57,706 - +0.0 57,726 - +0.4 57,735 - +0.2 57,803 - -0.0 57,816 + +0.0 57,817 - +0.1 57,817 - -0.6 57,854 + GFF1600 0.20 +0.0 57,940 + GFF1600 0.73 -0.8 58,063 + -0.3 58,065 + -0.1 58,070 - -1.9 58,237 - +2.9 58,344 + -0.4
Or see this region's nucleotide sequence