Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1597

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1595 and GFF1596 are separated by 57 nucleotidesGFF1596 and GFF1597 are separated by 149 nucleotidesGFF1597 and GFF1598 are separated by 76 nucleotidesGFF1598 and GFF1599 overlap by 4 nucleotides GFF1595 - hypothetical protein, at 54,251 to 54,394 GFF1595 GFF1596 - S-ribosylhomocysteine lyase (EC 4.4.1.21) / Autoinducer-2 production protein LuxS, at 54,452 to 54,967 GFF1596 GFF1597 - Glutamate--cysteine ligase (EC 6.3.2.2), at 55,117 to 56,673 GFF1597 GFF1598 - Putative membrane protein, at 56,750 to 57,175 GFF1598 GFF1599 - Putative phosphatase YqaB, at 57,172 to 57,738 GFF1599 Position (kb) 55 56 57Strain fitness (log2 ratio) -2 -1 0 1 2 3at 54.532 kb on + strand, within GFF1596at 54.576 kb on + strand, within GFF1596at 54.705 kb on + strand, within GFF1596at 54.706 kb on - strand, within GFF1596at 54.806 kb on - strand, within GFF1596at 54.953 kb on - strandat 54.953 kb on - strandat 54.953 kb on - strandat 54.957 kb on - strandat 54.957 kb on - strandat 54.983 kb on - strandat 55.186 kb on + strandat 55.312 kb on - strand, within GFF1597at 55.318 kb on + strand, within GFF1597at 55.518 kb on + strand, within GFF1597at 55.518 kb on + strand, within GFF1597at 55.558 kb on - strand, within GFF1597at 55.561 kb on + strand, within GFF1597at 55.561 kb on + strand, within GFF1597at 55.561 kb on + strand, within GFF1597at 55.561 kb on + strand, within GFF1597at 55.562 kb on - strand, within GFF1597at 55.562 kb on - strand, within GFF1597at 55.601 kb on - strand, within GFF1597at 55.671 kb on + strand, within GFF1597at 55.697 kb on + strand, within GFF1597at 55.873 kb on - strand, within GFF1597at 55.898 kb on + strand, within GFF1597at 55.915 kb on - strand, within GFF1597at 55.953 kb on - strand, within GFF1597at 55.997 kb on + strand, within GFF1597at 55.998 kb on - strand, within GFF1597at 55.998 kb on - strand, within GFF1597at 55.998 kb on - strand, within GFF1597at 55.998 kb on - strand, within GFF1597at 55.998 kb on - strand, within GFF1597at 56.088 kb on - strand, within GFF1597at 56.227 kb on + strand, within GFF1597at 56.333 kb on - strand, within GFF1597at 56.355 kb on - strand, within GFF1597at 56.355 kb on - strand, within GFF1597at 56.364 kb on + strand, within GFF1597at 56.365 kb on - strand, within GFF1597at 56.407 kb on - strand, within GFF1597at 56.427 kb on + strand, within GFF1597at 56.428 kb on - strand, within GFF1597at 56.440 kb on - strand, within GFF1597at 56.440 kb on - strand, within GFF1597at 56.440 kb on - strand, within GFF1597at 56.530 kb on + strandat 56.706 kb on - strandat 56.711 kb on + strandat 56.712 kb on - strandat 56.718 kb on - strandat 56.730 kb on - strandat 56.748 kb on + strandat 56.909 kb on + strand, within GFF1598at 56.910 kb on - strand, within GFF1598at 56.910 kb on - strand, within GFF1598at 57.004 kb on + strand, within GFF1598at 57.005 kb on - strand, within GFF1598at 57.005 kb on - strand, within GFF1598at 57.005 kb on - strand, within GFF1598at 57.023 kb on - strand, within GFF1598at 57.047 kb on + strand, within GFF1598at 57.082 kb on + strand, within GFF1598at 57.083 kb on - strand, within GFF1598at 57.178 kb on + strandat 57.556 kb on - strand, within GFF1599at 57.638 kb on + strand, within GFF1599

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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54,532 + GFF1596 0.16 +0.2
54,576 + GFF1596 0.24 +2.3
54,705 + GFF1596 0.49 -0.6
54,706 - GFF1596 0.49 -0.3
54,806 - GFF1596 0.69 -1.8
54,953 - +2.8
54,953 - +0.9
54,953 - -0.1
54,957 - -1.3
54,957 - -0.7
54,983 - -1.6
55,186 + +3.5
55,312 - GFF1597 0.13 +1.8
55,318 + GFF1597 0.13 +1.0
55,518 + GFF1597 0.26 +1.5
55,518 + GFF1597 0.26 +1.0
55,558 - GFF1597 0.28 +1.3
55,561 + GFF1597 0.29 +2.0
55,561 + GFF1597 0.29 +2.1
55,561 + GFF1597 0.29 +2.1
55,561 + GFF1597 0.29 +1.3
55,562 - GFF1597 0.29 +2.3
55,562 - GFF1597 0.29 +1.0
55,601 - GFF1597 0.31 +1.9
55,671 + GFF1597 0.36 +1.9
55,697 + GFF1597 0.37 +2.3
55,873 - GFF1597 0.49 +2.3
55,898 + GFF1597 0.50 +0.9
55,915 - GFF1597 0.51 +1.1
55,953 - GFF1597 0.54 +1.7
55,997 + GFF1597 0.57 +3.6
55,998 - GFF1597 0.57 +0.5
55,998 - GFF1597 0.57 +2.0
55,998 - GFF1597 0.57 +1.7
55,998 - GFF1597 0.57 +1.9
55,998 - GFF1597 0.57 +1.8
56,088 - GFF1597 0.62 +1.3
56,227 + GFF1597 0.71 +2.0
56,333 - GFF1597 0.78 +1.5
56,355 - GFF1597 0.80 +1.7
56,355 - GFF1597 0.80 +0.8
56,364 + GFF1597 0.80 +1.8
56,365 - GFF1597 0.80 +1.9
56,407 - GFF1597 0.83 +2.2
56,427 + GFF1597 0.84 +2.2
56,428 - GFF1597 0.84 +1.7
56,440 - GFF1597 0.85 +2.1
56,440 - GFF1597 0.85 +1.6
56,440 - GFF1597 0.85 +1.8
56,530 + +1.9
56,706 - +0.7
56,711 + +1.4
56,712 - +1.3
56,718 - -0.0
56,730 - +0.0
56,748 + +1.1
56,909 + GFF1598 0.37 -0.0
56,910 - GFF1598 0.38 +0.7
56,910 - GFF1598 0.38 -0.5
57,004 + GFF1598 0.60 +0.2
57,005 - GFF1598 0.60 -0.1
57,005 - GFF1598 0.60 -0.1
57,005 - GFF1598 0.60 -0.4
57,023 - GFF1598 0.64 +0.4
57,047 + GFF1598 0.70 -0.3
57,082 + GFF1598 0.78 -0.9
57,083 - GFF1598 0.78 +0.1
57,178 + +0.5
57,556 - GFF1599 0.68 -0.1
57,638 + GFF1599 0.82 +0.8

Or see this region's nucleotide sequence