Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1577 and GFF1578 are separated by 34 nucleotides GFF1578 and GFF1579 are separated by 161 nucleotides GFF1579 and GFF1580 are separated by 83 nucleotides GFF1580 and GFF1581 are separated by 85 nucleotides GFF1581 and GFF1582 are separated by 14 nucleotides GFF1582 and GFF1583 are separated by 98 nucleotides
GFF1577 - Putative inner membrane protein, at 36,507 to 36,956
GFF1577
GFF1578 - FIG01219827: hypothetical protein, at 36,991 to 37,341
GFF1578
GFF1579 - FIG00638140: hypothetical protein, at 37,503 to 37,829
GFF1579
GFF1580 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 37,913 to 39,247
GFF1580
GFF1581 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 39,333 to 39,767
GFF1581
GFF1582 - FIG01046421: hypothetical protein, at 39,782 to 39,940
GFF1582
GFF1583 - Glutaredoxin-like protein NrdH, required for reduction of Ribonucleotide reductase class Ib, at 40,039 to 40,284
GFF1583
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -1
0
1
2
3 at 37.101 kb on - strand, within GFF1578 at 37.167 kb on + strand, within GFF1578 at 37.168 kb on - strand, within GFF1578 at 37.239 kb on - strand, within GFF1578 at 37.239 kb on - strand, within GFF1578 at 37.262 kb on + strand, within GFF1578 at 37.262 kb on + strand, within GFF1578 at 37.374 kb on - strand at 37.466 kb on + strand at 37.478 kb on + strand at 37.502 kb on + strand at 37.646 kb on + strand, within GFF1579 at 37.646 kb on + strand, within GFF1579 at 37.695 kb on - strand, within GFF1579 at 37.855 kb on + strand at 37.855 kb on + strand at 37.856 kb on - strand at 37.969 kb on + strand at 38.030 kb on + strand at 38.188 kb on - strand, within GFF1580 at 38.188 kb on - strand, within GFF1580 at 38.270 kb on + strand, within GFF1580 at 38.276 kb on - strand, within GFF1580 at 38.348 kb on + strand, within GFF1580 at 38.349 kb on - strand, within GFF1580 at 38.670 kb on - strand, within GFF1580 at 38.737 kb on + strand, within GFF1580 at 38.737 kb on + strand, within GFF1580 at 38.737 kb on + strand, within GFF1580 at 38.738 kb on - strand, within GFF1580 at 38.755 kb on + strand, within GFF1580 at 38.756 kb on - strand, within GFF1580 at 38.783 kb on + strand, within GFF1580 at 39.108 kb on + strand, within GFF1580 at 39.109 kb on - strand, within GFF1580 at 39.271 kb on + strand at 39.302 kb on + strand at 39.303 kb on - strand at 39.303 kb on - strand at 39.361 kb on - strand at 39.381 kb on + strand, within GFF1581 at 39.540 kb on - strand, within GFF1581 at 39.544 kb on + strand, within GFF1581 at 39.544 kb on + strand, within GFF1581 at 39.660 kb on + strand, within GFF1581
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 37,101 - GFF1578 0.31 +0.8 37,167 + GFF1578 0.50 -0.8 37,168 - GFF1578 0.50 -0.2 37,239 - GFF1578 0.71 +1.2 37,239 - GFF1578 0.71 -1.5 37,262 + GFF1578 0.77 -0.7 37,262 + GFF1578 0.77 -0.4 37,374 - +0.3 37,466 + -1.1 37,478 + +0.3 37,502 + -0.3 37,646 + GFF1579 0.44 +0.3 37,646 + GFF1579 0.44 +0.3 37,695 - GFF1579 0.59 -0.4 37,855 + +0.3 37,855 + +3.0 37,856 - -0.2 37,969 + -0.3 38,030 + +1.3 38,188 - GFF1580 0.21 +0.4 38,188 - GFF1580 0.21 +1.2 38,270 + GFF1580 0.27 +0.5 38,276 - GFF1580 0.27 -0.1 38,348 + GFF1580 0.33 -0.4 38,349 - GFF1580 0.33 +0.0 38,670 - GFF1580 0.57 +1.9 38,737 + GFF1580 0.62 +0.6 38,737 + GFF1580 0.62 +0.5 38,737 + GFF1580 0.62 +0.3 38,738 - GFF1580 0.62 +0.2 38,755 + GFF1580 0.63 +0.8 38,756 - GFF1580 0.63 -0.2 38,783 + GFF1580 0.65 +1.6 39,108 + GFF1580 0.90 +0.1 39,109 - GFF1580 0.90 +0.8 39,271 + -0.3 39,302 + -0.2 39,303 - +0.1 39,303 - +0.1 39,361 - -1.7 39,381 + GFF1581 0.11 +0.3 39,540 - GFF1581 0.48 -0.3 39,544 + GFF1581 0.49 +0.6 39,544 + GFF1581 0.49 +0.7 39,660 + GFF1581 0.75 +0.3
Or see this region's nucleotide sequence