Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1424 and GFF1425 are separated by 128 nucleotides GFF1425 and GFF1426 are separated by 2 nucleotides GFF1426 and GFF1427 are separated by 0 nucleotides GFF1427 and GFF1428 overlap by 14 nucleotides
GFF1424 - NMN phosphatase (EC 3.1.3.5); Class B acid phosphatase precursor (EC 3.1.3.2), at 70,814 to 71,527
GFF1424
GFF1425 - FIG004064: hypothetical protein, at 71,656 to 72,072
GFF1425
GFF1426 - Protein yjbR, at 72,075 to 72,431
GFF1426
GFF1427 - Putative inner membrane protein, at 72,432 to 72,770
GFF1427
GFF1428 - Putative outer membrane lipoprotein, at 72,757 to 73,200
GFF1428
Position (kb) 71
72
73 Strain fitness (log2 ratio) -1
0
1
2
3 at 70.672 kb on - strand at 70.672 kb on - strand at 70.672 kb on - strand at 70.672 kb on - strand at 70.887 kb on + strand, within GFF1424 at 71.097 kb on - strand, within GFF1424 at 71.214 kb on - strand, within GFF1424 at 71.214 kb on - strand, within GFF1424 at 71.214 kb on - strand, within GFF1424 at 71.401 kb on + strand, within GFF1424 at 71.402 kb on - strand, within GFF1424 at 71.620 kb on - strand at 71.655 kb on + strand at 71.656 kb on - strand at 71.656 kb on - strand at 71.656 kb on - strand at 71.662 kb on + strand at 71.676 kb on + strand at 71.676 kb on + strand at 71.783 kb on + strand, within GFF1425 at 71.788 kb on - strand, within GFF1425 at 71.896 kb on + strand, within GFF1425 at 71.896 kb on + strand, within GFF1425 at 71.925 kb on + strand, within GFF1425 at 71.928 kb on + strand, within GFF1425 at 72.003 kb on + strand, within GFF1425 at 72.003 kb on + strand, within GFF1425 at 72.004 kb on - strand, within GFF1425 at 72.059 kb on + strand at 72.190 kb on + strand, within GFF1426 at 72.190 kb on + strand, within GFF1426 at 72.190 kb on + strand, within GFF1426 at 72.253 kb on + strand, within GFF1426 at 72.274 kb on + strand, within GFF1426 at 72.373 kb on - strand, within GFF1426 at 72.433 kb on + strand at 72.485 kb on + strand, within GFF1427 at 72.522 kb on - strand, within GFF1427 at 72.532 kb on - strand, within GFF1427 at 72.532 kb on - strand, within GFF1427 at 72.566 kb on + strand, within GFF1427 at 72.674 kb on + strand, within GFF1427 at 72.674 kb on + strand, within GFF1427 at 72.674 kb on + strand, within GFF1427 at 72.675 kb on - strand, within GFF1427 at 72.676 kb on + strand, within GFF1427 at 72.676 kb on + strand, within GFF1427 at 72.745 kb on + strand at 72.750 kb on + strand at 72.750 kb on + strand at 72.751 kb on - strand at 72.758 kb on + strand at 72.820 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.876 kb on - strand, within GFF1428 at 72.876 kb on - strand, within GFF1428 at 72.879 kb on + strand, within GFF1428 at 72.923 kb on + strand, within GFF1428
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 70,672 - -0.5 70,672 - -0.5 70,672 - +2.4 70,672 - -1.1 70,887 + GFF1424 0.10 -0.0 71,097 - GFF1424 0.40 -0.2 71,214 - GFF1424 0.56 -0.3 71,214 - GFF1424 0.56 +0.6 71,214 - GFF1424 0.56 +0.0 71,401 + GFF1424 0.82 -0.2 71,402 - GFF1424 0.82 +0.9 71,620 - -0.3 71,655 + +1.2 71,656 - -0.3 71,656 - -0.1 71,656 - -0.6 71,662 + +0.5 71,676 + -0.2 71,676 + +0.9 71,783 + GFF1425 0.30 +1.6 71,788 - GFF1425 0.32 +0.3 71,896 + GFF1425 0.58 +3.1 71,896 + GFF1425 0.58 +0.0 71,925 + GFF1425 0.65 -0.0 71,928 + GFF1425 0.65 +0.3 72,003 + GFF1425 0.83 +0.3 72,003 + GFF1425 0.83 +0.6 72,004 - GFF1425 0.83 -0.7 72,059 + +0.1 72,190 + GFF1426 0.32 -1.3 72,190 + GFF1426 0.32 +0.2 72,190 + GFF1426 0.32 +0.2 72,253 + GFF1426 0.50 +1.0 72,274 + GFF1426 0.56 -0.1 72,373 - GFF1426 0.83 +0.6 72,433 + +0.2 72,485 + GFF1427 0.16 +0.4 72,522 - GFF1427 0.27 +1.1 72,532 - GFF1427 0.29 -0.5 72,532 - GFF1427 0.29 -0.1 72,566 + GFF1427 0.40 +0.0 72,674 + GFF1427 0.71 +0.6 72,674 + GFF1427 0.71 +0.7 72,674 + GFF1427 0.71 -0.5 72,675 - GFF1427 0.72 -0.0 72,676 + GFF1427 0.72 -0.6 72,676 + GFF1427 0.72 +0.2 72,745 + -0.1 72,750 + -0.4 72,750 + -0.2 72,751 - -0.3 72,758 + -0.6 72,820 + GFF1428 0.14 -1.6 72,875 + GFF1428 0.27 +0.2 72,875 + GFF1428 0.27 -0.8 72,875 + GFF1428 0.27 +0.9 72,875 + GFF1428 0.27 +0.7 72,876 - GFF1428 0.27 -0.1 72,876 - GFF1428 0.27 -0.2 72,879 + GFF1428 0.27 -1.0 72,923 + GFF1428 0.37 +0.6
Or see this region's nucleotide sequence