Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1302

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1301 and GFF1302 overlap by 8 nucleotidesGFF1302 and GFF1303 are separated by 177 nucleotides GFF1301 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 4,916 to 8,461 GFF1301 GFF1302 - Protease III precursor (EC 3.4.24.55), at 8,454 to 11,342 GFF1302 GFF1303 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 11,520 to 14,891 GFF1303 Position (kb) 8 9 10 11 12Strain fitness (log2 ratio) -1 0 1 2 3at 8.539 kb on + strandat 8.540 kb on - strandat 8.540 kb on - strandat 8.716 kb on - strandat 9.006 kb on + strand, within GFF1302at 9.006 kb on + strand, within GFF1302at 9.007 kb on - strand, within GFF1302at 9.087 kb on - strand, within GFF1302at 9.087 kb on - strand, within GFF1302at 9.222 kb on - strand, within GFF1302at 9.269 kb on + strand, within GFF1302at 9.270 kb on - strand, within GFF1302at 9.338 kb on - strand, within GFF1302at 9.338 kb on - strand, within GFF1302at 9.369 kb on - strand, within GFF1302at 9.369 kb on - strand, within GFF1302at 9.467 kb on + strand, within GFF1302at 9.515 kb on + strand, within GFF1302at 9.516 kb on - strand, within GFF1302at 9.558 kb on - strand, within GFF1302at 9.622 kb on + strand, within GFF1302at 9.622 kb on + strand, within GFF1302at 9.721 kb on + strand, within GFF1302at 9.944 kb on + strand, within GFF1302at 10.112 kb on - strand, within GFF1302at 10.181 kb on - strand, within GFF1302at 10.188 kb on - strand, within GFF1302at 10.337 kb on + strand, within GFF1302at 10.401 kb on + strand, within GFF1302at 10.405 kb on + strand, within GFF1302at 10.482 kb on - strand, within GFF1302at 10.572 kb on - strand, within GFF1302at 10.629 kb on - strand, within GFF1302at 10.658 kb on + strand, within GFF1302at 10.917 kb on + strand, within GFF1302at 11.010 kb on - strand, within GFF1302at 11.010 kb on - strand, within GFF1302at 11.066 kb on + strandat 11.066 kb on + strandat 11.066 kb on + strandat 11.067 kb on - strandat 11.244 kb on - strandat 11.359 kb on + strandat 11.392 kb on + strandat 11.420 kb on - strandat 11.422 kb on + strandat 11.423 kb on - strandat 11.456 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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8,539 + -1.3
8,540 - +1.9
8,540 - +0.3
8,716 - +0.3
9,006 + GFF1302 0.19 +0.6
9,006 + GFF1302 0.19 -0.8
9,007 - GFF1302 0.19 +0.1
9,087 - GFF1302 0.22 -0.0
9,087 - GFF1302 0.22 -0.1
9,222 - GFF1302 0.27 +0.7
9,269 + GFF1302 0.28 +0.7
9,270 - GFF1302 0.28 -0.3
9,338 - GFF1302 0.31 -0.7
9,338 - GFF1302 0.31 -1.1
9,369 - GFF1302 0.32 +0.6
9,369 - GFF1302 0.32 +0.3
9,467 + GFF1302 0.35 +1.4
9,515 + GFF1302 0.37 +0.1
9,516 - GFF1302 0.37 -0.6
9,558 - GFF1302 0.38 -0.1
9,622 + GFF1302 0.40 -0.2
9,622 + GFF1302 0.40 +0.6
9,721 + GFF1302 0.44 -0.2
9,944 + GFF1302 0.52 +0.2
10,112 - GFF1302 0.57 +1.1
10,181 - GFF1302 0.60 +0.5
10,188 - GFF1302 0.60 +3.2
10,337 + GFF1302 0.65 -0.5
10,401 + GFF1302 0.67 -0.3
10,405 + GFF1302 0.68 +0.3
10,482 - GFF1302 0.70 -0.5
10,572 - GFF1302 0.73 +1.5
10,629 - GFF1302 0.75 +0.4
10,658 + GFF1302 0.76 +3.2
10,917 + GFF1302 0.85 +0.1
11,010 - GFF1302 0.88 -0.1
11,010 - GFF1302 0.88 +0.2
11,066 + +0.4
11,066 + -0.8
11,066 + +0.1
11,067 - -0.1
11,244 - +0.7
11,359 + +0.5
11,392 + +0.5
11,420 - +0.8
11,422 + -0.4
11,423 - -1.5
11,456 + -0.9

Or see this region's nucleotide sequence