Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF130

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF128 and GFF129 overlap by 4 nucleotidesGFF129 and GFF130 are separated by 87 nucleotidesGFF130 and GFF131 are separated by 221 nucleotidesGFF131 and GFF132 are separated by 9 nucleotides GFF128 - AmpE protein, at 118,322 to 119,176 GFF128 GFF129 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 119,173 to 119,736 GFF129 GFF130 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 119,824 to 120,717 GFF130 GFF131 - Type IV pilin PilA, at 120,939 to 121,376 GFF131 GFF132 - Type IV fimbrial assembly, ATPase PilB, at 121,386 to 122,771 GFF132 Position (kb) 119 120 121Strain fitness (log2 ratio) -1 0 1 2 3at 118.905 kb on + strand, within GFF128at 118.906 kb on - strand, within GFF128at 118.911 kb on - strand, within GFF128at 118.934 kb on - strand, within GFF128at 118.983 kb on - strand, within GFF128at 118.994 kb on + strand, within GFF128at 119.084 kb on + strand, within GFF128at 119.156 kb on + strandat 119.164 kb on + strandat 119.307 kb on - strand, within GFF129at 119.349 kb on - strand, within GFF129at 119.477 kb on + strand, within GFF129at 119.477 kb on + strand, within GFF129at 119.477 kb on + strand, within GFF129at 119.478 kb on - strand, within GFF129at 119.632 kb on - strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.635 kb on - strand, within GFF129at 119.764 kb on + strandat 119.765 kb on - strandat 119.765 kb on - strandat 119.778 kb on + strandat 119.794 kb on + strandat 119.795 kb on - strandat 119.839 kb on + strandat 119.839 kb on + strandat 119.982 kb on + strand, within GFF130at 120.316 kb on + strand, within GFF130at 120.391 kb on - strand, within GFF130at 120.440 kb on + strand, within GFF130at 120.516 kb on + strand, within GFF130at 120.753 kb on - strandat 120.753 kb on - strandat 120.784 kb on - strandat 120.790 kb on + strandat 120.833 kb on - strandat 121.035 kb on + strand, within GFF131at 121.036 kb on - strand, within GFF131at 121.061 kb on + strand, within GFF131at 121.062 kb on - strand, within GFF131at 121.088 kb on + strand, within GFF131at 121.180 kb on - strand, within GFF131at 121.221 kb on - strand, within GFF131at 121.305 kb on - strand, within GFF131at 121.432 kb on + strandat 121.651 kb on + strand, within GFF132

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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118,905 + GFF128 0.68 +0.3
118,906 - GFF128 0.68 -0.2
118,911 - GFF128 0.69 +0.3
118,934 - GFF128 0.72 -0.3
118,983 - GFF128 0.77 -0.3
118,994 + GFF128 0.79 +0.1
119,084 + GFF128 0.89 +0.6
119,156 + +0.1
119,164 + -0.2
119,307 - GFF129 0.24 -0.2
119,349 - GFF129 0.31 +0.1
119,477 + GFF129 0.54 +0.6
119,477 + GFF129 0.54 +0.3
119,477 + GFF129 0.54 +1.0
119,478 - GFF129 0.54 +0.4
119,632 - GFF129 0.81 +2.2
119,634 + GFF129 0.82 +0.1
119,634 + GFF129 0.82 -0.4
119,634 + GFF129 0.82 +1.0
119,634 + GFF129 0.82 -0.4
119,635 - GFF129 0.82 +0.3
119,764 + +0.4
119,765 - -1.0
119,765 - +0.0
119,778 + +0.8
119,794 + -0.0
119,795 - -0.4
119,839 + -1.3
119,839 + +3.1
119,982 + GFF130 0.18 +1.9
120,316 + GFF130 0.55 +0.2
120,391 - GFF130 0.63 +0.0
120,440 + GFF130 0.69 +1.5
120,516 + GFF130 0.77 +0.7
120,753 - -0.4
120,753 - +0.0
120,784 - +1.8
120,790 + +2.7
120,833 - -0.7
121,035 + GFF131 0.22 -0.0
121,036 - GFF131 0.22 +0.2
121,061 + GFF131 0.28 -0.2
121,062 - GFF131 0.28 +0.5
121,088 + GFF131 0.34 -0.8
121,180 - GFF131 0.55 -0.1
121,221 - GFF131 0.64 -0.2
121,305 - GFF131 0.84 -0.1
121,432 + +0.6
121,651 + GFF132 0.19 -0.2

Or see this region's nucleotide sequence