Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF128 and GFF129 overlap by 4 nucleotides GFF129 and GFF130 are separated by 87 nucleotides GFF130 and GFF131 are separated by 221 nucleotides GFF131 and GFF132 are separated by 9 nucleotides
GFF128 - AmpE protein, at 118,322 to 119,176
GFF128
GFF129 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 119,173 to 119,736
GFF129
GFF130 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 119,824 to 120,717
GFF130
GFF131 - Type IV pilin PilA, at 120,939 to 121,376
GFF131
GFF132 - Type IV fimbrial assembly, ATPase PilB, at 121,386 to 122,771
GFF132
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-1
0
1
2
3 at 118.905 kb on + strand, within GFF128 at 118.906 kb on - strand, within GFF128 at 118.911 kb on - strand, within GFF128 at 118.934 kb on - strand, within GFF128 at 118.983 kb on - strand, within GFF128 at 118.994 kb on + strand, within GFF128 at 119.084 kb on + strand, within GFF128 at 119.156 kb on + strand at 119.164 kb on + strand at 119.307 kb on - strand, within GFF129 at 119.349 kb on - strand, within GFF129 at 119.477 kb on + strand, within GFF129 at 119.477 kb on + strand, within GFF129 at 119.477 kb on + strand, within GFF129 at 119.478 kb on - strand, within GFF129 at 119.632 kb on - strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.635 kb on - strand, within GFF129 at 119.764 kb on + strand at 119.765 kb on - strand at 119.765 kb on - strand at 119.778 kb on + strand at 119.794 kb on + strand at 119.795 kb on - strand at 119.839 kb on + strand at 119.839 kb on + strand at 119.982 kb on + strand, within GFF130 at 120.316 kb on + strand, within GFF130 at 120.391 kb on - strand, within GFF130 at 120.440 kb on + strand, within GFF130 at 120.516 kb on + strand, within GFF130 at 120.753 kb on - strand at 120.753 kb on - strand at 120.784 kb on - strand at 120.790 kb on + strand at 120.833 kb on - strand at 121.035 kb on + strand, within GFF131 at 121.036 kb on - strand, within GFF131 at 121.061 kb on + strand, within GFF131 at 121.062 kb on - strand, within GFF131 at 121.088 kb on + strand, within GFF131 at 121.180 kb on - strand, within GFF131 at 121.221 kb on - strand, within GFF131 at 121.305 kb on - strand, within GFF131 at 121.432 kb on + strand at 121.651 kb on + strand, within GFF132
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 118,905 + GFF128 0.68 +0.3 118,906 - GFF128 0.68 -0.2 118,911 - GFF128 0.69 +0.3 118,934 - GFF128 0.72 -0.3 118,983 - GFF128 0.77 -0.3 118,994 + GFF128 0.79 +0.1 119,084 + GFF128 0.89 +0.6 119,156 + +0.1 119,164 + -0.2 119,307 - GFF129 0.24 -0.2 119,349 - GFF129 0.31 +0.1 119,477 + GFF129 0.54 +0.6 119,477 + GFF129 0.54 +0.3 119,477 + GFF129 0.54 +1.0 119,478 - GFF129 0.54 +0.4 119,632 - GFF129 0.81 +2.2 119,634 + GFF129 0.82 +0.1 119,634 + GFF129 0.82 -0.4 119,634 + GFF129 0.82 +1.0 119,634 + GFF129 0.82 -0.4 119,635 - GFF129 0.82 +0.3 119,764 + +0.4 119,765 - -1.0 119,765 - +0.0 119,778 + +0.8 119,794 + -0.0 119,795 - -0.4 119,839 + -1.3 119,839 + +3.1 119,982 + GFF130 0.18 +1.9 120,316 + GFF130 0.55 +0.2 120,391 - GFF130 0.63 +0.0 120,440 + GFF130 0.69 +1.5 120,516 + GFF130 0.77 +0.7 120,753 - -0.4 120,753 - +0.0 120,784 - +1.8 120,790 + +2.7 120,833 - -0.7 121,035 + GFF131 0.22 -0.0 121,036 - GFF131 0.22 +0.2 121,061 + GFF131 0.28 -0.2 121,062 - GFF131 0.28 +0.5 121,088 + GFF131 0.34 -0.8 121,180 - GFF131 0.55 -0.1 121,221 - GFF131 0.64 -0.2 121,305 - GFF131 0.84 -0.1 121,432 + +0.6 121,651 + GFF132 0.19 -0.2
Or see this region's nucleotide sequence