Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1175

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1172 and GFF1173 overlap by 33 nucleotidesGFF1173 and GFF1174 are separated by 42 nucleotidesGFF1174 and GFF1175 overlap by 4 nucleotidesGFF1175 and GFF1176 are separated by 19 nucleotidesGFF1176 and GFF1177 are separated by 8 nucleotidesGFF1177 and GFF1178 overlap by 32 nucleotides GFF1172 - IncF plasmid conjugative transfer protein TrbC, at 19,816 to 20,454 GFF1172 GFF1173 - hypothetical protein, at 20,422 to 20,586 GFF1173 GFF1174 - FIG00642834: hypothetical protein, at 20,629 to 20,910 GFF1174 GFF1175 - IncF plasmid conjugative transfer protein TraN, at 20,907 to 22,733 GFF1175 GFF1176 - IncF plasmid conjugative transfer protein TrbE, at 22,753 to 22,959 GFF1176 GFF1177 - IncF plasmid conjugative transfer pilus assembly protein TraF, at 22,968 to 23,720 GFF1177 GFF1178 - hypothetical protein, at 23,689 to 23,820 GFF1178 Position (kb) 20 21 22 23Strain fitness (log2 ratio) -1 0 1 2 3 4at 20.162 kb on + strand, within GFF1172at 20.288 kb on - strand, within GFF1172at 20.288 kb on - strand, within GFF1172at 20.288 kb on - strand, within GFF1172at 20.321 kb on - strand, within GFF1172at 20.321 kb on - strand, within GFF1172at 20.335 kb on + strand, within GFF1172at 20.335 kb on + strand, within GFF1172at 20.443 kb on - strandat 20.752 kb on + strand, within GFF1174at 20.817 kb on + strand, within GFF1174at 20.850 kb on + strand, within GFF1174at 20.850 kb on + strand, within GFF1174at 20.850 kb on + strand, within GFF1174at 20.915 kb on + strandat 20.916 kb on - strandat 20.920 kb on + strandat 21.056 kb on + strandat 21.057 kb on - strandat 21.068 kb on + strandat 21.275 kb on + strand, within GFF1175at 21.275 kb on + strand, within GFF1175at 21.275 kb on + strand, within GFF1175at 21.276 kb on - strand, within GFF1175at 21.276 kb on - strand, within GFF1175at 21.340 kb on - strand, within GFF1175at 21.341 kb on + strand, within GFF1175at 21.341 kb on + strand, within GFF1175at 21.341 kb on + strand, within GFF1175at 21.341 kb on + strand, within GFF1175at 21.372 kb on + strand, within GFF1175at 21.373 kb on - strand, within GFF1175at 21.373 kb on - strand, within GFF1175at 21.432 kb on + strand, within GFF1175at 21.432 kb on + strand, within GFF1175at 21.450 kb on - strand, within GFF1175at 21.487 kb on + strand, within GFF1175at 21.546 kb on + strand, within GFF1175at 21.547 kb on - strand, within GFF1175at 21.573 kb on - strand, within GFF1175at 21.578 kb on + strand, within GFF1175at 21.578 kb on + strand, within GFF1175at 21.611 kb on + strand, within GFF1175at 21.611 kb on + strand, within GFF1175at 21.612 kb on - strand, within GFF1175at 21.612 kb on - strand, within GFF1175at 21.612 kb on - strand, within GFF1175at 21.612 kb on - strand, within GFF1175at 21.644 kb on + strand, within GFF1175at 21.645 kb on - strand, within GFF1175at 21.645 kb on - strand, within GFF1175at 21.686 kb on + strand, within GFF1175at 21.773 kb on - strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.984 kb on + strand, within GFF1175at 21.984 kb on + strand, within GFF1175at 21.985 kb on - strand, within GFF1175at 22.062 kb on + strand, within GFF1175at 22.062 kb on + strand, within GFF1175at 22.072 kb on - strand, within GFF1175at 22.220 kb on + strand, within GFF1175at 22.470 kb on + strand, within GFF1175at 22.602 kb on - strandat 22.731 kb on + strandat 22.732 kb on - strandat 22.751 kb on + strandat 22.751 kb on + strandat 22.752 kb on - strandat 22.786 kb on - strand, within GFF1176at 22.786 kb on - strand, within GFF1176at 22.826 kb on + strand, within GFF1176at 22.870 kb on - strand, within GFF1176at 22.870 kb on - strand, within GFF1176at 22.879 kb on + strand, within GFF1176at 22.879 kb on + strand, within GFF1176at 22.880 kb on - strand, within GFF1176at 22.880 kb on - strand, within GFF1176at 22.944 kb on + strandat 22.967 kb on + strandat 22.967 kb on + strandat 22.968 kb on - strandat 22.968 kb on - strandat 23.005 kb on + strandat 23.055 kb on + strand, within GFF1177at 23.056 kb on - strand, within GFF1177at 23.097 kb on - strand, within GFF1177at 23.130 kb on - strand, within GFF1177at 23.130 kb on - strand, within GFF1177at 23.217 kb on + strand, within GFF1177at 23.307 kb on + strand, within GFF1177at 23.307 kb on + strand, within GFF1177at 23.308 kb on - strand, within GFF1177at 23.308 kb on - strand, within GFF1177at 23.319 kb on + strand, within GFF1177at 23.329 kb on - strand, within GFF1177at 23.341 kb on + strand, within GFF1177at 23.342 kb on - strand, within GFF1177at 23.406 kb on + strand, within GFF1177at 23.407 kb on - strand, within GFF1177at 23.412 kb on - strand, within GFF1177at 23.425 kb on - strand, within GFF1177at 23.468 kb on + strand, within GFF1177at 23.487 kb on + strand, within GFF1177at 23.487 kb on + strand, within GFF1177at 23.487 kb on + strand, within GFF1177at 23.612 kb on - strand, within GFF1177at 23.655 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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20,162 + GFF1172 0.54 +0.9
20,288 - GFF1172 0.74 +0.7
20,288 - GFF1172 0.74 +0.2
20,288 - GFF1172 0.74 +0.8
20,321 - GFF1172 0.79 +0.1
20,321 - GFF1172 0.79 -1.5
20,335 + GFF1172 0.81 +1.9
20,335 + GFF1172 0.81 +0.1
20,443 - -1.0
20,752 + GFF1174 0.44 +0.1
20,817 + GFF1174 0.67 +0.2
20,850 + GFF1174 0.78 +0.3
20,850 + GFF1174 0.78 +0.0
20,850 + GFF1174 0.78 -1.6
20,915 + -0.2
20,916 - +0.1
20,920 + -0.1
21,056 + +2.8
21,057 - +3.0
21,068 + +0.4
21,275 + GFF1175 0.20 -1.1
21,275 + GFF1175 0.20 -0.1
21,275 + GFF1175 0.20 +1.3
21,276 - GFF1175 0.20 +1.0
21,276 - GFF1175 0.20 +0.9
21,340 - GFF1175 0.24 -0.5
21,341 + GFF1175 0.24 -0.6
21,341 + GFF1175 0.24 +0.4
21,341 + GFF1175 0.24 +1.2
21,341 + GFF1175 0.24 +1.9
21,372 + GFF1175 0.25 -0.7
21,373 - GFF1175 0.26 +0.4
21,373 - GFF1175 0.26 -0.7
21,432 + GFF1175 0.29 -0.1
21,432 + GFF1175 0.29 -0.3
21,450 - GFF1175 0.30 -0.1
21,487 + GFF1175 0.32 -0.6
21,546 + GFF1175 0.35 +0.1
21,547 - GFF1175 0.35 +1.0
21,573 - GFF1175 0.36 -0.2
21,578 + GFF1175 0.37 -0.3
21,578 + GFF1175 0.37 -0.9
21,611 + GFF1175 0.39 +0.1
21,611 + GFF1175 0.39 +3.9
21,612 - GFF1175 0.39 -0.7
21,612 - GFF1175 0.39 -0.1
21,612 - GFF1175 0.39 -0.6
21,612 - GFF1175 0.39 +0.1
21,644 + GFF1175 0.40 +0.1
21,645 - GFF1175 0.40 -1.2
21,645 - GFF1175 0.40 +0.7
21,686 + GFF1175 0.43 -0.1
21,773 - GFF1175 0.47 +0.3
21,945 + GFF1175 0.57 -0.4
21,945 + GFF1175 0.57 +0.4
21,945 + GFF1175 0.57 +0.0
21,945 + GFF1175 0.57 -0.3
21,945 + GFF1175 0.57 +3.0
21,946 - GFF1175 0.57 +0.2
21,946 - GFF1175 0.57 +0.3
21,946 - GFF1175 0.57 -1.6
21,946 - GFF1175 0.57 +0.0
21,984 + GFF1175 0.59 -1.5
21,984 + GFF1175 0.59 -0.2
21,985 - GFF1175 0.59 -0.9
22,062 + GFF1175 0.63 -0.1
22,062 + GFF1175 0.63 +0.6
22,072 - GFF1175 0.64 -1.5
22,220 + GFF1175 0.72 +0.4
22,470 + GFF1175 0.86 -0.7
22,602 - +0.5
22,731 + -0.2
22,732 - +0.7
22,751 + +0.6
22,751 + +1.5
22,752 - -0.0
22,786 - GFF1176 0.16 -0.7
22,786 - GFF1176 0.16 +0.9
22,826 + GFF1176 0.35 +0.2
22,870 - GFF1176 0.57 +0.1
22,870 - GFF1176 0.57 -0.0
22,879 + GFF1176 0.61 +1.7
22,879 + GFF1176 0.61 +1.3
22,880 - GFF1176 0.61 -0.2
22,880 - GFF1176 0.61 -0.3
22,944 + -0.3
22,967 + +1.0
22,967 + +1.7
22,968 - +1.1
22,968 - -1.5
23,005 + +2.0
23,055 + GFF1177 0.12 +1.0
23,056 - GFF1177 0.12 +0.3
23,097 - GFF1177 0.17 +0.3
23,130 - GFF1177 0.22 -0.1
23,130 - GFF1177 0.22 +0.3
23,217 + GFF1177 0.33 +1.0
23,307 + GFF1177 0.45 -0.4
23,307 + GFF1177 0.45 -0.2
23,308 - GFF1177 0.45 -0.1
23,308 - GFF1177 0.45 -0.8
23,319 + GFF1177 0.47 -0.2
23,329 - GFF1177 0.48 -0.7
23,341 + GFF1177 0.50 -0.6
23,342 - GFF1177 0.50 +1.3
23,406 + GFF1177 0.58 -1.5
23,407 - GFF1177 0.58 -0.2
23,412 - GFF1177 0.59 +0.4
23,425 - GFF1177 0.61 -0.7
23,468 + GFF1177 0.66 +0.3
23,487 + GFF1177 0.69 -0.6
23,487 + GFF1177 0.69 -0.9
23,487 + GFF1177 0.69 +0.4
23,612 - GFF1177 0.86 +0.6
23,655 - +0.5

Or see this region's nucleotide sequence