Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1172 and GFF1173 overlap by 33 nucleotides GFF1173 and GFF1174 are separated by 42 nucleotides GFF1174 and GFF1175 overlap by 4 nucleotides GFF1175 and GFF1176 are separated by 19 nucleotides GFF1176 and GFF1177 are separated by 8 nucleotides GFF1177 and GFF1178 overlap by 32 nucleotides
GFF1172 - IncF plasmid conjugative transfer protein TrbC, at 19,816 to 20,454
GFF1172
GFF1173 - hypothetical protein, at 20,422 to 20,586
GFF1173
GFF1174 - FIG00642834: hypothetical protein, at 20,629 to 20,910
GFF1174
GFF1175 - IncF plasmid conjugative transfer protein TraN, at 20,907 to 22,733
GFF1175
GFF1176 - IncF plasmid conjugative transfer protein TrbE, at 22,753 to 22,959
GFF1176
GFF1177 - IncF plasmid conjugative transfer pilus assembly protein TraF, at 22,968 to 23,720
GFF1177
GFF1178 - hypothetical protein, at 23,689 to 23,820
GFF1178
Position (kb)
20
21
22
23 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 20.162 kb on + strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.321 kb on - strand, within GFF1172 at 20.321 kb on - strand, within GFF1172 at 20.335 kb on + strand, within GFF1172 at 20.335 kb on + strand, within GFF1172 at 20.443 kb on - strand at 20.752 kb on + strand, within GFF1174 at 20.817 kb on + strand, within GFF1174 at 20.850 kb on + strand, within GFF1174 at 20.850 kb on + strand, within GFF1174 at 20.850 kb on + strand, within GFF1174 at 20.915 kb on + strand at 20.916 kb on - strand at 20.920 kb on + strand at 21.056 kb on + strand at 21.057 kb on - strand at 21.068 kb on + strand at 21.275 kb on + strand, within GFF1175 at 21.275 kb on + strand, within GFF1175 at 21.275 kb on + strand, within GFF1175 at 21.276 kb on - strand, within GFF1175 at 21.276 kb on - strand, within GFF1175 at 21.340 kb on - strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.372 kb on + strand, within GFF1175 at 21.373 kb on - strand, within GFF1175 at 21.373 kb on - strand, within GFF1175 at 21.432 kb on + strand, within GFF1175 at 21.432 kb on + strand, within GFF1175 at 21.450 kb on - strand, within GFF1175 at 21.487 kb on + strand, within GFF1175 at 21.546 kb on + strand, within GFF1175 at 21.547 kb on - strand, within GFF1175 at 21.573 kb on - strand, within GFF1175 at 21.578 kb on + strand, within GFF1175 at 21.578 kb on + strand, within GFF1175 at 21.611 kb on + strand, within GFF1175 at 21.611 kb on + strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.644 kb on + strand, within GFF1175 at 21.645 kb on - strand, within GFF1175 at 21.645 kb on - strand, within GFF1175 at 21.686 kb on + strand, within GFF1175 at 21.773 kb on - strand, within GFF1175 at 21.945 kb on + strand, within GFF1175 at 21.945 kb on + strand, within GFF1175 at 21.945 kb on + strand, within GFF1175 at 21.945 kb on + strand, within GFF1175 at 21.945 kb on + strand, within GFF1175 at 21.946 kb on - strand, within GFF1175 at 21.946 kb on - strand, within GFF1175 at 21.946 kb on - strand, within GFF1175 at 21.946 kb on - strand, within GFF1175 at 21.984 kb on + strand, within GFF1175 at 21.984 kb on + strand, within GFF1175 at 21.985 kb on - strand, within GFF1175 at 22.062 kb on + strand, within GFF1175 at 22.062 kb on + strand, within GFF1175 at 22.072 kb on - strand, within GFF1175 at 22.220 kb on + strand, within GFF1175 at 22.470 kb on + strand, within GFF1175 at 22.602 kb on - strand at 22.731 kb on + strand at 22.732 kb on - strand at 22.751 kb on + strand at 22.751 kb on + strand at 22.752 kb on - strand at 22.786 kb on - strand, within GFF1176 at 22.786 kb on - strand, within GFF1176 at 22.826 kb on + strand, within GFF1176 at 22.870 kb on - strand, within GFF1176 at 22.870 kb on - strand, within GFF1176 at 22.879 kb on + strand, within GFF1176 at 22.879 kb on + strand, within GFF1176 at 22.880 kb on - strand, within GFF1176 at 22.880 kb on - strand, within GFF1176 at 22.944 kb on + strand at 22.967 kb on + strand at 22.967 kb on + strand at 22.968 kb on - strand at 22.968 kb on - strand at 23.005 kb on + strand at 23.055 kb on + strand, within GFF1177 at 23.056 kb on - strand, within GFF1177 at 23.097 kb on - strand, within GFF1177 at 23.130 kb on - strand, within GFF1177 at 23.130 kb on - strand, within GFF1177 at 23.217 kb on + strand, within GFF1177 at 23.307 kb on + strand, within GFF1177 at 23.307 kb on + strand, within GFF1177 at 23.308 kb on - strand, within GFF1177 at 23.308 kb on - strand, within GFF1177 at 23.319 kb on + strand, within GFF1177 at 23.329 kb on - strand, within GFF1177 at 23.341 kb on + strand, within GFF1177 at 23.342 kb on - strand, within GFF1177 at 23.406 kb on + strand, within GFF1177 at 23.407 kb on - strand, within GFF1177 at 23.412 kb on - strand, within GFF1177 at 23.425 kb on - strand, within GFF1177 at 23.468 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.612 kb on - strand, within GFF1177 at 23.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 20,162 + GFF1172 0.54 +0.9 20,288 - GFF1172 0.74 +0.7 20,288 - GFF1172 0.74 +0.2 20,288 - GFF1172 0.74 +0.8 20,321 - GFF1172 0.79 +0.1 20,321 - GFF1172 0.79 -1.5 20,335 + GFF1172 0.81 +1.9 20,335 + GFF1172 0.81 +0.1 20,443 - -1.0 20,752 + GFF1174 0.44 +0.1 20,817 + GFF1174 0.67 +0.2 20,850 + GFF1174 0.78 +0.3 20,850 + GFF1174 0.78 +0.0 20,850 + GFF1174 0.78 -1.6 20,915 + -0.2 20,916 - +0.1 20,920 + -0.1 21,056 + +2.8 21,057 - +3.0 21,068 + +0.4 21,275 + GFF1175 0.20 -1.1 21,275 + GFF1175 0.20 -0.1 21,275 + GFF1175 0.20 +1.3 21,276 - GFF1175 0.20 +1.0 21,276 - GFF1175 0.20 +0.9 21,340 - GFF1175 0.24 -0.5 21,341 + GFF1175 0.24 -0.6 21,341 + GFF1175 0.24 +0.4 21,341 + GFF1175 0.24 +1.2 21,341 + GFF1175 0.24 +1.9 21,372 + GFF1175 0.25 -0.7 21,373 - GFF1175 0.26 +0.4 21,373 - GFF1175 0.26 -0.7 21,432 + GFF1175 0.29 -0.1 21,432 + GFF1175 0.29 -0.3 21,450 - GFF1175 0.30 -0.1 21,487 + GFF1175 0.32 -0.6 21,546 + GFF1175 0.35 +0.1 21,547 - GFF1175 0.35 +1.0 21,573 - GFF1175 0.36 -0.2 21,578 + GFF1175 0.37 -0.3 21,578 + GFF1175 0.37 -0.9 21,611 + GFF1175 0.39 +0.1 21,611 + GFF1175 0.39 +3.9 21,612 - GFF1175 0.39 -0.7 21,612 - GFF1175 0.39 -0.1 21,612 - GFF1175 0.39 -0.6 21,612 - GFF1175 0.39 +0.1 21,644 + GFF1175 0.40 +0.1 21,645 - GFF1175 0.40 -1.2 21,645 - GFF1175 0.40 +0.7 21,686 + GFF1175 0.43 -0.1 21,773 - GFF1175 0.47 +0.3 21,945 + GFF1175 0.57 -0.4 21,945 + GFF1175 0.57 +0.4 21,945 + GFF1175 0.57 +0.0 21,945 + GFF1175 0.57 -0.3 21,945 + GFF1175 0.57 +3.0 21,946 - GFF1175 0.57 +0.2 21,946 - GFF1175 0.57 +0.3 21,946 - GFF1175 0.57 -1.6 21,946 - GFF1175 0.57 +0.0 21,984 + GFF1175 0.59 -1.5 21,984 + GFF1175 0.59 -0.2 21,985 - GFF1175 0.59 -0.9 22,062 + GFF1175 0.63 -0.1 22,062 + GFF1175 0.63 +0.6 22,072 - GFF1175 0.64 -1.5 22,220 + GFF1175 0.72 +0.4 22,470 + GFF1175 0.86 -0.7 22,602 - +0.5 22,731 + -0.2 22,732 - +0.7 22,751 + +0.6 22,751 + +1.5 22,752 - -0.0 22,786 - GFF1176 0.16 -0.7 22,786 - GFF1176 0.16 +0.9 22,826 + GFF1176 0.35 +0.2 22,870 - GFF1176 0.57 +0.1 22,870 - GFF1176 0.57 -0.0 22,879 + GFF1176 0.61 +1.7 22,879 + GFF1176 0.61 +1.3 22,880 - GFF1176 0.61 -0.2 22,880 - GFF1176 0.61 -0.3 22,944 + -0.3 22,967 + +1.0 22,967 + +1.7 22,968 - +1.1 22,968 - -1.5 23,005 + +2.0 23,055 + GFF1177 0.12 +1.0 23,056 - GFF1177 0.12 +0.3 23,097 - GFF1177 0.17 +0.3 23,130 - GFF1177 0.22 -0.1 23,130 - GFF1177 0.22 +0.3 23,217 + GFF1177 0.33 +1.0 23,307 + GFF1177 0.45 -0.4 23,307 + GFF1177 0.45 -0.2 23,308 - GFF1177 0.45 -0.1 23,308 - GFF1177 0.45 -0.8 23,319 + GFF1177 0.47 -0.2 23,329 - GFF1177 0.48 -0.7 23,341 + GFF1177 0.50 -0.6 23,342 - GFF1177 0.50 +1.3 23,406 + GFF1177 0.58 -1.5 23,407 - GFF1177 0.58 -0.2 23,412 - GFF1177 0.59 +0.4 23,425 - GFF1177 0.61 -0.7 23,468 + GFF1177 0.66 +0.3 23,487 + GFF1177 0.69 -0.6 23,487 + GFF1177 0.69 -0.9 23,487 + GFF1177 0.69 +0.4 23,612 - GFF1177 0.86 +0.6 23,655 - +0.5
Or see this region's nucleotide sequence