Experiment: R2A with Spectinomycin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4108 and Atu4109 are separated by 70 nucleotides Atu4109 and Atu4110 are separated by 13 nucleotides Atu4110 and Atu4111 are separated by 54 nucleotides Atu4111 and Atu4112 are separated by 224 nucleotides
Atu4108: Atu4108 - ribonuclease D, at 1,228,027 to 1,228,653
Atu4108
Atu4109: Atu4109 - hypothetical protein, at 1,228,724 to 1,229,413
Atu4109
Atu4110: Atu4110 - serine dehydrogenase, at 1,229,427 to 1,230,176
Atu4110
Atu4111: Atu4111 - hypothetical protein, at 1,230,231 to 1,230,494
Atu4111
Atu4112: Atu4112 - proline dipeptidase, at 1,230,719 to 1,231,906
Atu4112
Position (kb)
1229
1230
1231 Strain fitness (log2 ratio)
-1
0
1 at 1228.464 kb on - strand, within Atu4108 at 1228.486 kb on - strand, within Atu4108 at 1228.647 kb on - strand at 1228.657 kb on - strand at 1228.896 kb on - strand, within Atu4109 at 1229.095 kb on - strand, within Atu4109 at 1229.112 kb on - strand, within Atu4109 at 1229.155 kb on + strand, within Atu4109 at 1229.188 kb on + strand, within Atu4109 at 1229.246 kb on + strand, within Atu4109 at 1229.333 kb on + strand, within Atu4109 at 1229.439 kb on + strand at 1229.472 kb on - strand at 1229.652 kb on - strand, within Atu4110 at 1229.652 kb on - strand, within Atu4110 at 1229.654 kb on + strand, within Atu4110 at 1229.680 kb on + strand, within Atu4110 at 1229.734 kb on + strand, within Atu4110 at 1229.768 kb on - strand, within Atu4110 at 1229.775 kb on - strand, within Atu4110 at 1229.778 kb on - strand, within Atu4110 at 1229.803 kb on - strand, within Atu4110 at 1229.849 kb on - strand, within Atu4110 at 1229.865 kb on + strand, within Atu4110 at 1229.865 kb on + strand, within Atu4110 at 1229.865 kb on + strand, within Atu4110 at 1229.873 kb on - strand, within Atu4110 at 1229.914 kb on + strand, within Atu4110 at 1229.995 kb on - strand, within Atu4110 at 1230.012 kb on + strand, within Atu4110 at 1230.115 kb on + strand at 1230.479 kb on + strand at 1230.590 kb on + strand at 1230.618 kb on + strand at 1230.631 kb on - strand at 1230.631 kb on - strand at 1230.631 kb on - strand at 1230.637 kb on - strand at 1230.737 kb on - strand at 1230.815 kb on - strand at 1230.882 kb on - strand, within Atu4112 at 1230.920 kb on + strand, within Atu4112 at 1230.920 kb on + strand, within Atu4112 at 1230.946 kb on + strand, within Atu4112 at 1230.965 kb on - strand, within Atu4112 at 1231.131 kb on + strand, within Atu4112 at 1231.131 kb on + strand, within Atu4112
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.002 mM remove 1,228,464 - Atu4108 0.70 -1.4 1,228,486 - Atu4108 0.73 -1.3 1,228,647 - +0.8 1,228,657 - +0.5 1,228,896 - Atu4109 0.25 +0.4 1,229,095 - Atu4109 0.54 -0.0 1,229,112 - Atu4109 0.56 +0.9 1,229,155 + Atu4109 0.62 +0.2 1,229,188 + Atu4109 0.67 +0.4 1,229,246 + Atu4109 0.76 -0.0 1,229,333 + Atu4109 0.88 -0.2 1,229,439 + +0.6 1,229,472 - +1.3 1,229,652 - Atu4110 0.30 +0.2 1,229,652 - Atu4110 0.30 -1.5 1,229,654 + Atu4110 0.30 -0.8 1,229,680 + Atu4110 0.34 -0.1 1,229,734 + Atu4110 0.41 +0.1 1,229,768 - Atu4110 0.45 -0.3 1,229,775 - Atu4110 0.46 +0.0 1,229,778 - Atu4110 0.47 -1.1 1,229,803 - Atu4110 0.50 +0.2 1,229,849 - Atu4110 0.56 +0.2 1,229,865 + Atu4110 0.58 -0.1 1,229,865 + Atu4110 0.58 +0.8 1,229,865 + Atu4110 0.58 +0.1 1,229,873 - Atu4110 0.59 +0.1 1,229,914 + Atu4110 0.65 -0.4 1,229,995 - Atu4110 0.76 -0.2 1,230,012 + Atu4110 0.78 -0.5 1,230,115 + -0.3 1,230,479 + +0.0 1,230,590 + +0.3 1,230,618 + +0.5 1,230,631 - +0.4 1,230,631 - +1.0 1,230,631 - -0.2 1,230,637 - +0.4 1,230,737 - -1.0 1,230,815 - -0.0 1,230,882 - Atu4112 0.14 +0.5 1,230,920 + Atu4112 0.17 +0.8 1,230,920 + Atu4112 0.17 -0.3 1,230,946 + Atu4112 0.19 -0.7 1,230,965 - Atu4112 0.21 +0.3 1,231,131 + Atu4112 0.35 +0.2 1,231,131 + Atu4112 0.35 +0.7
Or see this region's nucleotide sequence