Experiment: R2A with Spectinomycin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0345 and Atu0346 are separated by 96 nucleotides Atu0346 and Atu0347 are separated by 7 nucleotides
Atu0345: Atu0345 - DNA mismatch repair protein, MutS family, at 336,889 to 339,540
Atu0345
Atu0346: Atu0346 - uridylyltransferase/uridylyl-removing enzyme UTase, at 339,637 to 342,465
Atu0346
Atu0347: Atu0347 - virulence factor MviN, at 342,473 to 344,062
Atu0347
Position (kb)
339
340
341
342
343 Strain fitness (log2 ratio)
-2
-1
0
1 at 338.853 kb on + strand, within Atu0345 at 338.871 kb on + strand, within Atu0345 at 338.940 kb on - strand, within Atu0345 at 339.230 kb on + strand, within Atu0345 at 339.233 kb on - strand, within Atu0345 at 339.263 kb on + strand, within Atu0345 at 339.314 kb on + strand at 339.322 kb on + strand at 339.390 kb on + strand at 339.425 kb on + strand at 339.459 kb on - strand at 339.469 kb on - strand at 339.486 kb on + strand at 339.503 kb on + strand at 339.513 kb on - strand at 339.534 kb on + strand at 339.542 kb on - strand at 339.542 kb on - strand at 339.550 kb on + strand at 339.598 kb on + strand at 339.610 kb on + strand at 340.029 kb on + strand, within Atu0346 at 340.689 kb on + strand, within Atu0346 at 341.784 kb on + strand, within Atu0346 at 342.281 kb on + strand at 342.333 kb on + strand at 342.351 kb on + strand at 342.419 kb on + strand at 342.469 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.002 mM remove 338,853 + Atu0345 0.74 -0.9 338,871 + Atu0345 0.75 +1.3 338,940 - Atu0345 0.77 -0.4 339,230 + Atu0345 0.88 +0.3 339,233 - Atu0345 0.88 -0.4 339,263 + Atu0345 0.90 +0.0 339,314 + +0.4 339,322 + -0.8 339,390 + +1.1 339,425 + +1.1 339,459 - +1.4 339,469 - +0.2 339,486 + -0.3 339,503 + -0.0 339,513 - +0.5 339,534 + +0.7 339,542 - +0.5 339,542 - +1.0 339,550 + +0.1 339,598 + +0.9 339,610 + +0.3 340,029 + Atu0346 0.14 -0.9 340,689 + Atu0346 0.37 -0.3 341,784 + Atu0346 0.76 -1.8 342,281 + -0.3 342,333 + -0.1 342,351 + +0.0 342,419 + +0.0 342,469 + +0.2
Or see this region's nucleotide sequence