Experiment: R2A with Tetracycline 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5141 and Atu5142 are separated by 24 nucleotides Atu5142 and Atu5143 are separated by 278 nucleotides Atu5143 and Atu5144 are separated by 130 nucleotides Atu5144 and Atu5145 are separated by 69 nucleotides
Atu5141: Atu5141 - AraC family transcriptional regulator, at 147,842 to 148,606
Atu5141
Atu5142: Atu5142 - hypothetical protein, at 148,631 to 149,011
Atu5142
Atu5143: Atu5143 - transacetylase, at 149,290 to 150,114
Atu5143
Atu5144: Atu5144 - putative peptidase, at 150,245 to 151,000
Atu5144
Atu5145: Atu5145 - acetyltransferase, at 151,070 to 151,528
Atu5145
Position (kb)
149
150
151 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 148.294 kb on - strand, within Atu5141 at 148.297 kb on + strand, within Atu5141 at 148.338 kb on - strand, within Atu5141 at 148.387 kb on - strand, within Atu5141 at 148.407 kb on + strand, within Atu5141 at 148.419 kb on + strand, within Atu5141 at 148.463 kb on - strand, within Atu5141 at 148.481 kb on - strand, within Atu5141 at 148.486 kb on - strand, within Atu5141 at 148.523 kb on + strand, within Atu5141 at 148.562 kb on - strand at 148.583 kb on + strand at 148.583 kb on + strand at 148.596 kb on - strand at 148.596 kb on - strand at 148.633 kb on + strand at 148.641 kb on - strand at 148.659 kb on + strand at 148.782 kb on - strand, within Atu5142 at 148.791 kb on + strand, within Atu5142 at 148.940 kb on - strand, within Atu5142 at 148.946 kb on - strand, within Atu5142 at 149.044 kb on + strand at 149.044 kb on + strand at 149.094 kb on + strand at 149.094 kb on + strand at 149.094 kb on + strand at 149.094 kb on + strand at 149.102 kb on - strand at 149.102 kb on - strand at 149.107 kb on + strand at 149.130 kb on - strand at 149.132 kb on - strand at 149.191 kb on + strand at 149.191 kb on + strand at 149.199 kb on - strand at 149.257 kb on - strand at 149.257 kb on - strand at 149.290 kb on - strand at 149.326 kb on + strand at 149.458 kb on + strand, within Atu5143 at 149.476 kb on - strand, within Atu5143 at 149.501 kb on + strand, within Atu5143 at 149.531 kb on - strand, within Atu5143 at 149.549 kb on + strand, within Atu5143 at 149.557 kb on - strand, within Atu5143 at 149.593 kb on + strand, within Atu5143 at 149.593 kb on + strand, within Atu5143 at 149.601 kb on - strand, within Atu5143 at 149.638 kb on + strand, within Atu5143 at 149.646 kb on - strand, within Atu5143 at 149.710 kb on + strand, within Atu5143 at 149.724 kb on - strand, within Atu5143 at 149.734 kb on + strand, within Atu5143 at 149.740 kb on + strand, within Atu5143 at 149.756 kb on - strand, within Atu5143 at 149.790 kb on + strand, within Atu5143 at 149.840 kb on + strand, within Atu5143 at 149.840 kb on + strand, within Atu5143 at 149.841 kb on - strand, within Atu5143 at 149.876 kb on + strand, within Atu5143 at 149.943 kb on + strand, within Atu5143 at 149.946 kb on - strand, within Atu5143 at 150.003 kb on + strand, within Atu5143 at 150.018 kb on + strand, within Atu5143 at 150.078 kb on + strand at 150.086 kb on - strand at 150.086 kb on - strand at 150.149 kb on + strand at 150.157 kb on - strand at 150.246 kb on + strand at 150.261 kb on - strand at 150.267 kb on + strand at 150.267 kb on + strand at 150.267 kb on + strand at 150.275 kb on - strand at 150.285 kb on - strand at 150.469 kb on - strand, within Atu5144 at 150.527 kb on + strand, within Atu5144 at 150.550 kb on + strand, within Atu5144 at 150.569 kb on - strand, within Atu5144 at 150.663 kb on - strand, within Atu5144 at 150.672 kb on + strand, within Atu5144 at 150.704 kb on - strand, within Atu5144 at 150.743 kb on + strand, within Atu5144 at 150.801 kb on + strand, within Atu5144 at 150.876 kb on + strand, within Atu5144 at 150.884 kb on - strand, within Atu5144 at 150.937 kb on + strand at 150.942 kb on + strand at 150.942 kb on + strand at 150.942 kb on + strand at 150.942 kb on + strand at 150.942 kb on + strand at 150.942 kb on + strand at 150.945 kb on - strand at 150.950 kb on - strand at 150.950 kb on - strand at 150.950 kb on - strand at 151.018 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.002 mM remove 148,294 - Atu5141 0.59 +1.2 148,297 + Atu5141 0.59 -0.2 148,338 - Atu5141 0.65 -0.1 148,387 - Atu5141 0.71 +0.2 148,407 + Atu5141 0.74 +0.5 148,419 + Atu5141 0.75 -1.4 148,463 - Atu5141 0.81 +1.0 148,481 - Atu5141 0.84 +1.5 148,486 - Atu5141 0.84 +0.5 148,523 + Atu5141 0.89 -0.1 148,562 - -0.7 148,583 + -0.7 148,583 + +0.4 148,596 - -0.4 148,596 - +0.7 148,633 + +0.5 148,641 - -0.9 148,659 + -1.2 148,782 - Atu5142 0.40 -0.3 148,791 + Atu5142 0.42 -0.7 148,940 - Atu5142 0.81 +0.5 148,946 - Atu5142 0.83 -1.4 149,044 + -1.5 149,044 + +0.4 149,094 + -0.5 149,094 + -0.4 149,094 + +0.2 149,094 + +3.1 149,102 - -0.9 149,102 - -1.2 149,107 + +1.1 149,130 - -1.4 149,132 - -0.1 149,191 + +2.1 149,191 + +0.1 149,199 - +0.7 149,257 - +2.1 149,257 - +0.1 149,290 - +0.7 149,326 + +1.0 149,458 + Atu5143 0.20 -0.8 149,476 - Atu5143 0.23 +2.1 149,501 + Atu5143 0.26 -0.7 149,531 - Atu5143 0.29 -1.0 149,549 + Atu5143 0.31 -2.1 149,557 - Atu5143 0.32 +0.8 149,593 + Atu5143 0.37 +0.7 149,593 + Atu5143 0.37 +1.3 149,601 - Atu5143 0.38 +0.8 149,638 + Atu5143 0.42 -0.1 149,646 - Atu5143 0.43 +0.4 149,710 + Atu5143 0.51 -1.3 149,724 - Atu5143 0.53 +0.1 149,734 + Atu5143 0.54 +0.6 149,740 + Atu5143 0.55 +0.7 149,756 - Atu5143 0.56 +0.7 149,790 + Atu5143 0.61 -0.2 149,840 + Atu5143 0.67 +2.8 149,840 + Atu5143 0.67 +0.6 149,841 - Atu5143 0.67 +2.0 149,876 + Atu5143 0.71 +0.1 149,943 + Atu5143 0.79 +0.7 149,946 - Atu5143 0.80 +0.1 150,003 + Atu5143 0.86 +1.1 150,018 + Atu5143 0.88 -1.8 150,078 + -1.1 150,086 - +0.7 150,086 - -1.2 150,149 + +1.1 150,157 - +0.1 150,246 + +0.6 150,261 - -1.0 150,267 + -1.1 150,267 + -1.0 150,267 + +0.3 150,275 - -0.1 150,285 - -0.5 150,469 - Atu5144 0.30 +0.0 150,527 + Atu5144 0.37 +0.7 150,550 + Atu5144 0.40 +0.4 150,569 - Atu5144 0.43 +0.1 150,663 - Atu5144 0.55 +0.7 150,672 + Atu5144 0.56 +1.3 150,704 - Atu5144 0.61 +0.7 150,743 + Atu5144 0.66 -0.2 150,801 + Atu5144 0.74 -0.8 150,876 + Atu5144 0.83 +1.2 150,884 - Atu5144 0.85 +0.6 150,937 + -0.3 150,942 + +1.5 150,942 + +0.2 150,942 + +0.4 150,942 + +1.9 150,942 + +1.5 150,942 + -0.5 150,945 - +2.1 150,950 - -0.0 150,950 - +1.1 150,950 - -0.7 151,018 - +0.2
Or see this region's nucleotide sequence