Strain Fitness in Agrobacterium fabrum C58 around Atu1022

Experiment: R2A with Tetracycline 0.002 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu1021 and Atu1022 are separated by 157 nucleotidesAtu1022 and Atu1023 are separated by 22 nucleotidesAtu1023 and Atu1024 are separated by 64 nucleotides Atu1021: Atu1021 - outer membrane protein, at 1,013,942 to 1,015,039 Atu1021 Atu1022: Atu1022 - soluble lytic transglycosylase, at 1,015,197 to 1,017,245 Atu1022 Atu1023: Atu1023 - hypothetical protein, at 1,017,268 to 1,017,564 Atu1023 Atu1024: Atu1024 - dihydrodipicolinate synthase, at 1,017,629 to 1,018,513 Atu1024 Position (kb) 1015 1016 1017 1018Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1014.283 kb on + strand, within Atu1021at 1014.448 kb on + strand, within Atu1021at 1014.696 kb on + strand, within Atu1021at 1014.891 kb on + strand, within Atu1021at 1015.297 kb on - strandat 1015.359 kb on - strandat 1015.389 kb on - strandat 1015.533 kb on - strand, within Atu1022at 1015.681 kb on - strand, within Atu1022at 1015.721 kb on - strand, within Atu1022at 1015.721 kb on - strand, within Atu1022at 1015.748 kb on - strand, within Atu1022at 1015.820 kb on + strand, within Atu1022at 1015.861 kb on - strand, within Atu1022at 1015.896 kb on - strand, within Atu1022at 1015.963 kb on - strand, within Atu1022at 1015.963 kb on - strand, within Atu1022at 1016.137 kb on + strand, within Atu1022at 1016.157 kb on - strand, within Atu1022at 1016.195 kb on + strand, within Atu1022at 1016.209 kb on + strand, within Atu1022at 1016.217 kb on - strand, within Atu1022at 1016.433 kb on - strand, within Atu1022at 1016.478 kb on - strand, within Atu1022at 1016.478 kb on - strand, within Atu1022at 1016.484 kb on - strand, within Atu1022at 1016.519 kb on - strand, within Atu1022at 1016.577 kb on - strand, within Atu1022at 1016.615 kb on - strand, within Atu1022at 1016.778 kb on - strand, within Atu1022at 1016.895 kb on + strand, within Atu1022at 1017.004 kb on + strand, within Atu1022at 1017.004 kb on + strand, within Atu1022at 1017.004 kb on + strand, within Atu1022at 1017.004 kb on + strand, within Atu1022at 1017.012 kb on - strand, within Atu1022at 1017.058 kb on - strandat 1017.061 kb on + strandat 1017.069 kb on - strandat 1017.098 kb on - strandat 1017.166 kb on + strandat 1017.171 kb on + strandat 1017.171 kb on + strandat 1017.174 kb on - strandat 1017.179 kb on - strandat 1017.180 kb on + strandat 1017.187 kb on + strandat 1017.197 kb on - strandat 1017.385 kb on - strand, within Atu1023at 1017.558 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.002 mM
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1,014,283 + Atu1021 0.31 +2.6
1,014,448 + Atu1021 0.46 +2.7
1,014,696 + Atu1021 0.69 +3.0
1,014,891 + Atu1021 0.86 +2.5
1,015,297 - -1.2
1,015,359 - -0.1
1,015,389 - +1.3
1,015,533 - Atu1022 0.16 -1.1
1,015,681 - Atu1022 0.24 +1.6
1,015,721 - Atu1022 0.26 -0.7
1,015,721 - Atu1022 0.26 -1.0
1,015,748 - Atu1022 0.27 -0.3
1,015,820 + Atu1022 0.30 -1.8
1,015,861 - Atu1022 0.32 -0.5
1,015,896 - Atu1022 0.34 -0.6
1,015,963 - Atu1022 0.37 -0.0
1,015,963 - Atu1022 0.37 -1.0
1,016,137 + Atu1022 0.46 -0.8
1,016,157 - Atu1022 0.47 +0.6
1,016,195 + Atu1022 0.49 -0.4
1,016,209 + Atu1022 0.49 +1.5
1,016,217 - Atu1022 0.50 -0.2
1,016,433 - Atu1022 0.60 -0.2
1,016,478 - Atu1022 0.63 -0.4
1,016,478 - Atu1022 0.63 -0.8
1,016,484 - Atu1022 0.63 -0.8
1,016,519 - Atu1022 0.65 +0.3
1,016,577 - Atu1022 0.67 -0.1
1,016,615 - Atu1022 0.69 -1.3
1,016,778 - Atu1022 0.77 -1.1
1,016,895 + Atu1022 0.83 -0.2
1,017,004 + Atu1022 0.88 +0.2
1,017,004 + Atu1022 0.88 +0.6
1,017,004 + Atu1022 0.88 +0.8
1,017,004 + Atu1022 0.88 +0.5
1,017,012 - Atu1022 0.89 +0.6
1,017,058 - -1.8
1,017,061 + -2.0
1,017,069 - -0.4
1,017,098 - -1.2
1,017,166 + +1.3
1,017,171 + -0.9
1,017,171 + -0.5
1,017,174 - +1.1
1,017,179 - +0.6
1,017,180 + -0.7
1,017,187 + -2.0
1,017,197 - +0.5
1,017,385 - Atu1023 0.39 +0.0
1,017,558 + +1.0

Or see this region's nucleotide sequence