Experiment: Ponti_ML7_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA264_14985 and CA264_14990 are separated by 60 nucleotides CA264_14990 and CA264_14995 are separated by 325 nucleotides CA264_14995 and CA264_15000 are separated by 110 nucleotides CA264_15000 and CA264_15005 are separated by 143 nucleotides
CA264_14985: CA264_14985 - hypothetical protein, at 3,517,248 to 3,518,114
_14985
CA264_14990: CA264_14990 - integration host factor subunit beta, at 3,518,175 to 3,518,480
_14990
CA264_14995: CA264_14995 - A/G-specific adenine glycosylase, at 3,518,806 to 3,519,897
_14995
CA264_15000: CA264_15000 - single-stranded DNA-binding protein, at 3,520,008 to 3,520,487
_15000
CA264_15005: CA264_15005 - magnesium/cobalt efflux protein, at 3,520,631 to 3,521,884
_15005
Position (kb)
3518
3519
3520 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3517.860 kb on - strand, within CA264_14985 at 3518.040 kb on + strand at 3518.097 kb on + strand at 3518.592 kb on + strand at 3518.595 kb on + strand at 3518.802 kb on - strand at 3518.893 kb on - strand at 3518.906 kb on - strand at 3518.907 kb on + strand at 3518.907 kb on + strand at 3518.907 kb on + strand at 3518.907 kb on + strand at 3518.907 kb on + strand at 3518.907 kb on + strand at 3518.907 kb on + strand at 3518.908 kb on - strand at 3518.908 kb on - strand at 3518.908 kb on - strand at 3518.908 kb on - strand at 3518.965 kb on + strand, within CA264_14995 at 3519.058 kb on + strand, within CA264_14995 at 3519.133 kb on + strand, within CA264_14995 at 3519.153 kb on - strand, within CA264_14995 at 3519.170 kb on - strand, within CA264_14995 at 3519.380 kb on + strand, within CA264_14995 at 3519.381 kb on - strand, within CA264_14995 at 3519.515 kb on - strand, within CA264_14995 at 3519.547 kb on - strand, within CA264_14995 at 3519.561 kb on - strand, within CA264_14995 at 3519.756 kb on + strand, within CA264_14995 at 3519.757 kb on - strand, within CA264_14995 at 3519.804 kb on - strand at 3519.811 kb on + strand at 3519.862 kb on + strand at 3519.893 kb on + strand at 3519.893 kb on + strand at 3519.894 kb on - strand at 3519.947 kb on + strand at 3519.988 kb on + strand at 3519.989 kb on - strand at 3519.992 kb on - strand at 3519.992 kb on - strand at 3520.369 kb on - strand, within CA264_15000 at 3520.401 kb on + strand, within CA264_15000 at 3520.485 kb on + strand at 3520.486 kb on - strand at 3520.490 kb on + strand at 3520.490 kb on + strand at 3520.490 kb on + strand at 3520.491 kb on - strand at 3520.491 kb on - strand at 3520.491 kb on - strand at 3520.491 kb on - strand at 3520.522 kb on + strand at 3520.523 kb on - strand at 3520.548 kb on - strand at 3520.789 kb on + strand, within CA264_15005 at 3520.807 kb on - strand, within CA264_15005 at 3520.816 kb on + strand, within CA264_15005 at 3520.817 kb on - strand, within CA264_15005 at 3520.830 kb on - strand, within CA264_15005
Per-strain Table
Position Strand Gene LocusTag Fraction Ponti_ML7_MIT diversity barseq remove 3,517,860 - CA264_14985 0.71 +0.7 3,518,040 + +0.3 3,518,097 + -0.1 3,518,592 + -0.1 3,518,595 + +0.2 3,518,802 - +0.6 3,518,893 - +1.2 3,518,906 - +1.1 3,518,907 + +1.7 3,518,907 + +0.0 3,518,907 + -0.1 3,518,907 + -0.2 3,518,907 + +0.2 3,518,907 + +0.1 3,518,907 + +0.2 3,518,908 - +0.4 3,518,908 - -0.3 3,518,908 - +0.4 3,518,908 - +0.6 3,518,965 + CA264_14995 0.15 +0.6 3,519,058 + CA264_14995 0.23 -0.9 3,519,133 + CA264_14995 0.30 +1.6 3,519,153 - CA264_14995 0.32 +1.4 3,519,170 - CA264_14995 0.33 +0.9 3,519,380 + CA264_14995 0.53 -0.1 3,519,381 - CA264_14995 0.53 -0.0 3,519,515 - CA264_14995 0.65 +0.5 3,519,547 - CA264_14995 0.68 +0.0 3,519,561 - CA264_14995 0.69 -0.1 3,519,756 + CA264_14995 0.87 -1.0 3,519,757 - CA264_14995 0.87 +1.1 3,519,804 - -1.1 3,519,811 + +0.5 3,519,862 + -0.1 3,519,893 + -0.2 3,519,893 + -0.1 3,519,894 - +0.1 3,519,947 + +1.5 3,519,988 + +0.3 3,519,989 - -0.2 3,519,992 - +0.3 3,519,992 - +0.4 3,520,369 - CA264_15000 0.75 +1.5 3,520,401 + CA264_15000 0.82 +2.5 3,520,485 + +0.2 3,520,486 - -0.3 3,520,490 + -0.2 3,520,490 + -0.7 3,520,490 + +0.3 3,520,491 - -0.1 3,520,491 - -0.9 3,520,491 - -1.9 3,520,491 - +0.2 3,520,522 + -0.1 3,520,523 - -0.6 3,520,548 - +0.4 3,520,789 + CA264_15005 0.13 -0.7 3,520,807 - CA264_15005 0.14 +0.7 3,520,816 + CA264_15005 0.15 +0.2 3,520,817 - CA264_15005 0.15 -0.3 3,520,830 - CA264_15005 0.16 +0.3
Or see this region's nucleotide sequence