Experiment: R2A with Kanamycin sulfate 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4249 and Atu4250 are separated by 14 nucleotides Atu4250 and Atu4251 overlap by 11 nucleotides Atu4251 and Atu4252 are separated by 55 nucleotides
Atu4249: Atu4249 - sugars ABC transporter ATPase, at 1,373,286 to 1,374,329
Atu4249
Atu4250: Atu4250 - sugar ABC transporter permease, at 1,374,344 to 1,375,186
Atu4250
Atu4251: Atu4251 - sugar ABC transporter permease, at 1,375,176 to 1,376,102
Atu4251
Atu4252: Atu4252 - sugar ABC transporter substrate-binding protein, at 1,376,158 to 1,377,417
Atu4252
Position (kb)
1374
1375
1376 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1373.356 kb on + strand at 1373.477 kb on + strand, within Atu4249 at 1373.485 kb on - strand, within Atu4249 at 1373.530 kb on + strand, within Atu4249 at 1373.553 kb on + strand, within Atu4249 at 1373.647 kb on + strand, within Atu4249 at 1373.686 kb on - strand, within Atu4249 at 1373.733 kb on + strand, within Atu4249 at 1373.740 kb on + strand, within Atu4249 at 1373.829 kb on - strand, within Atu4249 at 1373.849 kb on + strand, within Atu4249 at 1373.912 kb on + strand, within Atu4249 at 1373.980 kb on + strand, within Atu4249 at 1373.980 kb on + strand, within Atu4249 at 1374.034 kb on - strand, within Atu4249 at 1374.059 kb on - strand, within Atu4249 at 1374.118 kb on - strand, within Atu4249 at 1374.213 kb on + strand, within Atu4249 at 1374.269 kb on + strand at 1374.280 kb on - strand at 1374.362 kb on + strand at 1374.368 kb on - strand at 1374.444 kb on + strand, within Atu4250 at 1374.460 kb on - strand, within Atu4250 at 1374.554 kb on - strand, within Atu4250 at 1374.611 kb on + strand, within Atu4250 at 1374.624 kb on + strand, within Atu4250 at 1374.624 kb on + strand, within Atu4250 at 1374.633 kb on + strand, within Atu4250 at 1374.641 kb on - strand, within Atu4250 at 1374.776 kb on + strand, within Atu4250 at 1374.806 kb on - strand, within Atu4250 at 1374.807 kb on + strand, within Atu4250 at 1374.986 kb on + strand, within Atu4250 at 1375.001 kb on - strand, within Atu4250 at 1375.011 kb on - strand, within Atu4250 at 1375.021 kb on + strand, within Atu4250 at 1375.138 kb on + strand at 1375.270 kb on - strand, within Atu4251 at 1375.277 kb on - strand, within Atu4251 at 1375.368 kb on - strand, within Atu4251 at 1375.368 kb on - strand, within Atu4251 at 1375.377 kb on + strand, within Atu4251 at 1375.378 kb on - strand, within Atu4251 at 1375.500 kb on - strand, within Atu4251 at 1375.500 kb on - strand, within Atu4251 at 1375.523 kb on - strand, within Atu4251 at 1375.523 kb on - strand, within Atu4251 at 1375.597 kb on + strand, within Atu4251 at 1375.605 kb on - strand, within Atu4251 at 1375.625 kb on - strand, within Atu4251 at 1375.676 kb on - strand, within Atu4251 at 1375.775 kb on + strand, within Atu4251 at 1375.789 kb on - strand, within Atu4251 at 1375.789 kb on - strand, within Atu4251 at 1375.814 kb on + strand, within Atu4251 at 1375.822 kb on - strand, within Atu4251 at 1375.822 kb on - strand, within Atu4251 at 1375.845 kb on + strand, within Atu4251 at 1375.880 kb on + strand, within Atu4251 at 1375.940 kb on - strand, within Atu4251 at 1376.038 kb on + strand at 1376.074 kb on - strand at 1376.097 kb on + strand at 1376.097 kb on + strand at 1376.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Kanamycin sulfate 0.02 mM remove 1,373,356 + -0.3 1,373,477 + Atu4249 0.18 +0.2 1,373,485 - Atu4249 0.19 +0.3 1,373,530 + Atu4249 0.23 +0.4 1,373,553 + Atu4249 0.26 +0.1 1,373,647 + Atu4249 0.35 -2.3 1,373,686 - Atu4249 0.38 +0.2 1,373,733 + Atu4249 0.43 -0.2 1,373,740 + Atu4249 0.43 -1.2 1,373,829 - Atu4249 0.52 +0.4 1,373,849 + Atu4249 0.54 +1.3 1,373,912 + Atu4249 0.60 +1.0 1,373,980 + Atu4249 0.66 +0.1 1,373,980 + Atu4249 0.66 -0.1 1,374,034 - Atu4249 0.72 -0.0 1,374,059 - Atu4249 0.74 +0.2 1,374,118 - Atu4249 0.80 +0.2 1,374,213 + Atu4249 0.89 -0.3 1,374,269 + -0.7 1,374,280 - -0.6 1,374,362 + -0.3 1,374,368 - +0.0 1,374,444 + Atu4250 0.12 -0.3 1,374,460 - Atu4250 0.14 -0.6 1,374,554 - Atu4250 0.25 -1.1 1,374,611 + Atu4250 0.32 +0.0 1,374,624 + Atu4250 0.33 +0.2 1,374,624 + Atu4250 0.33 +0.9 1,374,633 + Atu4250 0.34 -0.3 1,374,641 - Atu4250 0.35 -0.4 1,374,776 + Atu4250 0.51 +0.5 1,374,806 - Atu4250 0.55 +0.1 1,374,807 + Atu4250 0.55 +0.3 1,374,986 + Atu4250 0.76 +0.1 1,375,001 - Atu4250 0.78 -0.2 1,375,011 - Atu4250 0.79 +0.2 1,375,021 + Atu4250 0.80 +0.2 1,375,138 + +0.7 1,375,270 - Atu4251 0.10 -0.2 1,375,277 - Atu4251 0.11 -0.3 1,375,368 - Atu4251 0.21 +1.1 1,375,368 - Atu4251 0.21 -0.1 1,375,377 + Atu4251 0.22 +0.4 1,375,378 - Atu4251 0.22 +1.8 1,375,500 - Atu4251 0.35 -0.4 1,375,500 - Atu4251 0.35 +0.1 1,375,523 - Atu4251 0.37 +0.4 1,375,523 - Atu4251 0.37 -1.0 1,375,597 + Atu4251 0.45 +0.1 1,375,605 - Atu4251 0.46 +0.6 1,375,625 - Atu4251 0.48 +0.1 1,375,676 - Atu4251 0.54 -0.9 1,375,775 + Atu4251 0.65 +0.2 1,375,789 - Atu4251 0.66 -0.1 1,375,789 - Atu4251 0.66 +0.9 1,375,814 + Atu4251 0.69 +0.9 1,375,822 - Atu4251 0.70 -0.1 1,375,822 - Atu4251 0.70 -0.0 1,375,845 + Atu4251 0.72 +0.1 1,375,880 + Atu4251 0.76 +0.2 1,375,940 - Atu4251 0.82 +1.6 1,376,038 + -0.2 1,376,074 - -0.5 1,376,097 + -0.2 1,376,097 + -0.2 1,376,105 - +0.1
Or see this region's nucleotide sequence