Strain Fitness in Escherichia coli BL21 around ECD_01302

Experiment: Mixed culture, Halobacteriovorax-10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01300 and ECD_01301 are separated by 43 nucleotidesECD_01301 and ECD_01302 are separated by 118 nucleotidesECD_01302 and ECD_01303 overlap by 26 nucleotides ECD_01300: ECD_01300 - aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase, at 1,373,087 to 1,374,628 _01300 ECD_01301: ECD_01301 - lipid hydroperoxide peroxidase, at 1,374,672 to 1,375,178 _01301 ECD_01302: ECD_01302 - L-Ala-D/L-Glu epimerase, at 1,375,297 to 1,376,262 _01302 ECD_01303: ECD_01303 - murein peptide amidase A, at 1,376,237 to 1,376,965 _01303 Position (kb) 1375 1376 1377Strain fitness (log2 ratio) -1 0 1 2at 1374.303 kb on + strand, within ECD_01300at 1374.307 kb on + strand, within ECD_01300at 1374.352 kb on + strand, within ECD_01300at 1374.669 kb on - strandat 1374.827 kb on - strand, within ECD_01301at 1374.933 kb on - strand, within ECD_01301at 1375.334 kb on + strandat 1375.334 kb on + strandat 1375.334 kb on + strandat 1375.334 kb on + strandat 1375.413 kb on + strand, within ECD_01302at 1375.414 kb on - strand, within ECD_01302at 1375.983 kb on + strand, within ECD_01302at 1376.034 kb on + strand, within ECD_01302at 1376.130 kb on + strand, within ECD_01302at 1376.131 kb on - strand, within ECD_01302at 1376.589 kb on - strand, within ECD_01303at 1376.988 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Halobacteriovorax-10
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1,374,303 + ECD_01300 0.79 +0.1
1,374,307 + ECD_01300 0.79 +1.6
1,374,352 + ECD_01300 0.82 +0.6
1,374,669 - +0.5
1,374,827 - ECD_01301 0.31 -0.3
1,374,933 - ECD_01301 0.51 +1.1
1,375,334 + +0.4
1,375,334 + -0.1
1,375,334 + +0.2
1,375,334 + +0.2
1,375,413 + ECD_01302 0.12 -0.1
1,375,414 - ECD_01302 0.12 -1.0
1,375,983 + ECD_01302 0.71 +2.0
1,376,034 + ECD_01302 0.76 +0.3
1,376,130 + ECD_01302 0.86 +0.7
1,376,131 - ECD_01302 0.86 -0.5
1,376,589 - ECD_01303 0.48 -1.0
1,376,988 - -0.3

Or see this region's nucleotide sequence