Strain Fitness in Escherichia coli BL21 around ECD_00113

Experiment: Mixed culture, Halobacteriovorax-10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00112 and ECD_00113 are separated by 160 nucleotidesECD_00113 and ECD_00114 are separated by 14 nucleotides ECD_00112: ECD_00112 - pyruvate dehydrogenase complex repressor; autorepressor, at 124,897 to 125,661 _00112 ECD_00113: ECD_00113 - pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate-binding, at 125,822 to 128,485 _00113 ECD_00114: ECD_00114 - pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2, at 128,500 to 130,392 _00114 Position (kb) 125 126 127 128 129Strain fitness (log2 ratio) -1 0 1at 124.883 kb on + strandat 124.903 kb on + strandat 124.903 kb on + strandat 125.188 kb on + strand, within ECD_00112at 125.188 kb on + strand, within ECD_00112at 125.188 kb on + strand, within ECD_00112at 125.223 kb on + strand, within ECD_00112at 125.233 kb on + strand, within ECD_00112at 125.236 kb on + strand, within ECD_00112at 125.405 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.461 kb on + strand, within ECD_00112at 125.510 kb on + strand, within ECD_00112at 125.733 kb on + strandat 127.172 kb on + strand, within ECD_00113at 127.856 kb on + strand, within ECD_00113

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Halobacteriovorax-10
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124,883 + -0.5
124,903 + -0.6
124,903 + -0.4
125,188 + ECD_00112 0.38 -0.4
125,188 + ECD_00112 0.38 -0.5
125,188 + ECD_00112 0.38 -1.0
125,223 + ECD_00112 0.43 -0.7
125,233 + ECD_00112 0.44 -0.9
125,236 + ECD_00112 0.44 -0.6
125,405 + ECD_00112 0.66 -0.4
125,412 + ECD_00112 0.67 -0.4
125,412 + ECD_00112 0.67 -0.2
125,412 + ECD_00112 0.67 -0.8
125,412 + ECD_00112 0.67 -0.6
125,412 + ECD_00112 0.67 -0.2
125,461 + ECD_00112 0.74 -0.3
125,510 + ECD_00112 0.80 -0.3
125,733 + +0.1
127,172 + ECD_00113 0.51 -0.6
127,856 + ECD_00113 0.76 -1.2

Or see this region's nucleotide sequence