Experiment: R2A with rubidium chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3469 and Atu3470 overlap by 28 nucleotides Atu3470 and Atu3471 are separated by 194 nucleotides Atu3471 and Atu3472 are separated by 2 nucleotides
Atu3469: Atu3469 - phosphoglyceromutase, at 516,026 to 517,543
Atu3469
Atu3470: Atu3470 - hypothetical protein, at 517,516 to 517,710
Atu3470
Atu3471: Atu3471 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 517,905 to 519,182
Atu3471
Atu3472: Atu3472 - 2-oxoisovalerate dehydrogenase subunit beta, at 519,185 to 520,198
Atu3472
Position (kb)
517
518
519
520 Strain fitness (log2 ratio)
-2
-1
0
1 at 517.025 kb on - strand, within Atu3469 at 517.114 kb on + strand, within Atu3469 at 517.162 kb on + strand, within Atu3469 at 517.170 kb on - strand, within Atu3469 at 517.172 kb on - strand, within Atu3469 at 517.690 kb on - strand, within Atu3470 at 517.765 kb on - strand at 517.842 kb on + strand at 517.842 kb on + strand at 517.850 kb on - strand at 517.850 kb on - strand at 517.866 kb on + strand at 518.030 kb on + strand at 518.138 kb on - strand, within Atu3471 at 518.138 kb on - strand, within Atu3471 at 518.138 kb on - strand, within Atu3471 at 518.145 kb on - strand, within Atu3471 at 518.175 kb on - strand, within Atu3471 at 518.228 kb on + strand, within Atu3471 at 518.248 kb on - strand, within Atu3471 at 518.248 kb on - strand, within Atu3471 at 518.288 kb on - strand, within Atu3471 at 518.311 kb on + strand, within Atu3471 at 518.344 kb on + strand, within Atu3471 at 518.409 kb on + strand, within Atu3471 at 518.607 kb on - strand, within Atu3471 at 518.612 kb on - strand, within Atu3471 at 518.701 kb on + strand, within Atu3471 at 518.701 kb on + strand, within Atu3471 at 518.707 kb on + strand, within Atu3471 at 518.709 kb on - strand, within Atu3471 at 518.715 kb on - strand, within Atu3471 at 518.797 kb on + strand, within Atu3471 at 518.840 kb on - strand, within Atu3471 at 518.927 kb on - strand, within Atu3471 at 518.927 kb on - strand, within Atu3471 at 519.069 kb on + strand at 519.077 kb on - strand at 519.191 kb on - strand at 519.419 kb on - strand, within Atu3472 at 519.485 kb on - strand, within Atu3472 at 519.699 kb on + strand, within Atu3472 at 519.700 kb on + strand, within Atu3472 at 519.774 kb on - strand, within Atu3472 at 519.930 kb on - strand, within Atu3472 at 519.984 kb on - strand, within Atu3472 at 520.078 kb on - strand, within Atu3472 at 520.149 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with rubidium chloride 600 mM remove 517,025 - Atu3469 0.66 +0.1 517,114 + Atu3469 0.72 +0.8 517,162 + Atu3469 0.75 -0.0 517,170 - Atu3469 0.75 -1.3 517,172 - Atu3469 0.75 +0.7 517,690 - Atu3470 0.89 +1.0 517,765 - -0.5 517,842 + -0.7 517,842 + +0.4 517,850 - +0.8 517,850 - +0.5 517,866 + -0.0 518,030 + -0.1 518,138 - Atu3471 0.18 -0.7 518,138 - Atu3471 0.18 -0.4 518,138 - Atu3471 0.18 +0.1 518,145 - Atu3471 0.19 -0.2 518,175 - Atu3471 0.21 -0.9 518,228 + Atu3471 0.25 -0.9 518,248 - Atu3471 0.27 +0.4 518,248 - Atu3471 0.27 -0.2 518,288 - Atu3471 0.30 -1.0 518,311 + Atu3471 0.32 -0.2 518,344 + Atu3471 0.34 -0.9 518,409 + Atu3471 0.39 -0.0 518,607 - Atu3471 0.55 -1.8 518,612 - Atu3471 0.55 -0.3 518,701 + Atu3471 0.62 -1.2 518,701 + Atu3471 0.62 -0.4 518,707 + Atu3471 0.63 -0.3 518,709 - Atu3471 0.63 -1.3 518,715 - Atu3471 0.63 -0.5 518,797 + Atu3471 0.70 +0.2 518,840 - Atu3471 0.73 -0.6 518,927 - Atu3471 0.80 -1.0 518,927 - Atu3471 0.80 -1.5 519,069 + +0.1 519,077 - +1.1 519,191 - -0.5 519,419 - Atu3472 0.23 -0.9 519,485 - Atu3472 0.30 -1.4 519,699 + Atu3472 0.51 -0.7 519,700 + Atu3472 0.51 -0.5 519,774 - Atu3472 0.58 -1.7 519,930 - Atu3472 0.73 +0.5 519,984 - Atu3472 0.79 -0.0 520,078 - Atu3472 0.88 -0.5 520,149 + -0.4
Or see this region's nucleotide sequence