Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF991 and GFF992 are separated by 69 nucleotides GFF992 and GFF993 are separated by 71 nucleotides GFF993 and GFF994 overlap by 4 nucleotides GFF994 and GFF995 are separated by 70 nucleotides GFF995 and GFF996 are separated by 204 nucleotides GFF996 and GFF997 are separated by 74 nucleotides
GFF991 - hypothetical protein, at 176,692 to 176,835
GFF991
GFF992 - hypothetical protein, at 176,905 to 177,042
GFF992
GFF993 - Transposase STY4875, at 177,114 to 177,629
GFF993
GFF994 - hypothetical protein, at 177,626 to 177,739
GFF994
GFF995 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 177,810 to 179,180
GFF995
GFF996 - FIG074102: hypothetical protein, at 179,385 to 179,549
GFF996
GFF997 - Putative inner membrane protein, at 179,624 to 180,325
GFF997
Position (kb) 177
178
179
180 Strain fitness (log2 ratio) -1
0
1 at 176.831 kb on - strand at 176.878 kb on - strand at 176.909 kb on + strand at 176.934 kb on + strand, within GFF992 at 176.934 kb on + strand, within GFF992 at 176.934 kb on + strand, within GFF992 at 177.076 kb on + strand at 177.076 kb on + strand at 177.271 kb on + strand, within GFF993 at 177.272 kb on - strand, within GFF993 at 177.276 kb on - strand, within GFF993 at 177.314 kb on + strand, within GFF993 at 177.318 kb on + strand, within GFF993 at 177.318 kb on + strand, within GFF993 at 177.324 kb on - strand, within GFF993 at 177.395 kb on + strand, within GFF993 at 177.419 kb on - strand, within GFF993 at 177.684 kb on + strand, within GFF994 at 177.685 kb on - strand, within GFF994 at 177.689 kb on - strand, within GFF994 at 177.744 kb on - strand at 177.753 kb on - strand at 177.772 kb on - strand at 177.772 kb on - strand at 178.089 kb on + strand, within GFF995 at 178.092 kb on + strand, within GFF995 at 178.093 kb on - strand, within GFF995 at 178.364 kb on + strand, within GFF995 at 178.365 kb on - strand, within GFF995 at 178.478 kb on + strand, within GFF995 at 178.594 kb on + strand, within GFF995 at 178.594 kb on + strand, within GFF995 at 178.595 kb on - strand, within GFF995 at 178.595 kb on - strand, within GFF995 at 178.595 kb on - strand, within GFF995 at 178.598 kb on + strand, within GFF995 at 178.856 kb on + strand, within GFF995 at 178.859 kb on + strand, within GFF995 at 178.914 kb on + strand, within GFF995 at 178.914 kb on + strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 179.165 kb on - strand at 179.179 kb on - strand at 179.289 kb on + strand at 179.370 kb on + strand at 180.018 kb on - strand, within GFF997
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 176,831 - +0.1 176,878 - +0.2 176,909 + +0.0 176,934 + GFF992 0.21 -0.1 176,934 + GFF992 0.21 +0.5 176,934 + GFF992 0.21 -0.3 177,076 + -0.0 177,076 + -0.2 177,271 + GFF993 0.30 -0.3 177,272 - GFF993 0.31 +0.9 177,276 - GFF993 0.31 +0.5 177,314 + GFF993 0.39 +0.4 177,318 + GFF993 0.40 +1.0 177,318 + GFF993 0.40 -0.3 177,324 - GFF993 0.41 +0.1 177,395 + GFF993 0.54 +1.4 177,419 - GFF993 0.59 -0.4 177,684 + GFF994 0.51 +0.0 177,685 - GFF994 0.52 +1.0 177,689 - GFF994 0.55 -0.5 177,744 - +0.0 177,753 - -0.0 177,772 - +0.6 177,772 - -0.1 178,089 + GFF995 0.20 -0.3 178,092 + GFF995 0.21 -0.1 178,093 - GFF995 0.21 +0.7 178,364 + GFF995 0.40 +0.6 178,365 - GFF995 0.40 +0.1 178,478 + GFF995 0.49 -0.0 178,594 + GFF995 0.57 +1.2 178,594 + GFF995 0.57 -0.3 178,595 - GFF995 0.57 -0.0 178,595 - GFF995 0.57 +0.3 178,595 - GFF995 0.57 -0.0 178,598 + GFF995 0.57 -0.0 178,856 + GFF995 0.76 -0.2 178,859 + GFF995 0.77 +0.6 178,914 + GFF995 0.81 +0.2 178,914 + GFF995 0.81 +0.8 178,915 - GFF995 0.81 +0.6 178,915 - GFF995 0.81 +0.3 178,915 - GFF995 0.81 -0.1 178,915 - GFF995 0.81 +0.1 179,165 - +0.4 179,179 - +0.5 179,289 + +0.5 179,370 + +0.0 180,018 - GFF997 0.56 +0.2
Or see this region's nucleotide sequence