Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4635 and GFF4636 overlap by 7 nucleotides GFF4636 and GFF4637 overlap by 1 nucleotides GFF4637 and GFF4638 are separated by 13 nucleotides
GFF4635 - Succinate dehydrogenase cytochrome b-556 subunit, at 428,638 to 428,925
GFF4635
GFF4636 - Succinate dehydrogenase hydrophobic membrane anchor protein, at 428,919 to 429,266
GFF4636
GFF4637 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 429,266 to 431,032
GFF4637
GFF4638 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 431,046 to 431,765
GFF4638
Position (kb) 429
430
431
432 Strain fitness (log2 ratio) -2
-1
0
1 at 428.325 kb on - strand at 428.354 kb on + strand at 428.389 kb on - strand at 428.663 kb on + strand at 428.666 kb on + strand at 428.666 kb on + strand at 428.667 kb on - strand, within GFF4635 at 428.782 kb on + strand, within GFF4635 at 428.828 kb on - strand, within GFF4635 at 428.956 kb on + strand, within GFF4636 at 428.956 kb on + strand, within GFF4636 at 429.003 kb on + strand, within GFF4636 at 429.003 kb on + strand, within GFF4636 at 429.012 kb on + strand, within GFF4636 at 429.229 kb on - strand, within GFF4636 at 429.229 kb on - strand, within GFF4636 at 429.237 kb on + strand at 429.237 kb on + strand at 429.473 kb on + strand, within GFF4637 at 429.474 kb on - strand, within GFF4637 at 429.474 kb on - strand, within GFF4637 at 429.524 kb on + strand, within GFF4637 at 429.524 kb on + strand, within GFF4637 at 429.525 kb on - strand, within GFF4637 at 429.526 kb on + strand, within GFF4637 at 429.527 kb on - strand, within GFF4637 at 429.527 kb on - strand, within GFF4637 at 429.527 kb on - strand, within GFF4637 at 429.604 kb on + strand, within GFF4637 at 429.604 kb on + strand, within GFF4637 at 429.608 kb on + strand, within GFF4637 at 429.702 kb on + strand, within GFF4637 at 429.714 kb on - strand, within GFF4637 at 429.714 kb on - strand, within GFF4637 at 429.758 kb on + strand, within GFF4637 at 429.890 kb on + strand, within GFF4637 at 430.253 kb on - strand, within GFF4637 at 430.329 kb on - strand, within GFF4637 at 430.664 kb on - strand, within GFF4637 at 430.664 kb on - strand, within GFF4637 at 430.672 kb on + strand, within GFF4637 at 430.683 kb on + strand, within GFF4637 at 430.783 kb on + strand, within GFF4637 at 430.783 kb on + strand, within GFF4637 at 430.784 kb on - strand, within GFF4637 at 430.786 kb on + strand, within GFF4637 at 430.933 kb on + strand at 431.030 kb on + strand at 431.106 kb on - strand at 431.274 kb on - strand, within GFF4638 at 431.277 kb on - strand, within GFF4638 at 431.495 kb on + strand, within GFF4638 at 431.495 kb on + strand, within GFF4638 at 431.495 kb on + strand, within GFF4638 at 431.735 kb on + strand at 431.736 kb on - strand at 431.736 kb on - strand at 431.763 kb on + strand at 431.767 kb on + strand at 431.795 kb on + strand at 431.795 kb on + strand at 431.900 kb on - strand at 431.903 kb on - strand at 431.957 kb on + strand at 431.957 kb on + strand at 431.957 kb on + strand at 431.958 kb on - strand at 431.961 kb on + strand at 431.961 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 428,325 - -1.4 428,354 + -0.5 428,389 - -1.6 428,663 + -1.2 428,666 + -0.2 428,666 + -0.3 428,667 - GFF4635 0.10 -2.3 428,782 + GFF4635 0.50 -0.7 428,828 - GFF4635 0.66 -0.4 428,956 + GFF4636 0.11 -0.1 428,956 + GFF4636 0.11 -0.9 429,003 + GFF4636 0.24 -1.5 429,003 + GFF4636 0.24 -0.7 429,012 + GFF4636 0.27 -1.3 429,229 - GFF4636 0.89 -1.7 429,229 - GFF4636 0.89 -1.0 429,237 + +0.0 429,237 + -0.3 429,473 + GFF4637 0.12 -2.4 429,474 - GFF4637 0.12 -0.8 429,474 - GFF4637 0.12 -1.0 429,524 + GFF4637 0.15 -1.1 429,524 + GFF4637 0.15 -1.0 429,525 - GFF4637 0.15 -1.7 429,526 + GFF4637 0.15 -0.6 429,527 - GFF4637 0.15 -0.5 429,527 - GFF4637 0.15 -1.9 429,527 - GFF4637 0.15 -2.1 429,604 + GFF4637 0.19 -1.1 429,604 + GFF4637 0.19 -0.9 429,608 + GFF4637 0.19 -0.9 429,702 + GFF4637 0.25 -1.6 429,714 - GFF4637 0.25 -1.6 429,714 - GFF4637 0.25 -2.1 429,758 + GFF4637 0.28 -0.9 429,890 + GFF4637 0.35 +0.2 430,253 - GFF4637 0.56 -1.0 430,329 - GFF4637 0.60 -0.9 430,664 - GFF4637 0.79 -0.7 430,664 - GFF4637 0.79 -0.7 430,672 + GFF4637 0.80 -1.1 430,683 + GFF4637 0.80 +0.1 430,783 + GFF4637 0.86 -0.9 430,783 + GFF4637 0.86 -0.6 430,784 - GFF4637 0.86 -0.8 430,786 + GFF4637 0.86 +0.2 430,933 + -2.2 431,030 + -0.9 431,106 - -1.9 431,274 - GFF4638 0.32 -1.1 431,277 - GFF4638 0.32 -0.8 431,495 + GFF4638 0.62 -1.0 431,495 + GFF4638 0.62 -0.3 431,495 + GFF4638 0.62 -2.0 431,735 + -1.1 431,736 - -1.5 431,736 - -1.7 431,763 + +0.0 431,767 + -0.3 431,795 + -0.5 431,795 + -0.3 431,900 - -2.6 431,903 - +0.6 431,957 + -0.1 431,957 + +0.2 431,957 + -0.1 431,958 - -1.0 431,961 + -0.3 431,961 + +0.8
Or see this region's nucleotide sequence