Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4635 and GFF4636 overlap by 7 nucleotides GFF4636 and GFF4637 overlap by 1 nucleotides
GFF4635 - Succinate dehydrogenase cytochrome b-556 subunit, at 428,638 to 428,925
GFF4635
GFF4636 - Succinate dehydrogenase hydrophobic membrane anchor protein, at 428,919 to 429,266
GFF4636
GFF4637 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 429,266 to 431,032
GFF4637
Position (kb) 428
429
430 Strain fitness (log2 ratio) -2
-1
0
1 at 428.016 kb on + strand at 428.033 kb on + strand at 428.043 kb on - strand at 428.051 kb on + strand at 428.084 kb on + strand at 428.085 kb on - strand at 428.085 kb on - strand at 428.140 kb on - strand at 428.265 kb on + strand at 428.265 kb on + strand at 428.325 kb on - strand at 428.354 kb on + strand at 428.389 kb on - strand at 428.663 kb on + strand at 428.666 kb on + strand at 428.666 kb on + strand at 428.667 kb on - strand, within GFF4635 at 428.782 kb on + strand, within GFF4635 at 428.828 kb on - strand, within GFF4635 at 428.956 kb on + strand, within GFF4636 at 428.956 kb on + strand, within GFF4636 at 429.003 kb on + strand, within GFF4636 at 429.003 kb on + strand, within GFF4636 at 429.012 kb on + strand, within GFF4636 at 429.229 kb on - strand, within GFF4636 at 429.229 kb on - strand, within GFF4636 at 429.237 kb on + strand at 429.237 kb on + strand at 429.473 kb on + strand, within GFF4637 at 429.474 kb on - strand, within GFF4637 at 429.474 kb on - strand, within GFF4637 at 429.524 kb on + strand, within GFF4637 at 429.524 kb on + strand, within GFF4637 at 429.525 kb on - strand, within GFF4637 at 429.526 kb on + strand, within GFF4637 at 429.527 kb on - strand, within GFF4637 at 429.527 kb on - strand, within GFF4637 at 429.527 kb on - strand, within GFF4637 at 429.604 kb on + strand, within GFF4637 at 429.604 kb on + strand, within GFF4637 at 429.608 kb on + strand, within GFF4637 at 429.702 kb on + strand, within GFF4637 at 429.714 kb on - strand, within GFF4637 at 429.714 kb on - strand, within GFF4637 at 429.758 kb on + strand, within GFF4637 at 429.890 kb on + strand, within GFF4637 at 430.253 kb on - strand, within GFF4637
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 428,016 + -1.2 428,033 + -1.5 428,043 - +1.5 428,051 + -0.8 428,084 + -0.5 428,085 - -0.4 428,085 - -0.0 428,140 - -0.2 428,265 + -0.3 428,265 + -0.8 428,325 - -1.4 428,354 + -0.5 428,389 - -1.6 428,663 + -1.2 428,666 + -0.2 428,666 + -0.3 428,667 - GFF4635 0.10 -2.3 428,782 + GFF4635 0.50 -0.7 428,828 - GFF4635 0.66 -0.4 428,956 + GFF4636 0.11 -0.1 428,956 + GFF4636 0.11 -0.9 429,003 + GFF4636 0.24 -1.5 429,003 + GFF4636 0.24 -0.7 429,012 + GFF4636 0.27 -1.3 429,229 - GFF4636 0.89 -1.7 429,229 - GFF4636 0.89 -1.0 429,237 + +0.0 429,237 + -0.3 429,473 + GFF4637 0.12 -2.4 429,474 - GFF4637 0.12 -0.8 429,474 - GFF4637 0.12 -1.0 429,524 + GFF4637 0.15 -1.1 429,524 + GFF4637 0.15 -1.0 429,525 - GFF4637 0.15 -1.7 429,526 + GFF4637 0.15 -0.6 429,527 - GFF4637 0.15 -0.5 429,527 - GFF4637 0.15 -1.9 429,527 - GFF4637 0.15 -2.1 429,604 + GFF4637 0.19 -1.1 429,604 + GFF4637 0.19 -0.9 429,608 + GFF4637 0.19 -0.9 429,702 + GFF4637 0.25 -1.6 429,714 - GFF4637 0.25 -1.6 429,714 - GFF4637 0.25 -2.1 429,758 + GFF4637 0.28 -0.9 429,890 + GFF4637 0.35 +0.2 430,253 - GFF4637 0.56 -1.0
Or see this region's nucleotide sequence