Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4451 and GFF4452 are separated by 14 nucleotides GFF4452 and GFF4453 are separated by 9 nucleotides GFF4453 and GFF4454 are separated by 45 nucleotides
GFF4451 - Outer membrane usher protein SfmD, at 236,262 to 238,874
GFF4451
GFF4452 - Fimbriae-like adhesin SfmH, at 238,889 to 239,896
GFF4452
GFF4453 - Fimbriae-like periplasmic protein SfmF, at 239,906 to 240,424
GFF4453
GFF4454 - Transcriptional regulator of fimbriae expression FimZ (LuxR/UhpA family), at 240,470 to 241,102
GFF4454
Position (kb)
238
239
240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 237.893 kb on + strand, within GFF4451 at 237.908 kb on + strand, within GFF4451 at 237.915 kb on + strand, within GFF4451 at 237.949 kb on + strand, within GFF4451 at 238.022 kb on + strand, within GFF4451 at 238.023 kb on - strand, within GFF4451 at 238.073 kb on + strand, within GFF4451 at 238.085 kb on + strand, within GFF4451 at 238.086 kb on - strand, within GFF4451 at 238.086 kb on - strand, within GFF4451 at 238.089 kb on + strand, within GFF4451 at 238.131 kb on - strand, within GFF4451 at 238.173 kb on - strand, within GFF4451 at 238.203 kb on - strand, within GFF4451 at 238.208 kb on + strand, within GFF4451 at 238.218 kb on + strand, within GFF4451 at 238.220 kb on + strand, within GFF4451 at 238.221 kb on - strand, within GFF4451 at 238.221 kb on - strand, within GFF4451 at 238.255 kb on - strand, within GFF4451 at 238.282 kb on - strand, within GFF4451 at 238.282 kb on - strand, within GFF4451 at 238.305 kb on - strand, within GFF4451 at 238.305 kb on - strand, within GFF4451 at 238.346 kb on + strand, within GFF4451 at 238.481 kb on - strand, within GFF4451 at 238.490 kb on + strand, within GFF4451 at 238.508 kb on + strand, within GFF4451 at 238.509 kb on - strand, within GFF4451 at 238.606 kb on + strand, within GFF4451 at 238.698 kb on - strand at 238.800 kb on + strand at 239.133 kb on - strand, within GFF4452 at 239.133 kb on - strand, within GFF4452 at 239.136 kb on + strand, within GFF4452 at 239.177 kb on + strand, within GFF4452 at 239.195 kb on + strand, within GFF4452 at 239.239 kb on - strand, within GFF4452 at 239.258 kb on + strand, within GFF4452 at 239.281 kb on + strand, within GFF4452 at 239.362 kb on + strand, within GFF4452 at 239.399 kb on + strand, within GFF4452 at 239.400 kb on - strand, within GFF4452 at 239.440 kb on - strand, within GFF4452 at 239.441 kb on + strand, within GFF4452 at 239.450 kb on - strand, within GFF4452 at 239.541 kb on + strand, within GFF4452 at 239.605 kb on + strand, within GFF4452 at 239.614 kb on + strand, within GFF4452 at 239.720 kb on - strand, within GFF4452 at 239.722 kb on + strand, within GFF4452 at 239.806 kb on + strand at 240.167 kb on - strand, within GFF4453 at 240.167 kb on - strand, within GFF4453 at 240.351 kb on - strand, within GFF4453 at 240.353 kb on - strand, within GFF4453 at 240.422 kb on + strand at 240.423 kb on - strand at 240.471 kb on + strand at 240.539 kb on + strand, within GFF4454
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 237,893 + GFF4451 0.62 -0.4 237,908 + GFF4451 0.63 -0.1 237,915 + GFF4451 0.63 -0.3 237,949 + GFF4451 0.65 -0.5 238,022 + GFF4451 0.67 +0.8 238,023 - GFF4451 0.67 -0.4 238,073 + GFF4451 0.69 +0.1 238,085 + GFF4451 0.70 -0.7 238,086 - GFF4451 0.70 -0.2 238,086 - GFF4451 0.70 +0.1 238,089 + GFF4451 0.70 -0.0 238,131 - GFF4451 0.72 -0.0 238,173 - GFF4451 0.73 +0.8 238,203 - GFF4451 0.74 +0.6 238,208 + GFF4451 0.74 +0.1 238,218 + GFF4451 0.75 +0.2 238,220 + GFF4451 0.75 +1.1 238,221 - GFF4451 0.75 +0.8 238,221 - GFF4451 0.75 +0.9 238,255 - GFF4451 0.76 -0.2 238,282 - GFF4451 0.77 -1.2 238,282 - GFF4451 0.77 -0.7 238,305 - GFF4451 0.78 -0.9 238,305 - GFF4451 0.78 -0.3 238,346 + GFF4451 0.80 -0.5 238,481 - GFF4451 0.85 -0.4 238,490 + GFF4451 0.85 -0.5 238,508 + GFF4451 0.86 -0.4 238,509 - GFF4451 0.86 +0.2 238,606 + GFF4451 0.90 +0.0 238,698 - -0.0 238,800 + -0.0 239,133 - GFF4452 0.24 -0.9 239,133 - GFF4452 0.24 +0.2 239,136 + GFF4452 0.25 -0.4 239,177 + GFF4452 0.29 -1.1 239,195 + GFF4452 0.30 -0.1 239,239 - GFF4452 0.35 -1.2 239,258 + GFF4452 0.37 -1.0 239,281 + GFF4452 0.39 -0.8 239,362 + GFF4452 0.47 +0.1 239,399 + GFF4452 0.51 -1.0 239,400 - GFF4452 0.51 -0.8 239,440 - GFF4452 0.55 -0.8 239,441 + GFF4452 0.55 -0.0 239,450 - GFF4452 0.56 +0.3 239,541 + GFF4452 0.65 -3.4 239,605 + GFF4452 0.71 +1.1 239,614 + GFF4452 0.72 +0.2 239,720 - GFF4452 0.82 +0.5 239,722 + GFF4452 0.83 +0.6 239,806 + -0.1 240,167 - GFF4453 0.50 -0.6 240,167 - GFF4453 0.50 +0.6 240,351 - GFF4453 0.86 -0.4 240,353 - GFF4453 0.86 -0.5 240,422 + +0.6 240,423 - -0.3 240,471 + +0.2 240,539 + GFF4454 0.11 -0.2
Or see this region's nucleotide sequence