Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4198 and GFF4199 overlap by 1 nucleotides GFF4199 and GFF4200 overlap by 8 nucleotides GFF4200 and GFF4201 overlap by 20 nucleotides GFF4201 and GFF4202 are separated by 1 nucleotides
GFF4198 - Thiamin biosynthesis protein ThiC, at 12,199 to 14,094
GFF4198
GFF4199 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 14,094 to 14,729
GFF4199
GFF4200 - Sulfur carrier protein adenylyltransferase ThiF, at 14,722 to 15,480
GFF4200
GFF4201 - Sulfur carrier protein ThiS, at 15,461 to 15,661
GFF4201
GFF4202 - Thiazole biosynthesis protein ThiG, at 15,663 to 16,433
GFF4202
Position (kb) 14
15 Strain fitness (log2 ratio) -1
0
1 at 13.129 kb on - strand, within GFF4198 at 13.155 kb on - strand, within GFF4198 at 13.216 kb on + strand, within GFF4198 at 13.440 kb on + strand, within GFF4198 at 13.440 kb on + strand, within GFF4198 at 13.521 kb on + strand, within GFF4198 at 13.521 kb on + strand, within GFF4198 at 13.576 kb on + strand, within GFF4198 at 13.682 kb on - strand, within GFF4198 at 14.014 kb on - strand at 14.097 kb on + strand at 14.097 kb on + strand at 14.098 kb on - strand at 14.098 kb on - strand at 14.321 kb on + strand, within GFF4199 at 14.321 kb on + strand, within GFF4199 at 14.322 kb on - strand, within GFF4199 at 14.430 kb on - strand, within GFF4199 at 14.466 kb on + strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.549 kb on + strand, within GFF4199 at 14.660 kb on + strand, within GFF4199 at 14.813 kb on + strand, within GFF4200 at 14.813 kb on + strand, within GFF4200 at 14.814 kb on - strand, within GFF4200 at 15.194 kb on + strand, within GFF4200 at 15.194 kb on + strand, within GFF4200 at 15.195 kb on - strand, within GFF4200 at 15.662 kb on + strand at 15.662 kb on + strand at 15.667 kb on + strand at 15.667 kb on + strand at 15.667 kb on + strand at 15.668 kb on - strand at 15.671 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 13,129 - GFF4198 0.49 -0.0 13,155 - GFF4198 0.50 -0.7 13,216 + GFF4198 0.54 +0.2 13,440 + GFF4198 0.65 +0.3 13,440 + GFF4198 0.65 -0.7 13,521 + GFF4198 0.70 +0.2 13,521 + GFF4198 0.70 +0.3 13,576 + GFF4198 0.73 +0.3 13,682 - GFF4198 0.78 -0.2 14,014 - -0.3 14,097 + -0.8 14,097 + +0.2 14,098 - -0.3 14,098 - +0.3 14,321 + GFF4199 0.36 +0.0 14,321 + GFF4199 0.36 -0.2 14,322 - GFF4199 0.36 +0.1 14,430 - GFF4199 0.53 -0.1 14,466 + GFF4199 0.58 -0.1 14,467 - GFF4199 0.59 -0.2 14,467 - GFF4199 0.59 -0.7 14,467 - GFF4199 0.59 -0.6 14,549 + GFF4199 0.72 -0.1 14,660 + GFF4199 0.89 -0.2 14,813 + GFF4200 0.12 +0.2 14,813 + GFF4200 0.12 -0.1 14,814 - GFF4200 0.12 -0.2 15,194 + GFF4200 0.62 -0.8 15,194 + GFF4200 0.62 -0.3 15,195 - GFF4200 0.62 -0.5 15,662 + -0.1 15,662 + -0.3 15,667 + +0.6 15,667 + +0.3 15,667 + +0.3 15,668 - -0.0 15,671 + -0.1
Or see this region's nucleotide sequence