Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4175 and GFF4176 are separated by 439 nucleotides GFF4176 and GFF4177 are separated by 9 nucleotides
GFF4175 - Uncharacterized protein YfiR precursor, at 63,994 to 64,512
GFF4175
GFF4176 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 64,952 to 66,022
GFF4176
GFF4177 - Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43), at 66,032 to 67,153
GFF4177
Position (kb)
64
65
66
67 Strain fitness (log2 ratio)
-2
-1
0
1 at 64.102 kb on - strand, within GFF4175 at 64.156 kb on + strand, within GFF4175 at 64.346 kb on - strand, within GFF4175 at 64.352 kb on - strand, within GFF4175 at 64.386 kb on + strand, within GFF4175 at 64.386 kb on + strand, within GFF4175 at 64.387 kb on - strand, within GFF4175 at 64.388 kb on + strand, within GFF4175 at 64.552 kb on + strand at 64.820 kb on + strand at 64.820 kb on + strand at 64.820 kb on + strand at 64.821 kb on - strand at 64.978 kb on - strand at 64.999 kb on - strand at 65.020 kb on + strand at 65.062 kb on - strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.168 kb on + strand, within GFF4176 at 65.169 kb on - strand, within GFF4176 at 65.216 kb on + strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.728 kb on - strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.821 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.944 kb on + strand at 65.945 kb on - strand at 65.971 kb on - strand at 66.066 kb on + strand at 66.187 kb on + strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.278 kb on + strand, within GFF4177 at 66.539 kb on - strand, within GFF4177 at 66.938 kb on + strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.949 kb on + strand, within GFF4177 at 66.950 kb on - strand, within GFF4177 at 66.952 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 64,102 - GFF4175 0.21 -0.0 64,156 + GFF4175 0.31 -0.2 64,346 - GFF4175 0.68 -0.1 64,352 - GFF4175 0.69 +0.4 64,386 + GFF4175 0.76 +0.6 64,386 + GFF4175 0.76 +0.0 64,387 - GFF4175 0.76 +0.0 64,388 + GFF4175 0.76 -0.1 64,552 + -0.1 64,820 + -0.5 64,820 + -0.2 64,820 + -0.2 64,821 - +0.1 64,978 - +0.9 64,999 - +0.0 65,020 + -0.5 65,062 - GFF4176 0.10 +0.0 65,124 + GFF4176 0.16 -0.4 65,124 + GFF4176 0.16 -0.4 65,168 + GFF4176 0.20 -0.0 65,169 - GFF4176 0.20 -1.2 65,216 + GFF4176 0.25 -0.9 65,676 - GFF4176 0.68 -0.2 65,676 - GFF4176 0.68 +0.1 65,728 - GFF4176 0.72 -0.4 65,745 + GFF4176 0.74 -0.4 65,745 + GFF4176 0.74 +0.2 65,821 + GFF4176 0.81 -0.2 65,863 + GFF4176 0.85 +0.1 65,863 + GFF4176 0.85 -1.0 65,863 + GFF4176 0.85 +0.0 65,944 + +0.2 65,945 - +0.5 65,971 - +0.6 66,066 + +0.6 66,187 + GFF4177 0.14 -0.3 66,188 - GFF4177 0.14 +0.3 66,188 - GFF4177 0.14 -1.0 66,278 + GFF4177 0.22 +0.1 66,539 - GFF4177 0.45 +0.1 66,938 + GFF4177 0.81 +1.0 66,939 - GFF4177 0.81 -2.6 66,939 - GFF4177 0.81 +1.3 66,949 + GFF4177 0.82 +0.1 66,950 - GFF4177 0.82 -0.5 66,952 + GFF4177 0.82 -0.2 66,992 + GFF4177 0.86 +0.3 66,992 + GFF4177 0.86 +0.1 66,992 + GFF4177 0.86 +0.1
Or see this region's nucleotide sequence