Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4104 and GFF4105 are separated by 218 nucleotides GFF4105 and GFF4106 overlap by 4 nucleotides GFF4106 and GFF4107 overlap by 4 nucleotides GFF4107 and GFF4108 are separated by 155 nucleotides
GFF4104 - DNA polymerase IV (EC 2.7.7.7), at 63,210 to 64,265
GFF4104
GFF4105 - Protein with similarity to RtcB, at 64,484 to 65,458
GFF4105
GFF4106 - Protein with similarity to RtcB, at 65,455 to 65,616
GFF4106
GFF4107 - Peptide chain release factor homolog, at 65,613 to 66,227
GFF4107
GFF4108 - Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3), at 66,383 to 67,840
GFF4108
Position (kb) 64
65
66 Strain fitness (log2 ratio) -1
0
1
2 at 63.694 kb on - strand, within GFF4104 at 63.912 kb on - strand, within GFF4104 at 64.291 kb on - strand at 64.572 kb on + strand at 64.576 kb on + strand at 64.576 kb on + strand at 64.751 kb on - strand, within GFF4105 at 65.219 kb on - strand, within GFF4105 at 65.251 kb on + strand, within GFF4105 at 65.311 kb on + strand, within GFF4105 at 65.312 kb on - strand, within GFF4105 at 65.482 kb on + strand, within GFF4106 at 65.483 kb on - strand, within GFF4106 at 65.503 kb on + strand, within GFF4106 at 65.503 kb on + strand, within GFF4106 at 65.506 kb on - strand, within GFF4106 at 65.717 kb on - strand, within GFF4107 at 65.753 kb on - strand, within GFF4107 at 65.753 kb on - strand, within GFF4107 at 65.923 kb on + strand, within GFF4107 at 65.987 kb on + strand, within GFF4107 at 65.988 kb on - strand, within GFF4107 at 66.201 kb on - strand at 66.249 kb on - strand at 66.286 kb on + strand at 66.310 kb on + strand at 66.452 kb on + strand at 66.452 kb on + strand at 66.452 kb on + strand at 66.453 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 63,694 - GFF4104 0.46 +1.2 63,912 - GFF4104 0.66 +0.1 64,291 - +0.5 64,572 + +1.0 64,576 + -0.1 64,576 + -0.2 64,751 - GFF4105 0.27 -1.4 65,219 - GFF4105 0.75 -0.4 65,251 + GFF4105 0.79 -0.2 65,311 + GFF4105 0.85 -0.3 65,312 - GFF4105 0.85 -0.2 65,482 + GFF4106 0.17 +0.4 65,483 - GFF4106 0.17 -0.3 65,503 + GFF4106 0.30 +0.5 65,503 + GFF4106 0.30 +0.3 65,506 - GFF4106 0.31 +0.2 65,717 - GFF4107 0.17 -0.9 65,753 - GFF4107 0.23 +0.3 65,753 - GFF4107 0.23 +0.5 65,923 + GFF4107 0.50 +0.9 65,987 + GFF4107 0.61 -0.1 65,988 - GFF4107 0.61 -0.2 66,201 - -1.4 66,249 - +2.3 66,286 + +1.2 66,310 + -0.5 66,452 + +0.1 66,452 + -0.3 66,452 + +0.2 66,453 - -0.2
Or see this region's nucleotide sequence