Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3903 and GFF3904 are separated by 236 nucleotides GFF3904 and GFF3905 are separated by 142 nucleotides
GFF3903 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 184,086 to 185,492
GFF3903
GFF3904 - UDP-glucose 6-dehydrogenase (EC 1.1.1.22), at 185,729 to 186,895
GFF3904
GFF3905 - regulator of length of O-antigen component of lipopolysaccharide chains, at 187,038 to 188,021
GFF3905
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1 at 184.773 kb on + strand, within GFF3903 at 184.790 kb on + strand, within GFF3903 at 184.947 kb on + strand, within GFF3903 at 185.082 kb on + strand, within GFF3903 at 185.082 kb on + strand, within GFF3903 at 185.240 kb on + strand, within GFF3903 at 185.367 kb on + strand at 185.900 kb on - strand, within GFF3904 at 186.247 kb on - strand, within GFF3904 at 186.336 kb on - strand, within GFF3904 at 186.377 kb on + strand, within GFF3904 at 186.377 kb on + strand, within GFF3904 at 186.377 kb on + strand, within GFF3904 at 186.454 kb on + strand, within GFF3904 at 186.542 kb on - strand, within GFF3904 at 186.732 kb on - strand, within GFF3904 at 186.732 kb on - strand, within GFF3904 at 186.754 kb on + strand, within GFF3904 at 186.763 kb on + strand, within GFF3904 at 186.763 kb on + strand, within GFF3904 at 186.990 kb on + strand at 186.996 kb on + strand at 187.028 kb on + strand at 187.037 kb on + strand at 187.052 kb on + strand at 187.220 kb on + strand, within GFF3905 at 187.254 kb on + strand, within GFF3905 at 187.257 kb on - strand, within GFF3905 at 187.335 kb on - strand, within GFF3905 at 187.335 kb on - strand, within GFF3905 at 187.443 kb on + strand, within GFF3905 at 187.488 kb on + strand, within GFF3905 at 187.544 kb on + strand, within GFF3905 at 187.628 kb on + strand, within GFF3905 at 187.629 kb on - strand, within GFF3905 at 187.872 kb on + strand, within GFF3905
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 184,773 + GFF3903 0.49 +0.4 184,790 + GFF3903 0.50 -0.1 184,947 + GFF3903 0.61 +0.6 185,082 + GFF3903 0.71 +0.3 185,082 + GFF3903 0.71 -1.8 185,240 + GFF3903 0.82 -0.4 185,367 + -0.0 185,900 - GFF3904 0.15 -2.1 186,247 - GFF3904 0.44 -0.1 186,336 - GFF3904 0.52 +0.7 186,377 + GFF3904 0.56 +0.0 186,377 + GFF3904 0.56 -0.9 186,377 + GFF3904 0.56 -0.3 186,454 + GFF3904 0.62 -0.0 186,542 - GFF3904 0.70 -0.6 186,732 - GFF3904 0.86 -0.4 186,732 - GFF3904 0.86 -0.0 186,754 + GFF3904 0.88 +0.1 186,763 + GFF3904 0.89 +0.0 186,763 + GFF3904 0.89 -0.8 186,990 + -0.0 186,996 + +0.4 187,028 + +0.0 187,037 + +0.7 187,052 + +0.0 187,220 + GFF3905 0.18 +0.9 187,254 + GFF3905 0.22 -0.1 187,257 - GFF3905 0.22 +1.7 187,335 - GFF3905 0.30 -0.7 187,335 - GFF3905 0.30 +0.3 187,443 + GFF3905 0.41 +0.2 187,488 + GFF3905 0.46 -0.0 187,544 + GFF3905 0.51 +1.1 187,628 + GFF3905 0.60 -0.0 187,629 - GFF3905 0.60 -1.3 187,872 + GFF3905 0.85 +0.2
Or see this region's nucleotide sequence