Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF361 and GFF362 overlap by 4 nucleotides GFF362 and GFF363 are separated by 250 nucleotides GFF363 and GFF364 are separated by 88 nucleotides
GFF361 - DNA ligase (EC 6.5.1.2), LigB, at 80,237 to 81,922
GFF361
GFF362 - Putative inner membrane protein, at 81,919 to 82,536
GFF362
GFF363 - Putative beta-lactamase, at 82,787 to 83,668
GFF363
GFF364 - Transcriptional regulator, LysR family, at 83,757 to 84,644
GFF364
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-2
-1
0
1 at 81.807 kb on + strand at 81.856 kb on + strand at 81.902 kb on - strand at 81.920 kb on + strand at 81.920 kb on + strand at 81.921 kb on - strand at 81.921 kb on - strand at 81.980 kb on - strand at 81.980 kb on - strand at 81.990 kb on + strand, within GFF362 at 82.003 kb on + strand, within GFF362 at 82.004 kb on - strand, within GFF362 at 82.009 kb on - strand, within GFF362 at 82.014 kb on + strand, within GFF362 at 82.041 kb on - strand, within GFF362 at 82.058 kb on + strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.093 kb on + strand, within GFF362 at 82.093 kb on + strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.134 kb on + strand, within GFF362 at 82.165 kb on - strand, within GFF362 at 82.217 kb on - strand, within GFF362 at 82.217 kb on - strand, within GFF362 at 82.217 kb on - strand, within GFF362 at 82.283 kb on - strand, within GFF362 at 82.338 kb on + strand, within GFF362 at 82.339 kb on - strand, within GFF362 at 82.346 kb on + strand, within GFF362 at 82.434 kb on + strand, within GFF362 at 82.438 kb on - strand, within GFF362 at 82.438 kb on - strand, within GFF362 at 82.438 kb on - strand, within GFF362 at 82.513 kb on + strand at 82.513 kb on + strand at 82.780 kb on + strand at 82.781 kb on - strand at 82.842 kb on + strand at 82.843 kb on - strand at 82.843 kb on - strand at 82.843 kb on - strand at 82.879 kb on + strand, within GFF363 at 82.991 kb on - strand, within GFF363 at 83.023 kb on + strand, within GFF363 at 83.023 kb on + strand, within GFF363 at 83.024 kb on - strand, within GFF363 at 83.053 kb on + strand, within GFF363 at 83.061 kb on + strand, within GFF363 at 83.132 kb on + strand, within GFF363 at 83.132 kb on + strand, within GFF363 at 83.362 kb on - strand, within GFF363 at 83.443 kb on + strand, within GFF363 at 83.477 kb on - strand, within GFF363 at 83.477 kb on - strand, within GFF363 at 83.502 kb on + strand, within GFF363 at 83.503 kb on - strand, within GFF363 at 83.534 kb on + strand, within GFF363 at 83.536 kb on + strand, within GFF363 at 83.536 kb on + strand, within GFF363 at 83.536 kb on + strand, within GFF363 at 83.542 kb on + strand, within GFF363 at 83.542 kb on + strand, within GFF363 at 83.554 kb on + strand, within GFF363 at 83.601 kb on - strand at 83.642 kb on + strand at 83.642 kb on + strand at 83.642 kb on + strand at 83.642 kb on + strand at 83.643 kb on - strand at 83.643 kb on - strand at 83.669 kb on - strand at 83.699 kb on + strand at 83.699 kb on + strand at 83.714 kb on + strand at 83.779 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.785 kb on - strand at 83.785 kb on - strand at 84.034 kb on + strand, within GFF364 at 84.034 kb on + strand, within GFF364 at 84.063 kb on - strand, within GFF364 at 84.216 kb on + strand, within GFF364 at 84.217 kb on - strand, within GFF364 at 84.311 kb on - strand, within GFF364 at 84.318 kb on + strand, within GFF364 at 84.331 kb on - strand, within GFF364 at 84.423 kb on + strand, within GFF364 at 84.472 kb on + strand, within GFF364 at 84.611 kb on + strand at 84.633 kb on + strand at 84.637 kb on - strand at 84.660 kb on + strand at 84.661 kb on - strand at 84.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 81,807 + +0.1 81,856 + -0.4 81,902 - +0.1 81,920 + +0.6 81,920 + +0.0 81,921 - +0.0 81,921 - +0.0 81,980 - +0.0 81,980 - -0.3 81,990 + GFF362 0.11 -0.2 82,003 + GFF362 0.14 +0.4 82,004 - GFF362 0.14 -0.4 82,009 - GFF362 0.15 -1.4 82,014 + GFF362 0.15 +0.2 82,041 - GFF362 0.20 +0.1 82,058 + GFF362 0.22 +1.3 82,059 - GFF362 0.23 -0.1 82,059 - GFF362 0.23 -0.5 82,059 - GFF362 0.23 -0.1 82,059 - GFF362 0.23 -0.2 82,059 - GFF362 0.23 -0.2 82,093 + GFF362 0.28 -0.2 82,093 + GFF362 0.28 +0.6 82,094 - GFF362 0.28 -0.0 82,094 - GFF362 0.28 +0.2 82,094 - GFF362 0.28 +0.3 82,094 - GFF362 0.28 -0.1 82,094 - GFF362 0.28 -0.7 82,134 + GFF362 0.35 +0.6 82,165 - GFF362 0.40 -0.3 82,217 - GFF362 0.48 -0.2 82,217 - GFF362 0.48 -0.1 82,217 - GFF362 0.48 +0.3 82,283 - GFF362 0.59 -0.1 82,338 + GFF362 0.68 -0.4 82,339 - GFF362 0.68 +0.4 82,346 + GFF362 0.69 -0.9 82,434 + GFF362 0.83 -0.3 82,438 - GFF362 0.84 +0.5 82,438 - GFF362 0.84 -0.3 82,438 - GFF362 0.84 -2.2 82,513 + -0.1 82,513 + +0.1 82,780 + +0.1 82,781 - -0.4 82,842 + +0.4 82,843 - +1.2 82,843 - -0.7 82,843 - +0.0 82,879 + GFF363 0.10 -0.3 82,991 - GFF363 0.23 -1.6 83,023 + GFF363 0.27 +0.2 83,023 + GFF363 0.27 +0.7 83,024 - GFF363 0.27 -0.1 83,053 + GFF363 0.30 -0.7 83,061 + GFF363 0.31 -0.4 83,132 + GFF363 0.39 -0.3 83,132 + GFF363 0.39 +1.4 83,362 - GFF363 0.65 -0.5 83,443 + GFF363 0.74 -0.5 83,477 - GFF363 0.78 -0.1 83,477 - GFF363 0.78 -0.2 83,502 + GFF363 0.81 -0.3 83,503 - GFF363 0.81 -0.4 83,534 + GFF363 0.85 -0.0 83,536 + GFF363 0.85 +0.2 83,536 + GFF363 0.85 -0.9 83,536 + GFF363 0.85 -0.4 83,542 + GFF363 0.86 +0.8 83,542 + GFF363 0.86 +0.5 83,554 + GFF363 0.87 +0.5 83,601 - -0.3 83,642 + -0.3 83,642 + +0.1 83,642 + -2.6 83,642 + -0.1 83,643 - +0.1 83,643 - +0.2 83,669 - -0.2 83,699 + +0.1 83,699 + -0.3 83,714 + +0.3 83,779 + -0.0 83,784 + -0.9 83,784 + -0.4 83,784 + +0.5 83,784 + +1.0 83,784 + +0.4 83,785 - +0.0 83,785 - +0.6 84,034 + GFF364 0.31 -0.5 84,034 + GFF364 0.31 -0.0 84,063 - GFF364 0.34 -0.5 84,216 + GFF364 0.52 +0.0 84,217 - GFF364 0.52 -0.6 84,311 - GFF364 0.62 -0.6 84,318 + GFF364 0.63 -0.4 84,331 - GFF364 0.65 +0.1 84,423 + GFF364 0.75 +0.2 84,472 + GFF364 0.81 -0.0 84,611 + -0.4 84,633 + +0.4 84,637 - +0.5 84,660 + -0.3 84,661 - +0.1 84,661 - -0.2
Or see this region's nucleotide sequence