Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2648 and GFF2649 are separated by 43 nucleotides GFF2649 and GFF2650 are separated by 486 nucleotides
GFF2648 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 172,927 to 173,862
GFF2648
GFF2649 - ATP-dependent 23S rRNA helicase DbpA, at 173,906 to 175,279
GFF2649
GFF2650 - Zinc transport protein ZntB, at 175,766 to 176,749
GFF2650
Position (kb) 173
174
175
176 Strain fitness (log2 ratio) -1
0
1 at 173.079 kb on + strand, within GFF2648 at 173.080 kb on - strand, within GFF2648 at 173.080 kb on - strand, within GFF2648 at 173.080 kb on - strand, within GFF2648 at 173.083 kb on + strand, within GFF2648 at 173.084 kb on - strand, within GFF2648 at 173.085 kb on + strand, within GFF2648 at 173.187 kb on + strand, within GFF2648 at 173.188 kb on - strand, within GFF2648 at 173.200 kb on + strand, within GFF2648 at 173.512 kb on - strand, within GFF2648 at 173.656 kb on - strand, within GFF2648 at 174.038 kb on - strand at 174.334 kb on - strand, within GFF2649 at 174.358 kb on + strand, within GFF2649 at 174.497 kb on - strand, within GFF2649 at 174.572 kb on - strand, within GFF2649 at 174.651 kb on - strand, within GFF2649 at 174.671 kb on + strand, within GFF2649 at 174.671 kb on + strand, within GFF2649 at 174.671 kb on + strand, within GFF2649 at 174.686 kb on + strand, within GFF2649 at 175.056 kb on - strand, within GFF2649 at 175.056 kb on - strand, within GFF2649 at 175.462 kb on - strand at 175.467 kb on + strand at 175.467 kb on + strand at 175.468 kb on - strand at 175.468 kb on - strand at 175.491 kb on - strand at 175.491 kb on - strand at 175.543 kb on - strand at 175.543 kb on - strand at 175.563 kb on + strand at 175.582 kb on - strand at 175.945 kb on + strand, within GFF2650 at 175.947 kb on + strand, within GFF2650 at 176.127 kb on + strand, within GFF2650 at 176.151 kb on + strand, within GFF2650 at 176.180 kb on + strand, within GFF2650 at 176.267 kb on - strand, within GFF2650
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 173,079 + GFF2648 0.16 +0.6 173,080 - GFF2648 0.16 +0.1 173,080 - GFF2648 0.16 -0.2 173,080 - GFF2648 0.16 +0.1 173,083 + GFF2648 0.17 +0.6 173,084 - GFF2648 0.17 +0.1 173,085 + GFF2648 0.17 -0.3 173,187 + GFF2648 0.28 +0.2 173,188 - GFF2648 0.28 -0.6 173,200 + GFF2648 0.29 +0.3 173,512 - GFF2648 0.62 +1.0 173,656 - GFF2648 0.78 -0.5 174,038 - -0.0 174,334 - GFF2649 0.31 -0.1 174,358 + GFF2649 0.33 -0.5 174,497 - GFF2649 0.43 +0.4 174,572 - GFF2649 0.48 +0.0 174,651 - GFF2649 0.54 -0.6 174,671 + GFF2649 0.56 +0.3 174,671 + GFF2649 0.56 +0.4 174,671 + GFF2649 0.56 -0.6 174,686 + GFF2649 0.57 -0.2 175,056 - GFF2649 0.84 +0.4 175,056 - GFF2649 0.84 -0.5 175,462 - +0.0 175,467 + -0.0 175,467 + -0.1 175,468 - +0.6 175,468 - +0.0 175,491 - -0.2 175,491 - -0.4 175,543 - -0.3 175,543 - -0.4 175,563 + -0.7 175,582 - -0.3 175,945 + GFF2650 0.18 -0.2 175,947 + GFF2650 0.18 +0.7 176,127 + GFF2650 0.37 -0.2 176,151 + GFF2650 0.39 +0.1 176,180 + GFF2650 0.42 +0.2 176,267 - GFF2650 0.51 -0.1
Or see this region's nucleotide sequence