Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2497 and GFF2498 are separated by 138 nucleotides GFF2498 and GFF2499 are separated by 344 nucleotides GFF2499 and GFF2500 are separated by 119 nucleotides
GFF2497 - 3-hydroxypropionate dehydrogenase (EC 1.1.1.298), at 14,419 to 15,165
GFF2497
GFF2498 - Dipeptidyl carboxypeptidase Dcp (EC 3.4.15.5), at 15,304 to 17,346
GFF2498
GFF2499 - Conidiation-specific protein 10, at 17,691 to 17,873
GFF2499
GFF2500 - C-terminal domain of CinA paralog, YdeJ, at 17,993 to 18,520
GFF2500
Position (kb) 15
16
17
18 Strain fitness (log2 ratio) -1
0
1 at 14.347 kb on + strand at 14.374 kb on + strand at 14.413 kb on - strand at 14.420 kb on + strand at 14.557 kb on - strand, within GFF2497 at 14.727 kb on - strand, within GFF2497 at 15.090 kb on + strand, within GFF2497 at 15.152 kb on - strand at 15.195 kb on + strand at 15.195 kb on + strand at 15.199 kb on - strand at 15.203 kb on + strand at 15.228 kb on - strand at 15.333 kb on + strand at 15.333 kb on + strand at 15.616 kb on - strand, within GFF2498 at 15.887 kb on + strand, within GFF2498 at 16.141 kb on + strand, within GFF2498 at 16.430 kb on + strand, within GFF2498 at 16.431 kb on - strand, within GFF2498 at 16.431 kb on - strand, within GFF2498 at 16.530 kb on - strand, within GFF2498 at 16.628 kb on + strand, within GFF2498 at 16.631 kb on - strand, within GFF2498 at 16.642 kb on - strand, within GFF2498 at 16.941 kb on + strand, within GFF2498 at 17.003 kb on + strand, within GFF2498 at 17.138 kb on - strand, within GFF2498 at 17.143 kb on + strand at 18.287 kb on + strand, within GFF2500 at 18.331 kb on - strand, within GFF2500 at 18.331 kb on - strand, within GFF2500
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 14,347 + -0.5 14,374 + +0.1 14,413 - -0.4 14,420 + -0.1 14,557 - GFF2497 0.18 -1.1 14,727 - GFF2497 0.41 +0.5 15,090 + GFF2497 0.90 -0.7 15,152 - +0.5 15,195 + -0.8 15,195 + -0.1 15,199 - -0.2 15,203 + +0.0 15,228 - +0.7 15,333 + +0.3 15,333 + +0.0 15,616 - GFF2498 0.15 -0.6 15,887 + GFF2498 0.29 +0.5 16,141 + GFF2498 0.41 +0.0 16,430 + GFF2498 0.55 +0.8 16,431 - GFF2498 0.55 -0.3 16,431 - GFF2498 0.55 -0.3 16,530 - GFF2498 0.60 -0.5 16,628 + GFF2498 0.65 -1.3 16,631 - GFF2498 0.65 -0.3 16,642 - GFF2498 0.65 -0.4 16,941 + GFF2498 0.80 -0.9 17,003 + GFF2498 0.83 -0.1 17,138 - GFF2498 0.90 +0.4 17,143 + +0.0 18,287 + GFF2500 0.56 +0.0 18,331 - GFF2500 0.64 -0.6 18,331 - GFF2500 0.64 +0.3
Or see this region's nucleotide sequence