Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2465 and GFF2466 are separated by 115 nucleotides GFF2466 and GFF2467 are separated by 38 nucleotides GFF2467 and GFF2468 are separated by 21 nucleotides
GFF2465 - N-acetylmuramic acid 6-phosphate etherase, at 85,064 to 85,957
GFF2465
GFF2466 - Sialic acid utilization regulator, RpiR family, at 86,073 to 86,921
GFF2466
GFF2467 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 86,960 to 87,877
GFF2467
GFF2468 - Putative transmembrane transport protein, at 87,899 to 89,095
GFF2468
Position (kb) 86
87 Strain fitness (log2 ratio) -1
0
1 at 85.299 kb on + strand, within GFF2465 at 85.339 kb on + strand, within GFF2465 at 85.427 kb on - strand, within GFF2465 at 85.429 kb on + strand, within GFF2465 at 85.521 kb on + strand, within GFF2465 at 85.757 kb on - strand, within GFF2465 at 85.766 kb on - strand, within GFF2465 at 85.855 kb on + strand, within GFF2465 at 85.868 kb on - strand at 85.876 kb on + strand at 85.891 kb on + strand at 85.941 kb on - strand at 86.090 kb on + strand at 86.377 kb on + strand, within GFF2466 at 86.851 kb on + strand at 86.883 kb on - strand at 86.962 kb on - strand at 87.019 kb on + strand at 87.020 kb on - strand at 87.056 kb on + strand, within GFF2467 at 87.057 kb on - strand, within GFF2467 at 87.089 kb on + strand, within GFF2467 at 87.089 kb on + strand, within GFF2467 at 87.104 kb on - strand, within GFF2467 at 87.104 kb on - strand, within GFF2467 at 87.104 kb on - strand, within GFF2467 at 87.274 kb on + strand, within GFF2467 at 87.295 kb on - strand, within GFF2467 at 87.345 kb on - strand, within GFF2467 at 87.379 kb on + strand, within GFF2467 at 87.572 kb on - strand, within GFF2467 at 87.617 kb on - strand, within GFF2467 at 87.632 kb on - strand, within GFF2467 at 87.718 kb on - strand, within GFF2467 at 87.718 kb on - strand, within GFF2467 at 87.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 85,299 + GFF2465 0.26 -0.9 85,339 + GFF2465 0.31 +0.1 85,427 - GFF2465 0.41 -0.2 85,429 + GFF2465 0.41 +0.5 85,521 + GFF2465 0.51 +0.1 85,757 - GFF2465 0.78 -0.1 85,766 - GFF2465 0.79 +0.2 85,855 + GFF2465 0.88 +0.7 85,868 - -0.2 85,876 + -0.4 85,891 + -0.1 85,941 - +0.6 86,090 + +0.3 86,377 + GFF2466 0.36 +0.1 86,851 + +0.1 86,883 - +0.9 86,962 - +0.6 87,019 + +0.2 87,020 - -0.0 87,056 + GFF2467 0.10 +0.6 87,057 - GFF2467 0.11 -0.0 87,089 + GFF2467 0.14 +0.7 87,089 + GFF2467 0.14 -0.5 87,104 - GFF2467 0.16 +1.1 87,104 - GFF2467 0.16 +0.0 87,104 - GFF2467 0.16 +0.1 87,274 + GFF2467 0.34 +0.5 87,295 - GFF2467 0.36 -0.6 87,345 - GFF2467 0.42 +0.9 87,379 + GFF2467 0.46 +0.0 87,572 - GFF2467 0.67 +0.9 87,617 - GFF2467 0.72 +0.7 87,632 - GFF2467 0.73 +0.2 87,718 - GFF2467 0.83 +0.2 87,718 - GFF2467 0.83 -0.5 87,794 - +0.2
Or see this region's nucleotide sequence