Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2273 and GFF2274 overlap by 8 nucleotides GFF2274 and GFF2275 are separated by 18 nucleotides GFF2275 and GFF2276 are separated by 328 nucleotides
GFF2273 - Predicted rhamnogalacturonide-specific TRAP-type transporter, large transmembrane component RhiC, at 62,317 to 63,624
GFF2273
GFF2274 - Predicted rhamnogalacturonide-specific TRAP-type transporter, small transmembrane component RhiB, at 63,617 to 64,132
GFF2274
GFF2275 - Predicted rhamnogalacturonide-specific TRAP-type transporter, substrate-binding component RhiA, at 64,151 to 65,134
GFF2275
GFF2276 - Manganese superoxide dismutase (EC 1.15.1.1), at 65,463 to 66,083
GFF2276
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 63.301 kb on - strand, within GFF2273 at 63.319 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.592 kb on + strand at 63.592 kb on + strand at 63.598 kb on - strand at 63.613 kb on - strand at 63.705 kb on + strand, within GFF2274 at 63.705 kb on + strand, within GFF2274 at 63.925 kb on + strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 64.104 kb on - strand at 64.148 kb on - strand at 64.286 kb on + strand, within GFF2275 at 64.412 kb on - strand, within GFF2275 at 64.458 kb on + strand, within GFF2275 at 64.459 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.549 kb on - strand, within GFF2275 at 64.646 kb on + strand, within GFF2275 at 64.682 kb on - strand, within GFF2275 at 64.715 kb on + strand, within GFF2275 at 64.749 kb on + strand, within GFF2275 at 64.877 kb on - strand, within GFF2275 at 64.951 kb on - strand, within GFF2275 at 64.951 kb on - strand, within GFF2275 at 64.955 kb on + strand, within GFF2275 at 64.956 kb on - strand, within GFF2275 at 65.094 kb on - strand at 65.115 kb on - strand at 65.115 kb on - strand at 65.117 kb on - strand at 65.402 kb on + strand at 65.462 kb on + strand at 65.469 kb on + strand at 65.471 kb on + strand at 65.472 kb on - strand at 65.565 kb on + strand, within GFF2276 at 65.566 kb on - strand, within GFF2276 at 65.985 kb on + strand, within GFF2276 at 65.986 kb on - strand, within GFF2276
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 63,301 - GFF2273 0.75 +1.9 63,319 - GFF2273 0.77 -0.1 63,321 - GFF2273 0.77 +0.8 63,321 - GFF2273 0.77 -0.1 63,321 - GFF2273 0.77 +0.1 63,592 + -0.3 63,592 + +0.1 63,598 - +0.6 63,613 - -2.6 63,705 + GFF2274 0.17 -0.0 63,705 + GFF2274 0.17 +0.1 63,925 + GFF2274 0.60 +0.5 63,926 - GFF2274 0.60 +0.9 63,926 - GFF2274 0.60 +0.3 63,926 - GFF2274 0.60 +0.2 64,104 - +0.2 64,148 - +0.2 64,286 + GFF2275 0.14 +0.2 64,412 - GFF2275 0.27 +0.5 64,458 + GFF2275 0.31 -0.1 64,459 - GFF2275 0.31 -0.0 64,539 - GFF2275 0.39 +0.2 64,539 - GFF2275 0.39 +0.2 64,546 + GFF2275 0.40 -0.3 64,546 + GFF2275 0.40 +0.3 64,547 - GFF2275 0.40 +0.2 64,547 - GFF2275 0.40 -0.4 64,548 + GFF2275 0.40 -0.2 64,548 + GFF2275 0.40 +0.1 64,549 - GFF2275 0.40 +0.8 64,646 + GFF2275 0.50 +0.5 64,682 - GFF2275 0.54 +0.0 64,715 + GFF2275 0.57 +0.0 64,749 + GFF2275 0.61 +0.2 64,877 - GFF2275 0.74 -0.3 64,951 - GFF2275 0.81 -0.1 64,951 - GFF2275 0.81 +0.1 64,955 + GFF2275 0.82 -0.6 64,956 - GFF2275 0.82 +0.3 65,094 - -0.8 65,115 - -0.5 65,115 - -0.2 65,117 - +0.4 65,402 + +0.4 65,462 + +0.0 65,469 + -0.3 65,471 + +1.1 65,472 - -0.8 65,565 + GFF2276 0.16 +0.3 65,566 - GFF2276 0.17 -0.5 65,985 + GFF2276 0.84 +0.3 65,986 - GFF2276 0.84 -0.0
Or see this region's nucleotide sequence